How should I carry out an antibody titration to get the best results?

A series of dilutions between 1:50 and 1:5000 should be started. To find the best working dilution for your particular situation, evaluate the binding efficiency at each concentration.

Which secondary antibodies should I use in conjunction with this product?

Anti-human IgG tagged with enzymes like HRP or fluorophores like FITC are advised, depending on your preferred method of detection. Verify that it works with the way your experiment is set up.

Does this antibody have any specific handling guidelines?

Treat the antibody with care, and do not mix it vigorously. Before using, give it a quick centrifugation to prevent any possible material loss. To avoid degradation, keep stored in aliquots.

Recombinant Anti-Human, mouse, and rat ALB IgNAR Antibody (PNBL-002) (CAT#: PNBL-002)

Anti-Human, mouse, and rat ALB IgNAR Antibody is a recombinant protein produced in E. coli.

Custom Production

We specialize in custom recombinant antibody production, offering seamless execution from provided sequences to high-quality antibody deliverables, ensuring optimal yield and purity.

We require custom production

Published Data
Gene Expression
Datasheet
MSDS
COA

ELISA

Figure 1 ELISA determination of lead v1.10 and v2.4 humanized E06 clones.

Figure 1 ELISA determination of lead v1.10 and v2.4 humanized E06 clones.

Steven, J., Müller, M. R., Carvalho, M. F., Ubah, O. C., Kovaleva, M., Donohoe, G., ... & Barelle, C. J. (2017). In vitro maturation of a humanized shark Vnar domain to improve its biophysical properties to facilitate clinical development. Frontiers in immunology, 8, 1361.

Subcellular Location and Protein Expression

Figure 1 IF staining of human cell line Hep G2
(Immunofluorescent staining of human cell line Hep G2 shows localization to endoplasmic reticulum & the Golgi apparatus.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/31024/763_G10_1_selected.jpg

Figure 2 IHC staining of human fallopian tube
(Immunohistochemical staining of human fallopian tube shows distinct positivity in plasma.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/6262/17728_A_9_7_selected.jpg

Normal Tissue

Figure 3 Cerebral cortex
(Neuronal cells
Staining:Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/31024/128566_B_7_5.jpg

Figure 4 Colon
(Endothelial cells
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/31024/128566_A_7_3.jpg

Figure 5 Kidney
(Bowman's capsule
Staining:Medium
Intensity: Moderate
Quantity:>75%
Proximal tubules (microvilli)
Staining:Medium
Intensity: Moderate)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/31024/128566_A_7_5.jpg

Figure 6 Testis
(Cells in seminiferous ducts
Staining:Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous nuclear
Leydig cells
Staining:Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/31024/128566_A_6_6.jpg

RNA Expression

Figure 7 RNA cell line category: Cell line enriched (Hep G2)
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000163631-ALB

Datasheet

MSDS

Certificate of Analysis Lookup
To download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number

Specifications

Immunogen
Human albumin

Host Species
Shark

Derivation
Shark

Type
Shark IgNAR

Species Reactivity
Human, mouse, and rat

Clone
PNBL-002

Applications
ELISA

Product Property

Purity
>95% by SDS-PAGE and HPLC analysis

Storage
Store the antibody (in aliquots) at -20°C. Avoid repeated freezing and thawing of samples.

Applications

Application Notes
This antibody has been reported for use in Enzyme-linked Immunosorbent Assay. It's recommended that the optimal antibody concentration, dilution, incubition time, etc. should be carefully titrated in specific assays.

Target

Alternative Names
FDAH; ANALBA; PRO0883; PRO0903; PRO1341

Gene ID
213

UniProt ID
P02768

Product Notes

This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production

See more details about Hi-Affi™ recombinant antibody benefits.

Downloads

Download resources about recombinant antibody development and antibody engineering to boost your research.

See other products for "ALB"

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CAT	Product Name	Application	Type
NAB-81-sdAb	Recombinant Anti-human ALB VHH Single Domain Antibody	IHC, ICC, IP, ChiP, Neut	Llama VHH
PABX-001-VHH	Recombinant Anti-Human Albumins VHH Single Domain Antibody (PABX-001-VHH)	ELISA	Shark VHH
NBD-0046-YJ-VHH	Camelid Anti-ALB Recombinant Single Domain Antibody (NBD-0046-YJ-VHH)	ELISA, FC	Camelid VHH

