Hi-Affi™ Rabbit Anti-ACPP Recombinant Antibody (clone DS43AB)

CAT#: MOR-0043

Recombinant Rabbit anti-ACPP monoclonal antibody for ICC, IHC-P, IP, WB, excellent for producing superior results. Hi-Affi™ antibodies are developed from different sources, such as B cells of immunized animals, hybridoma, phage display library. The application of recombinant technology avoids the traditional ascitic fluid approach, which achieves the antibody generation in a humane way with much larger scale, greater specificity and sensitivity, and not susceptible to cell-line drift or lot-to-lot variation.

Specifications

  • Immunogen
  • Synthetic peptide against human Prostatic Acid Phosphatase(P15309).
  • Host Species
  • Rabbit
  • Derivation
  • Phage display library screening
  • Type
  • Rabbit IgG
  • Species Reactivity
  • Human
  • Clone
  • DS43AB
  • Applications
  • ICC, IHC-P, IP, WB
  • Conjugate
  • Unconjugated

Product Property

  • Purification
  • Tissue culture supernatant
  • Purity
  • >95% as determined by analysis by SDS-PAGE
  • Format
  • Liquid
  • Storage
  • Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Target

  • Alternative Names
  • Acid Phosphatase, Prostate; Thiamine Monophosphatase; Ecto-5-Nucleotidase; 5-Nucleotidase; EC 3.1.3.2; TMPase; 5-NT; Prostatic Acid Phosphatase; Prostatic Acid Phosphotase; EC 3.1.3.5; ACP-3; ACP3; PAP
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Breast cancer biomarkers at key points during disease progression
High Affinity For Prostate Phosphatase
The Hi-Affi™ recombinant rabbit antibody (clone DS43AB) showed excellent affinity for Prostatic Acid Phosphatase (ACPP) in our experiments. We used it for IHC on paraffin-embedded sections and obtained clear, specific staining of the prostate epithelium. The recombinant technology provides better lot-to-lot consistency.
Breast cancer biomarkers at key points during disease progression
Clear Western Blot Bands
We also validated this antibody in Western Blot and Immunoprecipitation. It detected the target protein cleanly without non-specific bands. The sensitivity is high, allowing us to use lower concentrations of the antibody. It has been a valuable tool for our research into androgen-regulated enzymes.

Q&As

  1. How is this antibody produced?

    A: This Hi-Affi™ antibody (Clone DS43AB) is a Recombinant Rabbit monoclonal antibody generated via phage display library screening. This method allows for the selection of high-affinity binders and avoids the variability associated with traditional hybridoma or ascitic fluid production.

  2. What are the recommended applications for this ACPP antibody?

    A: This antibody is excellent for producing superior results in Immunocytochemistry (ICC), Immunohistochemistry on paraffin-embedded sections (IHC-P), Immunoprecipitation (IP), and Western Blot (WB). It targets Prostatic Acid Phosphatase (ACPP).

View the frequently asked questions answered by Creative Biolabs Support.

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Downloadable Resources

Download resources about recombinant antibody development and antibody engineering to boost your research.

Product Notes

This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production

See more details about Hi-Affi™ recombinant antibody benefits.

Datasheet

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COA

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For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.

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