Rat Anti-AChR Recombinant Antibody (clone mAb198) (CAT#: PABZ-007)

Figure 1 Immunoblots of short fusion proteins.

Equivalent amounts of fusion proteins Tax2 (left), X8 (center) and CaX2 (right) were electrophoresed and transferred by diffusion as described (8). Replicates were probed with control mAb 118 (anti+ chain of Torpedo nAChR), anti-MIR mAbs 42, 37, 6, and 198, and a-BTX. The molecular masses of the fusion proteins are approximately 30 kDa; CaX2 migrates with an apparent mass of 20 kDa.

Barkas, T., Gabriel, J. M., Mauron, A., Hughes, G. J., Roth, B., Alliod, C., ... & Ballivet, M. (1988). Monoclonal antibodies to the main immunogenic region of the nicotinic acetylcholine receptor bind to residues 61-76 of the alpha subunit. Journal of Biological Chemistry, 263(12), 5916-5920.

Figure 2 ELISA tests using X4 and X8.

Binding of control mAb 118 (boxes) and anti-MIR mAbs 6 (open circles), 37 (open triangles), 42 (closed triangles), and 198 (closed circles) to X4 (left) and X8 (right) was tested by ELISA as described under "Materials and Methods." Results shown are the mean and standard error or three separate determinations.

Barkas, T., Gabriel, J. M., Mauron, A., Hughes, G. J., Roth, B., Alliod, C., ... & Ballivet, M. (1988). Monoclonal antibodies to the main immunogenic region of the nicotinic acetylcholine receptor bind to residues 61-76 of the alpha subunit. Journal of Biological Chemistry, 263(12), 5916-5920.