Human Anti-AGR2 Recombinant Antibody (TAB-0708CLV) (CAT#: TAB-0708CLV)

This product is a recombinant Human antibody that recognizes AGR2. The antibody was expressed in mammalian cells with chemically defined culture media and was purified by affinity chromatography.


Specific Inquiry
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DB

Figure 1 Anti-AGR2 Recombinant Antibody (TAB-0708CLV) in DB

Figure 1 Anti-AGR2 Recombinant Antibody (TAB-0708CLV) in DB

Dot Blot analysis of TAB-0708CLV was performed by coating with human AGR2 protein (His tag).
TAB-0708CLV incubation concentration: 2 μg/mL.
HRP-conjugated goat anti-Human IgG as a secondary antibody (1: 2000)

WB

Figure 2 Anti-AGR2 Recombinant Antibody (TAB-0708CLV) in WB

Figure 2 Anti-AGR2 Recombinant Antibody (TAB-0708CLV) in WB

Western blot analysis of TAB-0708CLV was performed by loading human AGR2 protein (His tag) onto a 12% Tris-HCl polyacrylamide gel. Proteins were transferred to a CN membrane and blocked with 5% skim milk for at least one hour. Membranes were probed with TAB-0708CLV and HRP goat anti-Human IgG as a secondary antibody (1: 2000). Chemiluminescent detection was performed.

Lane 1: Reduced antigen (0.1 μg)
Lane 2: Reduced antigen (0.3 μg)
Lane 3: Reduced antigen (0.6 μg)

ELISA

Figure 3 Anti-AGR2 Recombinant Antibody (TAB-0708CLV) in ELISA

Figure 3 Anti-AGR2 Recombinant Antibody (TAB-0708CLV) in ELISA

ELISA analysis of TAB-0708CLV was performed by coating with human AGR2 protein (His tag). Then blocked with BSA and incubated with TAB-0708CLV. The HRP-conjugated goat anti-Human IgG as a secondary antibody (1: 2000). Detection was performed using TMB substrate and stopped with sulfuric acid. The absorbances were read on a spectrophotometer at 450 nm.

DB

Figure 4 Anti-AGR2 Recombinant Antibody (TAB-0708CLV) in DB

Figure 4 Anti-AGR2 Recombinant Antibody (TAB-0708CLV) in DB

Dot Blot analysis of TAB-0708CLV was performed by coating with mouse AGR2 protein (His tag).
TAB-0708CLV incubation concentration: 2 μg/mL.
HRP-conjugated goat anti-Human IgG as a secondary antibody (1: 2000)

WB

Figure 5 Anti-AGR2 Recombinant Antibody (TAB-0708CLV) in WB

Figure 5 Anti-AGR2 Recombinant Antibody (TAB-0708CLV) in WB

Western blot analysis of TAB-0708CLV was performed by loading mouse AGR2 protein (His tag) onto a 12% Tris-HCl polyacrylamide gel. Proteins were transferred to a CN membrane and blocked with 5% skim milk for at least one hour. Membranes were probed with TAB-0708CLV and HRP goat anti-Human IgG as a secondary antibody (1: 2000). Chemiluminescent detection was performed.

Lane 1: Reduced antigen (0.1 μg)
Lane 2: Reduced antigen (0.3 μg)
Lane 3: Reduced antigen (0.6 μg)

ELISA

Figure 6 Anti-AGR2 Recombinant Antibody (TAB-0708CLV) in ELISA

Figure 6 Anti-AGR2 Recombinant Antibody (TAB-0708CLV) in ELISA

ELISA analysis of TAB-0708CLV was performed by coating with mouse AGR2 protein (His tag). Then blocked with BSA and incubated with TAB-0708CLV. The HRP-conjugated goat anti-Human IgG as a secondary antibody (1: 2000). Detection was performed using TMB substrate and stopped with sulfuric acid. The absorbances were read on a spectrophotometer at 450 nm.


Specifications

  • Host Species
  • Human
  • Type
  • Humanized IgG1, κ
  • Specificity
  • Human AGR2
  • Species Reactivity
  • Cross-react with murine AGR2
  • Applications
  • Inhib, FC
  • Related Disease
  • Tumor

Product Property

  • Purity
  • >95% as determined by SDS-PAGE
  • Concentration
  • Please refer to the vial label for the specific concentration.
  • Buffer
  • PBS
  • Preservative
  • No preservatives
  • Storage
  • Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Target

  • Alternative Names
  • AGR2; anterior gradient 2, protein disulphide isomerase family member; AG2; GOB-4; HAG-2; XAG-2; PDIA17; HEL-S-11

Product Notes

This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production

See more details about Hi-Affi™ recombinant antibody benefits.

Downloads

Download resources about recombinant antibody development and antibody engineering to boost your research.

See other products for "AGR2"

Single-domain Antibody

CAT Product Name Application Type
NAB-130-VHH Recombinant Anti-Human AGR2 VHH Single Domain Antibody IHC, IP, FC, Neut, FUNC Llama VHH

Rabbit Monoclonal Antibody

CAT Product Name Application Type
MOR-0102 Hi-Affi™ Rabbit Anti-AGR2 Recombinant Antibody (clone DS102AB) FC, ICC, IHC-P, IP, WB Rabbit IgG

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For Research Use Only. Not For Clinical Use.

For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.

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