Hi-Affi™ Rabbit Anti-ACTN1 Recombinant Antibody (clone DS54AB) (CAT#: MOR-0054)

Name: Hi-Affi™ Rabbit Anti-ACTN1 Recombinant Antibody (clone DS54AB)
SKU: MOR-0054
Rating: 5 (1 reviews)

Recombinant Rabbit anti-ACTN1 monoclonal antibody for FC, ICC/IF, IP, WB, excellent for producing superior results. Hi-Affi™ antibodies are developed from different sources, such as B cells of immunized animals, hybridoma, phage display library. The application of recombinant technology avoids the traditional ascitic fluid approach, which achieves the antibody generation in a humane way with much larger scale, greater specificity and sensitivity, and not susceptible to cell-line drift or lot-to-lot variation.

Custom Production

We specialize in custom recombinant antibody production, offering seamless execution from provided sequences to high-quality antibody deliverables, ensuring optimal yield and purity.

We require custom production

Gene Expression
Datasheet
MSDS
COA

Subcellular Location

Figure 1 IF staining of human cell line U-2 OS
(Immunofluorescent staining of human cell line U-2 OS shows localization to plasma membrane & focal adhesion sites.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/4303/669_H5_2_selected.jpg

Figure 2 IF staining of human cell line A-431
(Immunofluorescent staining of human cell line A-431 shows localization to actin filaments.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/6035/if_selected.jpg

Normal Tissue

Figure 3 Testis
(Elongated or late spermatids Staining: Medium Intensity: Moderate Quantity:>75% Pachytene spermatocytes Staining: Medium Intensity: Moderate Quantity:>75% Round or early spermatids Staining: Medium Intensity: Moderate Quantity:>75%)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/6035/18297_A_6_6.jpg

Figure 4 Smooth muscle
(Smooth muscle cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/6035/18495_B_5_7.jpg

Figure 5 Lymph node
(Germinal center cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Non-germinal center cells Staining: High Intensity: Strong Quantity: 75%-25% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/6035/18297_A_7_8.jpg

Figure 6 Colon
(Endothelial cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Glandular cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous Peripheral nerve/ganglion Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/6035/18297_A_8_3.jpg

Figure 7 Liver
(Cholangiocytes Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/6035/18297_A_8_4.jpg

Figure 8 Kidney
(Cells in glomeruli Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous Cells in tubules Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/6035/18297_A_9_5.jpg

Figure 9 Cerebral cortex
(Endothelial cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Neuronal cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous Neuropil Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/6035/18495_B_8_5.jpg

RNA Expression

Figure 10 RNA cell line category: Low cell line specificity
(Cell lines ordered by descending RNA expression order)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000072110-ACTN1

Datasheet

MSDS

Certificate of Analysis Lookup
To download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number

Specifications

Immunogen
Synthetic peptide corresponding to residues on the C terminal of human alpha Actinin.

Host Species
Rabbit

Derivation
Phage display library screening

Type
Rabbit IgG

Species Reactivity
Human

Clone
DS54AB

Applications
FC, IF, IP, WB

Conjugate
Unconjugated

Product Property

Purification
Purified with Absolute Mag™ Protein A Agarose

Purity
>95% as determined by analysis by SDS-PAGE

Format
Liquid

Storage
Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Target

Alternative Names
Actinin, Alpha 1; Alpha-Actinin Cytoskeletal Isoform; F-Actin Cross-Linking Protein; Non-Muscle Alpha-Actinin-1; Actinin 1 Smooth Muscle; BDPLT15

Gene ID
87

UniProt ID
P12814

Product Notes

This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production

See more details about Hi-Affi™ recombinant antibody benefits.

Downloads

Download resources about recombinant antibody development and antibody engineering to boost your research.

See other products for "ACTN1"

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IgG Antibody Products Antibody Magnetic Beads IHC Kit and Antibody Products: Precision Tools for Immunohistochemistry

CAT	Product Name	Application	Type
MOB-3334z	Mouse Anti-ACTN1 Recombinant Antibody (clone 1H2)	WB, ELISA, FC	Mouse IgG2a, κ
MOB-0593MZ	Mouse Anti-ACTN1 Recombinant Antibody (clone 0.U.03)	IF, IHC, WB	Mouse IgG1
MRO-1640-CN	Rabbit Anti-ACTN1 Polyclonal Antibody (MRO-1640-CN)	WB, IF, IHC, FC	Rabbit IgG
VS3-QX21	Mouse Anti-ACTN1 Recombinant Antibody (clone 1A5-7D1-9H10)	IHC	Mouse IgG1
VS4-WK17	Mouse Anti-ACTN1 Recombinant Antibody (VS4-WK17)	ELISA, IHC	Mouse IgG

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For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.

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