Human Anti-ANG Recombinant Antibody (clone 26-2F)
CAT#: PABL-008
Recombinant Human Antibody (26-2F) is capable of binding to ANG, expressed in HEK 293 cells. Expressed as the combination of a heavy chain (HC) containing VH from anti-ANG mAb and CH1-3 region of human IgG1 and a light chain (LC) encoding VL from anti-ANG mAb and CL of human kappa light chain. Exists as a disulfide linked dimer of the HC and LC hetero-dimer under non-reducing condition.
Published Data
Gene Expression
IHC
Figure 1 IHC detection of human and mouse ANG in normal and cancer tissues.
Human ANG was detected by 26-2F, Mouse ANG was detected by an affinity-purified polyclonal antibody.
Li, S., & Hu, G. F. (2010). Angiogenin-mediated rRNA transcription in cancer and neurodegeneration. International journal of biochemistry and molecular biology, 1(1), 26.
FuncS
Figure 2 Survivor functions showing prevention of HT-29 tumor growth in athymic mice by treatment with Ang antagonists.
On day 0 HT-29 cells (1.25 X 10⁵ per mouse) are mixed with PBS or the Ang antagonist and injected s.c. Daily injections of PBS or the Ang antagonist are then given for the next 35 days. (A) …, PBS (n = 145); —, mAb 26-2F (60 ,ug; n = 20); - -, mAb 36u (60 μg; n = 41); ---, mAb 26-2F + mAb 36u (30 μg of each; n = 47). (B)..., PBS (n = 145); -, bovine actin (18 μg; n = 28).
Olson, K. A., Fett, J. W., French, T. C., Key, M. E., & Vallee, B. L. (1995). Angiogenin antagonists prevent tumor growth in vivo. Proceedings of the National Academy of Sciences, 92(2), 442-446.
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Subcellular Location
Figure 1 IF staining of human cell line Hep G2
Immunofluorescent staining of human cell line Hep G2 shows localization to nucleoli rim & mitotic chromosome.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/55896/1144_B11_1_selected.jpg
Subcellular Location
Figure 2 IF staining of human cell line A-431
Immunofluorescent staining of human cell line A-431 shows localization to nucleoli rim & mitotic chromosome.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/55896/893_E9_1_red_green.jpg
Subcellular Location
Figure 3 IF staining of human cell line U-2 OS
Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoli rim & mitotic chromosome.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/55896/895_E9_1_red_green.jpg
RNA Expression
Figure 4 RNA cell line category: Group enriched (Hep G2, OE19)
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000214274-ANG
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Specifications
- Immunogen
- Human angiogenin
- Host Species
- Human
- Derivation
- Human
- Type
- Human IgG1, κ
- Specificity
- Human ANG
- Species Reactivity
- Human
- Clone
- 26-2F
- Applications
- WB, IHC, IF, FuncS
Product Property
- Purity
- >95% as determined by analysis by SDS-PAGE
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- PBS
- Preservative
- No preservatives
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- The antibody 26-2F has been reported in applications of Western Blot, Immunohistochemistry, Immunofluorescence, Functional Assay. It's recommended that the optimal antibody concentration, dilution, incubition time etc. be carefully titrated in specific assays.
Target
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
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MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
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Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
See other products for "Clone 26-2F"
- CAT
- Product Name
See other products for "ANG"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-64 | Recombinant Anti-human ANG Antibody | ELISA, IP, FuncS | IgG |
| MHH-64 | Recombinant Human Anti-human ANG Antibody | WB, Neut, FuncS | IgG |
| MOB-0614MZ | Mouse Anti-ANG Recombinant Antibody (clone 25028.8) | ELISA, IHC-Fr, IHC-P, WB | Mouse IgG1 |
| MOB-321YJL | Mouse Anti-ANG Recombinant Antibody (clone 26-2F) | ELISA, ICC, IF, IHC-P, IHC-Fr, IP | Mouse IgG1, κ |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-64-F(E) | Recombinant Anti-human ANG Antibody Fab Fragment | RIA, WB, FuncS | Fab |
| MHH-64-F(E) | Recombinant Human Anti-human ANG Antibody Fab Fragment | Neut, WB, FuncS | Fab |
| PFBL-008 | Human Anti-ANG Recombinant Antibody (clone 26-2F); Fab Fragment | WB, IHC, IF, FuncS | Human Fab |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-64-S(P) | Recombinant Anti-human ANG Antibody scFv Fragment | WB, IHC, FuncS | scFv |
| MHH-64-S(P) | Recombinant Human Anti-human ANG Antibody scFv Fragment | FC, Neut, Biosensors, FuncS | scFv |
| PSBL-008 | Human Anti-ANG Recombinant Antibody (clone 26-2F); scFv Fragment | WB, IHC, IF, FuncS | Human scFv |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0325-XY108 | Anti-ANG Immunohistochemistry Kit | IHC | |
| VS-0525-XY362 | Anti-Human ANG Immunohistochemistry Kit | IHC | |
| VS-0525-XY363 | Anti-Mouse ANG Immunohistochemistry Kit | IHC |
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