APC-H-2Kb/MuLV p15E (KSPWFTTL) MHC Tetramer (CAT#: MHC-LC521)

1. Q 1: What effects does the tetramer's APC conjugation have on its fluorescence in flow cytometry?

   A: Using flow cytometry, the APC conjugation produces a unique fluorescent signal that helps with the accurate identification of T cells that are specific to an antigen. Its robust photostability and high quantum yield guarantee consistent and dependable T cell profiling outcomes.

2. Q 2: What is the compatibility of the APC-H-2Kb/MuLV p15E Tetramer with intracellular cytokine staining?

   A: Indeed, following surface tetramer labeling, cells may be fixed and permeabilized to facilitate intracellular cytokine analysis. It is essential to thoroughly monitor for any potential attenuation of fluorescent signals throughout the intracellular staining process.

3. Q 3: How many cells per range should I stain with this tetramer in order to prevent non-specific binding?

   A: For best staining, a cell density of 1x10^6 to 5x10^6 cells per 100 µL of staining buffer is advised. Reduced resolution of the antigen-specific T cell populations and non-specific binding are possible outcomes of cell overload.

View the frequently asked questions answered by Creative Biolabs Support.