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    APC-HLA-G/Human H2AFX (RIIPRHLQL) MHC Tetramer (CAT#: MHC-LC1326)

    This product is a tetramer of biotinylated peptide/MHC complex with streptavidin mainly composed of H2AFX-derived peptide of RIIPRHLQL covering 78-86 and HLA-G molecule. The pMHC tetramer recognizes CD8 T cells, and can be used in the analysis of individual antigen-specific T cells.

    Specific Inquiry
    • Size:
    Custom Production

    MHC tetramer custom production provides a vital tool for researchers seeking to understand and manipulate T-cell immunity with exceptional precision. Please specify your requirements, including peptide sequence, MHC allele, and desired fluorophore etc. We will respond as soon as possible with a tailored solution.

    Peptide Sequence *:
    Allele Requested *:
    Antigen Species:
    Antigen Molecule:
    Sequence Position:
    Form Requested *:
    Conjugation *:
    Size *:
    Comments:

    Disclaimer: Please note that the MHC reagents we offered have been produced with strict quality control (including gene sequencing, affinity purification, SDS-PAGE analysis, and verification of biotinylation), but many have not been confirmed to stain or activate specific T cells.

    We require custom production
    • Gene Expression
    • Datasheet
    • MSDS
    • COA
    Subcellular Location
    Figure 1 IF staining of human cell line A-431
    Figure 1 IF staining of human cell line A-431
    (Immunofluorescent staining of human cell line A-431 shows localization to nucleoplasm & nuclear speckles.)
    * Image credit: Human Protein Atlas v21.proteinatlas.org/images/51647/769_B5_3_selected.jpg
    Figure 2 IF staining of human cell line U-2 OS
    Figure 2 IF staining of human cell line U-2 OS
    (Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm.)
    * Image credit: Human Protein Atlas v21.proteinatlas.org/images/41189/1912_D10_11_cr5c862af3f3e0f_selected.jpg
    Normal Tissue
    Figure 3 IHC staining of human tonsil
    Figure 3 IHC staining of human tonsil
    (Immunohistochemical staining of human tonsil shows nuclear positivity in germinal center cells,non-germinal center cells and squamous epithelial cells.)
    * Image credit: Human Protein Atlas v21.proteinatlas.org/images/51647/166885_A_5_8_selected.jpg
    Figure 4 Lymph node
    Figure 4 Lymph node
    (Germinal center cells Staining: High Intensity: Strong Quantity:>75% Location: nuclear Non-germinal center cells Staining: High Intensity: Strong Quantity:>75% Location: Nuclear)
    * Image credit: Human Protein Atlas v21.proteinatlas.org/images/12264/32029_A_9_8.jpg
    Figure 5 Cerebral cortex
    Figure 5 Cerebral cortex
    (Endothelial cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Nuclear Glial cells Staining: High Intensity: Strong Quantity:>75% Location: nuclear Neuronal cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous nuclear)
    * Image credit: Human Protein Atlas v21.proteinatlas.org/images/12264/32029_B_9_5.jpg
    Figure 6 Colon
    Figure 6 Colon
    (Endothelial cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Nuclear Glandular cells Staining: High Intensity: Strong Quantity:>75% Location: Nuclear Peripheral nerve/ganglion Staining: Medium Intensity: Moderate Quantity:>75% Location: Nuclear)
    * Image credit: Human Protein Atlas v21.proteinatlas.org/images/12264/32029_A_7_3.jpg
    Figure 7 Liver
    Figure 7 Liver
    (Cholangiocytes Staining: High Intensity: Strong Quantity:>75% Location: Nuclear Hepatocytes Staining: High Intensity: Strong Quantity:>75% Location: Nuclear)
    * Image credit: Human Protein Atlas v21.proteinatlas.org/images/12264/32029_A_7_4.jpg
    Figure 8 Kidney
    Figure 8 Kidney
    (Bowman's capsule Staining: High Intensity: Strong Quantity:>75% Cells in glomeruli Staining: High Intensity: Strong Quantity: 75%-25% Collecting ducts Staining: High Intensity: Strong Quantity:>75% Distal tubules Staining: High Intensity: Strong Quantity:>75%)
    * Image credit: Human Protein Atlas v21.proteinatlas.org/images/12264/32029_A_7_5.jpg
    Figure 9 Testis
    Figure 9 Testis
    (Cells in seminiferous ducts Staining: High Intensity: Strong Quantity:>75% Location: nuclear Leydig cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Nuclear)
    * Image credit: Human Protein Atlas v21.proteinatlas.org/images/12264/32029_A_6_6.jpg
    Figure 10 Skin
    Figure 10 Skin
    (Cells in basal layer Staining: Medium Intensity: Moderate Quantity:>75% Cells in granular layer Staining: Medium Intensity: Moderate Quantity:>75% Cells in spinous layer Staining: Medium Intensity: Moderate Quantity:>75% Endothelial cells Staining: Medium Intensity: Moderate Fibrohistiocytic cells Staining: High Intensity: Strong Langerhans cells Staining: Medium Intensity: Moderate Quantity:>75% Lymphocytes Staining: High Intensity: Strong Melanocytes Staining: High Intensity: Strong Quantity: 75%-25%)
    * Image credit: Human Protein Atlas v21.proteinatlas.org/images/12264/32029_B_9_1.jpg
    RNA Expression
    Figure 11 RNA cell line category: Low cell line specificity
    Figure 11 RNA cell line category: Low cell line specificity
    (Cell lines ordered by descending RNA expression order)
    * Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000188486-H2AFX

    Specifications

    • Allele
    • HLA-G
    • Class
    • Class I
    • MHC Species
    • Human
    • Antigen
    • H2AFX
    • Antigen Species
    • Human
    • Peptide
    • RIIPRHLQL
    • Range
    • 78-86
    • Conjugate
    • APC
    • Application
    • FCM

    Target

    • Antigen Introduction
    • Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. The linker histone, H1, interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into higher order structures. This gene encodes a replication-independent histone that is a member of the histone H2A family, and generates two transcripts through the use of the conserved stem-loop termination motif, and the polyA addition motif.
    • Alternative Names
    • H2AFX; H2A histone family member X; H2AX; H2A.X; H2A/X

    Downloads

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    CAT Product Name Application Type
    MHC-LC1325 PE-HLA-G/Human H2AFX (RIIPRHLQL) MHC Tetramer FCM
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