Mouse Anti-AHLs Recombinant Antibody (clone RS2-1G9) (CAT#: PABL-007)
Recombinant Mouse Antibody (RS2-1G9) is capable of binding to AHLs.
Specific Inquiry
WB
Figure 1 Western blot analysis of BMDM protection from cytotoxic effects of 3-oxo-C12-HSL by mAb RS2-1G9.
RS2-1G9 prevents p38 activation and PARP cleavage in BMDM in response to 3-oxo-C12-HSL.
Kaufmann, G. F. , Park, J. , Mee, J. M. , Ulevitch, R. J. , & Janda, K. D.. (2008). The quorum quenching antibody rs2-1g9 protects macrophages from the cytotoxic effects of the pseudomonas aeruginosa quorum sensing signalling molecule n-3-oxo-dodecanoyl-homoserine lactone. Molecular Immunology, 45(9), 2710-2714.
Inhib
Figure 2 Inhibition of pyocyanin production in P. aeruginosa PAO (wildtype, filled columns) and in PAO-JM2 (open columns) by mAb RS2-1G9.
Kaufmann, G. F., Sartorio, R., Lee, S. H., Mee, J. M., Altobell, L. J., Kujawa, D. P., ... & Janda, K. D. (2006). Antibody interference with N-acyl homoserine lactone-mediated bacterial quorum sensing. Journal of the American Chemical Society, 128(9), 2802-2803.
Specifications
- Immunogen
- The details of the immunogen for this antibody are not available.
- Host Species
- Mouse
- Derivation
- Mouse
- Type
- Mouse IgG1, λ
- Specificity
- AHLs
- Clone
- RS2-1G9
- Applications
- WB, ELISA, IP, Inhib, FuncS
Product Property
- Purity
- >95% as determined by analysis by SDS-PAGE
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- PBS
- Preservative
- No preservatives
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- The antibody RS2-1G9 has been reported in applications of Western Blot, Enzyme-linked Immunosorbent Assay, Immunoprecipitation, Inhibition, Functional Assay. It's recommended that the optimal antibody concentration, dilution, incubition time etc. be carefully titrated in specific assays.
Target
- Alternative Names
- N-Acyl homoserine lactones; AHLs; N-AHLs
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
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Recombinant Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-46 | Recombinant Anti-AHLs Antibody | WB, Neut, FuncS | IgG |
| MHH-46 | Recombinant Human Anti-AHLs Antibody | RIA, IP, FuncS | IgG |
Fab Fragment Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-46-F(E) | Recombinant Anti-AHLs Antibody Fab Fragment | IP, IF, FuncS | Fab |
| MHH-46-F(E) | Recombinant Human Anti-AHLs Antibody Fab Fragment | FC, Neut, Funcs | Fab |
| PFBL-007 | Mouse Anti-AHLs Recombinant Antibody (clone RS2-1G9); Fab Fragment | WB, ELISA, IP, FuncS | Mouse Fab |
scFv Fragment Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-46-S(P) | Recombinant Anti-AHLs Antibody scFv Fragment | WB, FC, FuncS | scFv |
| MHH-46-S(P) | Recombinant Human Anti-AHLs Antibody scFv Fragment | WB, IP, FuncS | scFv |
| PSBL-007 | Mouse Anti-AHLs Recombinant Antibody (clone RS2-1G9); scFv Fragment | WB, ELISA, IP, FuncS | Mouse scFv |
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For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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