Hi-Affi™ Rabbit Anti-ANXA2 Recombinant Antibody (clone DS184AB)
CAT#: MOR-0184
Recombinant Rabbit anti-ANXA2 monoclonal antibody for WB, IHC-P, ICC/IF, FC, excellent for producing superior results. Hi-Affi™ antibodies are developed from different sources, such as B cells of immunized animals, hybridoma, phage display library. The application of recombinant technology avoids the traditional ascitic fluid approach, which achieves the antibody generation in a humane way with much larger scale, greater specificity and sensitivity, and not susceptible to cell-line drift or lot-to-lot variation.
Gene Expression
Subcellular Location
Figure 1 IF staining of human cell line U-2 OS
Immunofluorescent staining of human cell line U-2 OS shows localization to plasma membrane & cytosol.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/46964/1865_G3_2_selected.jpg
Normal Tissue
Figure 2 Cerebral cortex
Endothelial cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/61798/138247_B_9_5.jpg
Normal Tissue
Figure 3 Lung
Alveolar cells type I Staining: Medium Intensity: Moderate Quantity:>75% Alveolar cells type I Endothelial cells Staining: Low Intensity: Weak Quantity:>75% Macrophages Staining: High Intensity: Strong
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/61798/138247_A_3_4.jpg
Normal Tissue
Figure 4 Colon
Endothelial cells Staining: Low Intensity: Moderate Quantity: <25% Location: Cytoplasmic/ membranous Glandular cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Peripheral nerve/ganglion Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/61798/138247_A_9_3.jpg
Normal Tissue
Figure 5 Liver
Cholangiocytes Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/61798/138247_A_7_4.jpg
Normal Tissue
Figure 6 Kidney
Cells in glomeruli Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Cells in tubules Staining: Medium Intensity: Strong Quantity: <25% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/61798/138247_A_7_5.jpg
Normal Tissue
Figure 7 Lymph node
Non-germinal center cells Staining: Medium Intensity: Strong Quantity: <25% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/61798/138247_A_8_8.jpg
RNA Expression
Figure 8 RNA cell line category: Low cell line specificity
Cell lines ordered by descending RNA expression order
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000182718-ANXA2
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Specifications
- Immunogen
- Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human Annexin A2 aa 1-100 (Cysteine residue). The exact sequence is proprietary.
- Host Species
- Rabbit
- Derivation
- Phage display library screening
- Type
- Rabbit IgG
- Species Reactivity
- Mouse, Rat, Human
- Clone
- DS184AB
- Applications
- WB, IHC-P, IF, FC
- Conjugate
- Unconjugated
Product Property
- Purification
- Purified with Absolute Mag™ Protein A Agarose
- Purity
- >95% as determined by analysis by SDS-PAGE
- Format
- Liquid
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Target
- Alternative Names
- Annexin A2; Annexin II; Placental Anticoagulant Protein IV; Calpactin I Heavy Chain; Calpactin-1 Heavy Chain; Chromobindin-8; Lipocortin II; Protein I; Annexin-2; ANX2L4; PAP-IV; CAL1H; LPC2D; ANX2; P36; Epididymis Secretory Protein Li 270; Calpactin I Heavy Polypeptide; Chromobindin 8; HEL-S-270; LIP2; LPC2
- Gene ID
- 302
- UniProt ID
- P07355
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Downloadable Resources
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
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| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0325-XY119 | Anti-ANXA2 Immunohistochemistry Kit | IHC | |
| VS-0525-XY411 | Anti-Mouse ANXA2 Immunohistochemistry Kit | IHC | |
| VS-0525-XY410 | Anti-Human ANXA2 Immunohistochemistry Kit | IHC | |
| VS-0525-XY412 | Anti-Chicken ANXA2 Immunohistochemistry Kit | IHC |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS13-YC52 | CytoStream™ Rabbit Anti-ANXA2 Recombinant Antibody (VS13-YC52) | WB, IHC-P, ICC, IF, FC | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0625-FY2 | Mouse Anti-ANXA2 scFv-Fc Chimera (VS-0625-FY2) | FC, IP, ADCC, Cyt | Mouse IgM, scFv-Fc |
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