Rat Anti-AChR Recombinant Antibody (clone mAb198)
CAT#: PABZ-007
Recombinant Rat Antibody (mAb198) is capable of binding to AChR. This antibody binds to a region on the extracellular surface of the a subunit of the AChR named the main immunogenic region (MIR).
Published Data
Figure 1 Immunoblots of short fusion proteins.
Equivalent amounts of fusion proteins Tax2 (left), X8 (center) and CaX2 (right) were electrophoresed and transferred by diffusion as described (8). Replicates were probed with control mAb 118 (anti+ chain of Torpedo nAChR), anti-MIR mAbs 42, 37, 6, and 198, and a-BTX. The molecular masses of the fusion proteins are approximately 30 kDa; CaX2 migrates with an apparent mass of 20 kDa.
Barkas, T., Gabriel, J. M., Mauron, A., Hughes, G. J., Roth, B., Alliod, C., ... & Ballivet, M. (1988). Monoclonal antibodies to the main immunogenic region of the nicotinic acetylcholine receptor bind to residues 61-76 of the alpha subunit. Journal of Biological Chemistry, 263(12), 5916-5920.
Figure 2 ELISA tests using X4 and X8.
Binding of control mAb 118 (boxes) and anti-MIR mAbs 6 (open circles), 37 (open triangles), 42 (closed triangles), and 198 (closed circles) to X4 (left) and X8 (right) was tested by ELISA as described under "Materials and Methods." Results shown are the mean and standard error or three separate determinations.
Barkas, T., Gabriel, J. M., Mauron, A., Hughes, G. J., Roth, B., Alliod, C., ... & Ballivet, M. (1988). Monoclonal antibodies to the main immunogenic region of the nicotinic acetylcholine receptor bind to residues 61-76 of the alpha subunit. Journal of Biological Chemistry, 263(12), 5916-5920.
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Specifications
- Immunogen
- Human Acetylcholine receptor
- Host Species
- Rat
- Derivation
- Rat
- Type
- Rat IgG
- Specificity
- AChR
- Species Reactivity
- Human
- Clone
- mAb198
- Applications
- WB, ELISA
Product Property
- Purity
- >95% as determined by analysis by SDS-PAGE
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- PBS
- Preservative
- No preservatives
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Target
- Alternative Names
- Acetylcholine receptor; AChR
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Easy to Use
The handling instructions and datasheet that were included made it simple to use this antibody in our studies and prevented any problems.
Consistent Quality
This antibody has been a constant order for us over a number of batches, so we know it will work as intended in experiments.
Robust Performance
This antibody performs well under a variety of experimental settings, making it a dependable option for studies.
Q&As
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What common pitfalls should I avoid in antibody-based assays?
A: Common pitfalls include improper sample preparation, incorrect antibody dilutions, insufficient blocking, and failure to optimize detection methods. Attention to these details helps ensure reliable results.
-
How do I determine the reactivity of this antibody with other species?
A: Cross-reactivity information can often be found in the product datasheet. Additionally, you can perform preliminary tests using samples from different species.
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What methods can optimize antibody-antigen binding?
A: Optimize incubation times, temperatures, and concentrations. Use enhanced blocking agents and consider secondary antibody amplification techniques to improve binding efficiency.
View the frequently asked questions answered by Creative Biolabs Support.
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Downloadable Resources
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
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- CAT
- Product Name
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| CAT | Product Name | Application | Type |
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| MHH-33 | Recombinant Human Anti-AChR Antibody | ELISA, WB, IP, FuncS | IgG |
| CAT | Product Name | Application | Type |
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| MHH-33-F(E) | Recombinant Human Anti-AChR Antibody Fab Fragment | IF, ELISA, FuncS | Fab |
| CAT | Product Name | Application | Type |
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| MHH-33-S(P) | Recombinant Human Anti-AChR Antibody scFv Fragment | ELISA, WB, FuncS | scFv |
| CAT | Product Name | Application | Type |
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| PABC-007 | Rat Anti-AChR Recombinant Antibody (clone mAb192) | WB, FuncS | Rat IgG2b, κ |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| PFBC-007 | Rat Anti-AChR Recombinant Antibody (clone mAb192); Fab Fragment | WB, FuncS | Rat IgG2b, κ |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| PFBZ-007 | Rat Anti-AChR Recombinant Antibody (clone mAb198); Fab Fragment | WB, ELISA | Rat IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| PSBC-007 | Rat Anti-AChR Recombinant Antibody (clone mAb192); scFv Fragment | WB, FuncS | Rat scFv |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| PSBZ-007 | Rat Anti-AChR Recombinant Antibody (clone mAb198); scFv Fragment | WB, ELISA | Rat scFv |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-0636CLV | Anti-Human AChR Recombinant Antibody (P90-131) | WB | Fully Human antibody |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-0637CLV | Anti-Human AChR Recombinant Antibody (P90-65) | RIA, IF | Fully Human antibody |
| CAT | Product Name | Application | Type |
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| TAB-0638CLV | Human Anti-AChR Recombinant Antibody (TAB-0638CLV) | Dot, FuncS, IF | Human IgG1 |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-0639CLV | Human Anti-AChR Recombinant Antibody (TAB-0639CLV) | RIA, IF | Human IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-0638CLV-F(E) | Human Anti-AChR Recombinant Antibody; Fab Fragment (TAB-0638CLV-F(E)) | ELISA | Human Fab |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-082CQ | Mouse Anti-AChR Recombinant Antibody (clone mAb192) | ELISA, WB, Block | Mouse IgG2a, κ |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| FAMAB-0223YC | Rat Anti-AChR Recombinant Antibody (clone mAb 192) | FuncS | Rat IgG2b |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| FAMAB-0223-YC-S(P) | Rat Anti-AChR Recombinant Antibody (clone mAb 192); scFv Fragment | FuncS | Rat scFv |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| FAMAB-0223-YC-F(E) | Rat Anti-AChR Recombinant Antibody (clone mAb 192); Fab Fragment | FuncS | Rat Fab |
| CAT | Product Name | Application | Type |
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