CAT	Product Name	Application	Type
TAB-0511CL-S(P)	Human Anti-ALB Recombinant Antibody scFv Fragment (TAB-0511CL-S(P))	ELISA	Humanized Antibody
TAB-0512CL-S(P)	Human Anti-ALB Recombinant Antibody scFv Fragment (TAB-0512CL-S(P))	ELISA	Humanized Antibody
TAB-0513CL-S(P)	Human Anti-ALB Recombinant Antibody scFv Fragment (TAB-0513CL-S(P))	ELISA	Humanized Antibody
TAB-0514CL-S(P)	Human Anti-ALB Recombinant Antibody scFv Fragment (TAB-0514CL-S(P))	ELISA	Humanized Antibody
TAB-0515CL-S(P)	Human Anti-ALB Recombinant Antibody scFv Fragment (TAB-0515CL-S(P))	ELISA	Humanized Antibody

CAT	Product Name	Application	Type
TAB-431CT-S(P)	Anti-Human Serum Albumin Recombinant Antibody scFv Fragment (DOM7r31)	WB, ELISA	Human antibody
TAB-432CT-S(P)	Anti-Human Serum Albumin Recombinant Antibody scFv Fragment (DOM7r36)	WB, ELISA	Human antibody
TAB-431CT-F(E)	Anti-Human Serum Albumin Recombinant Antibody Fab Fragment (DOM7r31)	WB, ELISA	Human antibody
TAB-432CT-F(E)	Anti-Human Serum Albumin Recombinant Antibody Fab Fragment (DOM7r36)	WB, ELISA	Human antibody
TAB-433CT-F(E)	Anti-Human Serum Albumin Recombinant Antibody Fab Fragment (DOM7r92)	WB, ELISA	Human antibody

CAT	Product Name	Application	Type
MOB-0579MZ	Mouse Anti-ALB Recombinant Antibody (clone LU43)	ELISA, WB	Mouse IgG1
MRO-1633-CN	Rabbit Anti-ALB Polyclonal Antibody (MRO-1633-CN)	WB, IF, IHC, FC	Rabbit IgG
VS3-FY2601	Mouse Anti-ALB Recombinant Antibody (VS3-FY2601)	WB	Mouse IgG
VS7-HM73	Mouse Anti-ALB Recombinant Antibody (clone CBL028HM)	WB, ELISA	Mouse IgG
VS7-HM74	Mouse Anti-ALB Recombinant Antibody (clone CBL029HM)	WB	Mouse IgG

CAT	Product Name	Application	Type
BRD-0672MZ	Chicken Anti-Albumin Polyclonal IgY	WB	Chicken antibody

CAT	Product Name	Application	Type
MOR-0132	Hi-Affi™ Rabbit Anti-ALB Recombinant Antibody (clone DS132AB)	WB, IHC, FC	Rabbit IgG
MOR-0133	Hi-Affi™ Rabbit Anti-ALB Recombinant Antibody (clone DS133AB)	ELISA, WB	Rabbit IgG

CAT	Product Name	Application	Type
HPAB-0663-WJ-F(E)	Human Anti-ALB Recombinant Antibody; Fab Fragment (HPAB-0663-WJ-F(E))	ELISA	Humanized Fab
PFBS-0046	Rabbit Anti-ALB Recombinant Antibody; Fab Fragment (PFBS-0046)	ELISA, FuncS	Rabbit Fab

CAT	Product Name	Application	Type
HPAB-0663-WJ-S(P)	Human Anti-ALB Recombinant Antibody; scFv Fragment (HPAB-0663-WJ-S(P))	ELISA	Human scFv
PSBS-0046	Rabbit Anti-ALB Recombinant Antibody; scFv Fragment (PSBS-0046)	ELISA, FuncS	Rabbit scFv

CAT	Product Name	Application	Type
VS-0724-YC827	AbPlus™ Anti-ALB Magnetic Beads (VS-0724-YC827)	IP, Protein Purification

CAT	Product Name	Application	Type
VS-0125-FY116	Human Anti-ALB scFv-Fc Chimera (VS-0125-FY116)	ELISA, FC, FuncS	Human IgG1, scFv-Fc

CAT	Product Name	Application	Type
VS-0325-XY86	Anti-ALB Immunohistochemistry Kit	IHC

Customer Reviews and Q&As

Customer Review Q&As

Suitable for ELISA

I used this antibody in an ELISA setup and it delivered accurate and specific readings. It's a reliable antibody for quantitative assays.

Sturdy and Steady

When used in multiplex tests with other human antibodies, this antibody performs admirably and exhibits no issues with cross-reactivity.

Excellent for Multiplex Tests

Our studies with this antibody produced a very strong signal, which accelerated the collection and analysis of data.

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For Research Use Only. Not For Clinical Use.

For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.

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