Hi-Affi™ Rabbit Anti-ACSL4 Recombinant Antibody (clone DS45AB)
CAT#: MOR-0045
Recombinant Rabbit anti-ACSL4 monoclonal antibody for IHC-P, IP, WB, FC, ICC/IF, excellent for producing superior results. Hi-Affi™ antibodies are developed from different sources, such as B cells of immunized animals, hybridoma, phage display library. The application of recombinant technology avoids the traditional ascitic fluid approach, which achieves the antibody generation in a humane way with much larger scale, greater specificity and sensitivity, and not susceptible to cell-line drift or lot-to-lot variation.
Gene Expression
Subcellular Location
Figure 1 IF staining of human cell line U-251 MG
Immunofluorescent staining of human cell line U-251 MG shows localization to the Golgi apparatus.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/286/if_selected.jpg
Normal Tissue
Figure 2 IHC staining of human cerebral cortex
Immunohistochemical staining of human cerebral cortex shows strong cytoplasmic positivity in neuronal cells.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/5552/15785_B_8_5_selected.jpg
Normal Tissue
Figure 3 Cerebral cortex
Neuronal cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/5552/15785_B_8_5.jpg
Normal Tissue
Figure 4 Colon
Enterocytes - Mucosal lymphoid cells Staining: Medium Intensity: Moderate
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/5552/15785_A_7_3.jpg
Normal Tissue
Figure 5 Liver
Hepatocytes Staining: Low Intensity: Weak Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/5552/15785_A_8_4.jpg
Normal Tissue
Figure 6 Kidney
Collecting ducts Staining: Medium Intensity: Moderate Quantity: 75%-25% Distal tubules Staining: Medium Intensity: Strong Quantity: <25%
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/5552/15785_A_8_5.jpg
Normal Tissue
Figure 7 Testis
Leydig cells Staining: Low Intensity: Weak Quantity: 75%-25%
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/5552/15785_A_6_6.jpg
Normal Tissue
Figure 8 Lymph node
Germinal center cells Staining: Low Intensity: Moderate Quantity: <25% Location: Cytoplasmic/ membranous Non-germinal center cells Staining: Low Intensity: Moderate Quantity: <25% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/5552/15785_A_8_8.jpg
RNA Expression
Figure 9 RNA cell line category: Cell line enhanced (HMC-1)
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000068366-ACSL4
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Specifications
- Immunogen
- A synthetic peptide corresponding to residues in Human FACL4 was used as an immunogen. (The amino acid sequence is considered to be commercially sensitive).
- Host Species
- Rabbit
- Derivation
- Phage display library screening
- Type
- Rabbit IgG
- Species Reactivity
- Mouse, Rat, Human
- Clone
- DS45AB
- Applications
- IHC-P, IP, WB, FC, IF
- Conjugate
- Unconjugated
Product Property
- Purification
- Purified with Absolute Mag™ Protein A Agarose
- Purity
- >95% as determined by analysis by SDS-PAGE
- Format
- Liquid
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Target
- Alternative Names
- Acyl-CoA Synthetase Long-Chain Family Member 4; Fatty-Acid-Coenzyme A Ligase, Long-Chain 4; Long-Chain Fatty-Acid-Coenzyme A Ligase 4; Long-Chain Acyl-CoA Synthetase 4; Lignoceroyl-CoA Synthase; EC 6.2.1.3; LACS 4; FACL4; LACS4; ACS4; Mental Retardation, X-Linked 63; Mental Retardation, X-Linked 68; Acyl-CoA Synthetase 4; MRX63; MRX68
- Gene ID
- 2182
- UniProt ID
- O60488
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Downloadable Resources
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
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Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
See other products for "ACSL4"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS3-FY21 | Recombinant Rabbit Anti-ACSL4 Antibody (clone R07-4E3) | WB, IHC-P, IP | Rabbit IgG |
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