Mouse Anti-Ampicillin Recombinant Antibody (clone aL2) (CAT#: PABW-008)

Figure 1 Phage-ELISA analysis of aL2, aL4, Al5 and aL6 on transferrin-ampicillin-coated plates.

The signals correspond to 1011 phage particles (in 100 ml). Black bars re¯ect speci®c binding. Competitive inhibition was studied by pre-incubation with 1 mM ampicillin (grey bars) and 1 mM 6-APA (white bars).

Burmester, J., Spinelli, S., Pugliese, L., Krebber, A., Honegger, A., Jung, S., ... & PluÈckthun, A. (2001). Selection, characterization and x-ray structure of anti-ampicillin single-chain Fv fragments from phage-displayed murine antibody libraries. Journal of molecular biology, 309(3), 671-685.

Figure 2 Recombinant AL2 and L2 Proteins Inhibit ADK Activity in Vitro.

(A) Autoradiograph of a chromatogram showing AMP generated in ADK reactions preincubated with the indicated proteins before ATP addition, as described in the text.
(B) ADK activity is reduced proportionally by preincubation with increasing amounts of AL2. Reactions contained 10 ng of ADK and varying amounts of His-AL2.
(C) Stoichiometry of inhibition. The graph shows relative ADK activity plotted against increasing His-AL2:ADK ratio. ADK activity (AMP/AMP+ATP) in each reaction was calculated after phosphorimager quantitation of radioactivity in individual spots.

Wang, H., Hao, L., Shung, C. Y., Sunter, G., & Bisaro, D. M. (2003). Adenosine kinase is inactivated by geminivirus AL2 and L2 proteins. The Plant Cell, 15(12), 3020-3032.

Figure 3 AL2 Is in the Nucleus and the Cytoplasm of TGMV-Infected Cells.

Epidermal cells from TGMV-infected plants (top three rows) and mock-inoculated plants (bottom row) were examined under bright-field illumination using differential interference contrast optics. Nuclei were located by DAPI staining (blue; left column), and AL2 protein was detected using AL2 primary antiserum followed by FITC-conjugated secondary antiserum (green; middle column). DAPI + FITC merge images are shown in the right column. The irregular cells shown in the top row were originally positioned over mesophyll, whereas the elongated cells shown in the lower rows were associated with veins.

Wang, H., Hao, L., Shung, C. Y., Sunter, G., & Bisaro, D. M. (2003). Adenosine kinase is inactivated by geminivirus AL2 and L2 proteins. The Plant Cell, 15(12), 3020-3032.

Figure 4 ADK Levels Are Reduced in Transgenic Plants Expressing AL2 and L2 and Also during Geminivirus Infection.

(A) ADK activity is reduced in transgenic N. benthamiana plants expressing AL21-100 and L2. Crude extracts obtained from comparable stem samples from just below the shoot apex were analyzed for ADK activity as described in the text using equal amounts of protein extract from each sample (150 to 400 ng in different experiments). Labeled AMP produced was quantitated using a phosphorimager. The observed differences between nontransgenic and transgenic plants were statistically significant, as determined by Student's t test (P < 0.05). Graph values represent means ± se.
(B) ADK activity is reduced in TGMV- and BCTV-infected plants. Comparable stem samples from just below the shoot apex were obtained from mock-inoculated N. benthamiana plants and plants infected with wild-type BCTV, a BCTV L2 mutant, TGMV, PVX, or CMV and analyzed as described above and in the text. In all cases, the observed differences between virus-infected and control plants proved statistically significant, as determined by Student's t test (P < 0.05). Graph values represent means ± se.

Wang, H., Hao, L., Shung, C. Y., Sunter, G., & Bisaro, D. M. (2003). Adenosine kinase is inactivated by geminivirus AL2 and L2 proteins. The Plant Cell, 15(12), 3020-3032.