CBGlyco™ Activated glutaraldehyde HRP Labeling Kit (CAT#: Glyco-034CL)

Activated Peroxidase and Antibody Labeling Kit is designed to prepare and purify HRP-conjugated antibodies with any previously unmodified primary or secondary antibody (IgG).

  • Datasheet
  • MSDS
  • COA

Specifications

  • Product Size
  • 5 reactions
  • Label or Dye
  • HRP (Horseradish Peroxidase)
  • Labeling Scale
  • 300 µg
  • Labeling Target
  • Antibodies (IgG)
  • Contents & storage
  • Sufficient For: 5 conjugations, each with 0.3 mg antibody (IgG)
    • Activated Peroxidase, 5 mg
    • Conjugation Buffer, 50 mL
    • Lysine, 250 mg
    • Protein A/G Column, 0.5 mL
    • Gentle Ag/Ab Binding Buffer, 200 mL
    • Gentle Ag/Ab Elution Buffer, 200 mL

    Store activated peroxidase at -20°C. Store other components at 4°C.

Background

  • Description
  • Activated Peroxidase is HRP enzyme that has been activated with glutaraldehyde to contain reactive groups that will spontaneously conjugate with amines on an antibody (or other protein). Incubation of this Activated Peroxidase with IgG in sodium carbonate buffer (pH 9.5) results in formation of a conjugate where both the enzyme activity of HRP and the antigen-binding activity of IgG are preserved. The Antibody Labeling Kit includes the Activated Peroxidase and a Protein A/G column and buffers to purify the antibody conjugate from excess, unreacted HRP enzyme.

For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.

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* Abbreviations
3D IHC3D Immunohistochemistry
ActivActivation
AgonistAgonist
ApopApoptosis
BABioassay
BIBioimaging
BlockBlocking
Cell ScreeningCell Screening
SeparationCell Separation
ChIPChromatin Immunoprecipitation
CMCDComplement Mediated Cell Depletion
CostimCostimulation
CytCytotoxicity
DepletionDepletion
DBDot Blot
EMElectron Microscopy
ELISAEnzyme-linked Immunosorbent Assay
ELISPOTEnzyme-linked Immunosorbent Spot
FCFlow Cytometry
FuncSFunctional Assay
GSGel Super Shift Assay
HAHemagglutination
IAImmunoassay
IBImmunoblotting
ICCImmunocytochemistry
IDImmunodiffusion
IFImmunofluorescence
IHCImmunohistochemistry
IHC-FrImmunohistochemistry-Frozen
IHC-PImmunohistochemistry-Paraffin
REImmunohistology - Resin Sections
IPImmunoprecipitation
IRMAImmunoradiometric Assay
SHIn situ hybridization
InhibInhibition
ICFCIntracellular Staining for Flow Cytometry
KO/KD-WBKnockout/Knockdown target confirmation by Western Blot
Live cell imagingLive cell imaging
CyTOF®Mass Cytometry
MeDIPMethylated DNA Immunoprecipitation
MultiplexMultiplex bead-based assay
NeutNeutralization
PPProtein Purification
PGProteogenomics
RIRadial Immunodiffusion
RIARadioimmunoassay
StimStimulation
SPRSurface Plasmon Resonance
TCTissue Culture
TBTurbidimetry
WBWestern Blot

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