CBGlyco™ Aminolink Coupling Resin (CAT#: Glyco-031CL)
Aminolink Coupling Resin is crosslinked 4% beaded agarose that has been activated with aldehyde groups to enable covalent immobilization of antibodies and other proteins through primary amines.
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Specifications
- Product Size
- 10 mL
50 mL
- Format
- Bottle(s)
- Column Type
- Affinity, Agarose Resin
- Immobilized Phase
- Aldehyde
- Contents & storage
- Store at 4°C.
Background
- Description
- Proteins and other molecules with primary amines can be covalently attached (immobilized) to Aminolink Resin to make chromatography columns for use in affinity purification. The aldehyde groups form stable secondary amine bonds with primary amines such as exist in the side chain of lysine (K) residues, which are generally abundant and readily accessible in proteins. Once a protein is immobilized, the prepared affinity resin can be used for a variety of batch and column affinity purification methods involving binding interactions with the immobilized protein. The resin and linkage are stable in most binding and elution conditions typically used in affinity chromatography, enabling prepared resin to be used for multiple rounds of affinity purification procedures.
The Aminolink Resin immobilization reaction involves spontaneous formation of Schiff base bonds between aldehydes and amines and their subsequent stabilization by incubation with a mild reductant. The entire coupling reaction, called reductive amination, occurs in 4 to 6 hours in simple non-amine buffers such as PBS. Coupling efficiency with antibodies and typical proteins is generally greater than 85%, resulting in 1 to 20 mg of immobilized protein per milliliter of resin.
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Recombinant Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-0402ZL | Human IgG1, kappa - Isotype Control Antibody | ELISA | |
| MRO-075-PMZ | Anti-C. difficile cell surface protein Polyclonal Antibody | WB, ELISA | Rabbit antibody |
| MRO-523-PMZ | Anti-Bacterial alkaline phosphatase Polyclonal Antibody (HRP) | ELISA | Rabbit antibody |
| MRO-623-RMZ | Recombinant Anti-P. aeruginosa Antibody (B11) | ELISA | Mouse antibody |
| MRO-776-RMZ | Recombinant Anti-Streptococcus equi subsp. equi Antibody (MAB212P) | ELISA | Mouse antibody |
Mouse Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| PABX-171 | Recombinant Mouse Anti-Shigella flexneri variant Y Antibody (SYA/J6) | WB, ELISA, FuncS | IgG |
| PABX-171-S (P) | Recombinant Mouse Anti-Shigella flexneri variant Y Antibody scFv Fragment (SYA/J6) | WB, ELISA, FuncS | scFv |
| PABX-171-F (E) | Recombinant Mouse Anti-Shigella flexneri variant Y Antibody Fab Fragment (SYA/J6) | WB, ELISA, FuncS | Fab |
MHC Tetramer for Cancer
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MHC-LC3377 | PE-B*14:02/Human (DRYRRIHSL) MHC Tetramer | FCM |
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For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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