Hi-Affi™ Rabbit Anti-ARPC5 Recombinant Antibody (clone DS259AB) (CAT#: MOR-0259)
Recombinant Rabbit anti-ARPC5 monoclonal antibody for WB, FC, IHC, ICC, excellent for producing superior results. Hi-Affi™ antibodies are developed from different sources, such as B cells of immunized animals, hybridoma, phage display library. The application of recombinant technology avoids the traditional ascitic fluid approach, which achieves the antibody generation in a humane way with much larger scale, greater specificity and sensitivity, and not susceptible to cell-line drift or lot-to-lot variation.
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Custom Production
We specialize in custom recombinant antibody production, offering seamless execution from provided sequences to high-quality antibody deliverables, ensuring optimal yield and purity.
We require custom production
Subcellular Location and Protein Expression
Normal Tissue
RNA Expression
Figure 1 IF staining of human cell line U-251 MG
(Immunofluorescent staining of human cell line U-251 MG shows localization to cytosol & vesicles.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/31972/789_C2_2_selected.jpg
(Immunofluorescent staining of human cell line U-251 MG shows localization to cytosol & vesicles.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/31972/789_C2_2_selected.jpg
Figure 2 IHC staining of human appendix
(Immunohistochemical staining of human appendix shows cytoplasmic positivity in germinal center.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/22013/49380_A_3_2_selected.jpg
(Immunohistochemical staining of human appendix shows cytoplasmic positivity in germinal center.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/22013/49380_A_3_2_selected.jpg
Figure 3 IF staining of human cell line A-431
(Immunofluorescent staining of human cell line A-431 shows localization to plasma membrane, cell Junctions & vesicles.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/31972/899_C2_2_blue_red_green.jpg
(Immunofluorescent staining of human cell line A-431 shows localization to plasma membrane, cell Junctions & vesicles.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/31972/899_C2_2_blue_red_green.jpg
Figure 4 IF staining of human cell line U-2 OS
(Immunofluorescent staining of human cell line U-2 OS shows localization to cytosol & vesicles.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/31972/776_C2_2_blue_red_green.jpg
(Immunofluorescent staining of human cell line U-2 OS shows localization to cytosol & vesicles.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/31972/776_C2_2_blue_red_green.jpg
Figure 5 Cerebral cortex
(Neuronal cells
Staining:Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous nuclear)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/22013/49380_B_9_5.jpg
(Neuronal cells
Staining:Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous nuclear)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/22013/49380_B_9_5.jpg
Figure 6 Colon
(Glandular cells
Staining:Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous
Peripheral nerve/ganglion
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/22013/49380_A_8_3.jpg
(Glandular cells
Staining:Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous
Peripheral nerve/ganglion
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/22013/49380_A_8_3.jpg
Figure 7 Liver
(Hepatocytes
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/22013/49380_A_9_4.jpg
(Hepatocytes
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/22013/49380_A_9_4.jpg
Figure 8 Kidney
(Cells in tubules
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/22013/49380_A_8_5.jpg
(Cells in tubules
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/22013/49380_A_8_5.jpg
Figure 9 Testis
(Cells in seminiferous ducts
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
Leydig cells
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/22013/49380_A_6_6.jpg
(Cells in seminiferous ducts
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
Leydig cells
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/22013/49380_A_6_6.jpg
Figure 10 RNA cell line category: Low cell line specificity
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000162704-ARPC5
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000162704-ARPC5
Specifications
- Immunogen
- A synthetic peptide corresponding to residues on the N-terminus of human p16-ARC was used as an immunogen.
- Host Species
- Rabbit
- Derivation
- Phage display library screening
- Type
- Rabbit IgG
- Species Reactivity
- Human
- Clone
- DS259AB
- Applications
- WB, FC, IHC, ICC
- Conjugate
- Unconjugated
Product Property
- Purification
- Purified with Absolute Mag™ Protein A Agarose
- Purity
- >95% as determined by analysis by SDS-PAGE
- Format
- Liquid
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Target
- Alternative Names
- Actin Related Protein 2/3 Complex, Subunit 5, 16kDa; Arp2/3 Protein Complex Subunit P16; Arp2/3 Complex 16 KDa Subunit; P16-Arc; ARC16; Actin Related Protein 2/3 Complex, Subunit 5 (16 KD); DJ127C7.3
- Gene ID
- 10092
- UniProt ID
- O15511
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
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IgG Antibody Products
IHC Kit and Antibody Products: Precision Tools for Immunohistochemistry
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MRO-1140-CN | Rabbit Anti-ARPC5 Recombinant Antibody (clone CBACN-414) | WB, IF, IHC, IP, FC | Rabbit IgG |
| VS3-WK794 | Rabbit Anti-ARPC5 Recombinant Antibody (clone SR34-02) | WB, ICC, IHC, FC | Rabbit IgG |
| VS3-FY99 | Recombinant Rabbit Anti-ARPC5 Antibody (clone R08-2B3) | WB, IHC-P, IP | Rabbit IgG |
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