Recombinant Anti-Human, mouse, and rat ALB IgNAR Antibody (PNBL-002)
CAT#: PNBL-002
Anti-Human, mouse, and rat ALB IgNAR Antibody is a recombinant protein produced in E. coli.
Published Data
Gene Expression
ELISA
Figure 1 ELISA determination of lead v1.10 and v2.4 humanized E06 clones.
Steven, J., Müller, M. R., Carvalho, M. F., Ubah, O. C., Kovaleva, M., Donohoe, G., ... & Barelle, C. J. (2017). In vitro maturation of a humanized shark Vnar domain to improve its biophysical properties to facilitate clinical development. Frontiers in immunology, 8, 1361.
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Subcellular Location and Protein Expression
Figure 1 IF staining of human cell line Hep G2
Immunofluorescent staining of human cell line Hep G2 shows localization to endoplasmic reticulum & the Golgi apparatus.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/31024/763_G10_1_selected.jpg
Subcellular Location and Protein Expression
Figure 2 IHC staining of human fallopian tube
Immunohistochemical staining of human fallopian tube shows distinct positivity in plasma.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/6262/17728_A_9_7_selected.jpg
Normal Tissue
Figure 3 Cerebral cortex
Neuronal cells
Staining:Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/31024/128566_B_7_5.jpg
Normal Tissue
Figure 4 Colon
Endothelial cells
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/31024/128566_A_7_3.jpg
Normal Tissue
Figure 5 Kidney
Bowman's capsule
Staining:Medium
Intensity: Moderate
Quantity:>75%
Proximal tubules (microvilli)
Staining:Medium
Intensity: Moderate
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/31024/128566_A_7_5.jpg
Normal Tissue
Figure 6 Testis
Cells in seminiferous ducts
Staining:Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous nuclear
Leydig cells
Staining:Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/31024/128566_A_6_6.jpg
RNA Expression
Figure 7 RNA cell line category: Cell line enriched (Hep G2)
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000163631-ALB
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Specifications
- Immunogen
- Human albumin
- Host Species
- Shark
- Derivation
- Shark
- Type
- Shark IgNAR
- Species Reactivity
- Human, mouse, and rat
- Clone
- PNBL-002
- Applications
- ELISA
Product Property
- Purity
- >95% by SDS-PAGE and HPLC analysis
- Storage
- Store the antibody (in aliquots) at -20°C. Avoid repeated freezing and thawing of samples.
Applications
- Application Notes
- This antibody has been reported for use in Enzyme-linked Immunosorbent Assay. It's recommended that the optimal antibody concentration, dilution, incubition time, etc. should be carefully titrated in specific assays.
Target
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Customer Review
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Suitable for ELISA
I used this antibody in an ELISA setup and it delivered accurate and specific readings. It's a reliable antibody for quantitative assays.
Sturdy and Steady
When used in multiplex tests with other human antibodies, this antibody performs admirably and exhibits no issues with cross-reactivity.
Excellent for Multiplex Tests
Our studies with this antibody produced a very strong signal, which accelerated the collection and analysis of data.
Q&As
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How should I carry out an antibody titration to get the best results?
A: A series of dilutions between 1:50 and 1:5000 should be started. To find the best working dilution for your particular situation, evaluate the binding efficiency at each concentration.
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Which secondary antibodies should I use in conjunction with this product?
A: Anti-human IgG tagged with enzymes like HRP or fluorophores like FITC are advised, depending on your preferred method of detection. Verify that it works with the way your experiment is set up.
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Does this antibody have any specific handling guidelines?
A: Treat the antibody with care, and do not mix it vigorously. Before using, give it a quick centrifugation to prevent any possible material loss. To avoid degradation, keep stored in aliquots.
View the frequently asked questions answered by Creative Biolabs Support.
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Downloadable Resources
Download resources about recombinant antibody development and antibody engineering to boost your research.
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
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