Hi-Affi™ Rabbit Anti-AKT1S1 Recombinant Antibody (clone TH185DS) (CAT#: MOR-4671)
Recombinant Rabbit anti-AKT1S1 monoclonal antibody for WB, IF, ICC, FCM, excellent for producing superior results. Hi-Affi™ antibodies are developed from different sources, such as B cells of immunized animals, hybridoma, phage display library. The application of recombinant technology avoids the traditional ascitic fluid approach, which achieves the antibody generation in a humane way with much larger scale, greater specificity and sensitivity, and not susceptible to cell-line drift or lot-to-lot variation.
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Subcellular Location
Normal Tissue
RNA Expression
Figure 1 IF staining of human cell line HEK 293
(Immunofluorescent staining of human cell line HEK 293 shows localization to cytosol.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/43590/if_selected.jpg
(Immunofluorescent staining of human cell line HEK 293 shows localization to cytosol.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/43590/if_selected.jpg
Figure 2 IF staining of human cell line U-2 OS
(Immunofluorescent staining of human cell line U-2 OS shows localization to cytosol.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/21903/if_selected.jpg
(Immunofluorescent staining of human cell line U-2 OS shows localization to cytosol.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/21903/if_selected.jpg
Figure 3 IHC staining of human stomach
(Immunohistochemical staining of human stomach shows cytoplasmic and nuclear positivity in glandular cells.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/64427/ihc_selected.jpg
(Immunohistochemical staining of human stomach shows cytoplasmic and nuclear positivity in glandular cells.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/64427/ihc_selected.jpg
Figure 4 IHC staining of human pancreas
(Immunohistochemical staining of human pancreas shows strong cytoplasmic positivity in exocrine and islet cells.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/21903/ihc_selected.jpg
(Immunohistochemical staining of human pancreas shows strong cytoplasmic positivity in exocrine and islet cells.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/21903/ihc_selected.jpg
Figure 5 Cerebral cortex
(Neuronal cells Staining: Low Intensity: Moderate Quantity: <25% Location: Cytoplasmic/ membranous nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/64427/150348_B_7_5.jpg
(Neuronal cells Staining: Low Intensity: Moderate Quantity: <25% Location: Cytoplasmic/ membranous nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/64427/150348_B_7_5.jpg
Figure 6 Colon
(Endothelial cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Nuclear Glandular cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/64427/150348_A_8_3.jpg
(Endothelial cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Nuclear Glandular cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/64427/150348_A_8_3.jpg
Figure 7 Testis
(Cells in seminiferous ducts Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous nuclear Leydig cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/64427/150348_A_6_6.jpg
(Cells in seminiferous ducts Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous nuclear Leydig cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/64427/150348_A_6_6.jpg
Figure 8 RNA cell line category: Low cell line specificity
(Cell lines ordered by descending RNA expression order)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000204673-AKT1S1
(Cell lines ordered by descending RNA expression order)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000204673-AKT1S1
Specifications
- Immunogen
- Phosphopeptide corresponding to amino acids 241-251 of human PRAS40
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Species Reactivity
- Human, Mouse
- Clone
- TH185DS
- Applications
- WB, IF, ICC, FC
- Conjugate
- Unconjugated
Product Property
- Purification
- Purified with Absolute Mag™ Protein A Agarose
- Purity
- >95% as determined by analysis by SDS-PAGE
- Format
- Liquid
- Buffer
- PBS
- Preservative
- 0.09% sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Target
- Alternative Names
- AKT1 Substrate 1; 40 KDa Proline-Rich AKT Substrate; AKT1 Substrate 1 (Proline-Rich); AKT1 Substrate 1 (Proline Rich); PRAS40; Proline-Rich Akt Substrate; 40 KDa; Lobe
- Gene ID
- 84335
- UniProt ID
- Q96B36
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
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Rabbit Monoclonal Antibodies
IgG Antibodies
IHC Kits & Antibodies
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-0127 | Hi-Affi™ Rabbit Anti-AKT1S1 Recombinant Antibody (clone DS127AB) | WB, IP | Rabbit IgG |
| MOR-4529 | Hi-Affi™ Rabbit Anti-AKT1S1 Recombinant Antibody (clone TH37DS) | WB, IF, ICC | Rabbit IgG |
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