Recombinant Mouse Antibody (6A7) Fab Fragment is specific to BAX. 6A7 antibody recognizes an epitope comprised of amino acids 13–19 of Bax, which is only accessible on the activated form.
Figure 1 Detergent-dependent binding of the conformation-sensitive anti-uBax 6A7 monoclonal antibody to Bax.
Anti-uBax 6A7 monoclonal antibody was used to immunoprecipitate Bax from the soluble protein extract of murine thymocytes (lane a) either in the absence of detergent (lane b) or in the presence of Triton X-100 (lane c), Triton X-114 (lane d), polydocanol (lane e), W-1 (lane f), Chaps (lane g), octyl glucoside (lane h), dodecyl maltoside (lane i), Tween 20 (lane j), Brij 35 (lane k), or sodium cholate (lane l). Western blotting analyses of the immunoprecipitated samples were carried out with α mBax 5B7 and α uBcl-XL 2H12 monoclonal antibodies.
Hsu, Y. T., & Youle, R. J. (1998). Bax in murine thymus is a soluble monomeric protein that displays differential detergent-induced conformations. Journal of Biological Chemistry, 273(17), 10777-10783.
Figure 2 Immunofluorescence
Clusterin was colocalized with conformation-altered Bax. HT1080/Clu cells were treated with or without etoposide and co-stained with anti-clusterin (red; anti-Clu) and 6A7 antibodies (green; 6A7).
Zhang, H., Kim, J. K., Edwards, C. A., Xu, Z., Taichman, R., & Wang, C. Y. (2005). Clusterin inhibits apoptosis by interacting with activated Bax. Nature cell biology, 7(9), 909.
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Download resources about recombinant antibody development and antibody engineering to boost your research.
CAT | Product Name | Application | Type |
---|---|---|---|
MOB-101-S(P) | Recombinant Anti-human BAX Antibody scFv Fragment | ELISA, IF, FuncS | scFv |
MHH-101-S(P) | Recombinant Human Anti-human BAX Antibody scFv Fragment | WB, Neut, FuncS | scFv |
PSBC-014 | Mouse Anti-BAX Recombinant Antibody (clone 6A7); scFv Fragment | WB, FuncS | Mouse scFv |
PSBC-255 | Rat Anti-BAX Recombinant Antibody (clone 3C10); scFv Fragment | ELISA, WB, Inhib | Rat scFv |
HPAB-2961LY-S(P) | Human Anti-BAX Recombinant Antibody; scFv Fragment (HPAB-2961LY-S(P)) | ELISA, Inhib | Human scFv |
CAT | Product Name | Application | Type |
---|---|---|---|
IAB-B001(A) | Recombinant Anti-human BaX Intrabody [(D-Arg)9] | ELISA, ICC, FuncS | vhh-(D-Arg)9 |
IAB-B001(G) | Recombinant Anti-human BaX Intrabody [+36GFP] | PCA, Neut, FuncS | vhh-(+36GFP) |
IAB-B001(T) | Recombinant Anti-human BaX Intrabody [Tat] | WB, Neut, FuncS | vhh-Tat |
CAT | Product Name | Application | Type |
---|---|---|---|
TAB-0056CL-VHH | Llama Anti-BAX Recombinant Antibody (TAB-0056CL-VHH) | ELISA, FuncS, Apop, BI | Llama VHH |
TAB-0057CL-VHH | Llama Anti-BAX Recombinant Antibody (TAB-0057CL-VHH) | ELISA, FuncS, Apop, BI | Llama VHH |
TAB-0058CL-VHH | Llama Anti-BAX Recombinant Antibody (TAB-0058CL-VHH) | ELISA, FuncS, Apop, BI | Llama VHH |
TAB-0059CL-VHH | Llama Anti-BAX Recombinant Antibody (TAB-0059CL-VHH) | ELISA, FuncS, Apop, BI | Llama VHH |
TAB-0060CL-VHH | Llama Anti-BAX Recombinant Antibody (TAB-0060CL-VHH) | ELISA, FuncS, Apop, BI | Llama VHH |
CAT | Product Name | Application | Type |
---|---|---|---|
MOB-0900MZ | Mouse Anti-BAX Recombinant Antibody (clone 6A7) | IHC-P | Mouse IgG1 |
PABC-255 | Rat Anti-BAX Recombinant Antibody (clone 3C10) | IP, ELISA, WB, Activ, Inhib | Rat IgG |
MRO-1679-CN | Rabbit Anti-BAX Polyclonal Antibody (MRO-1679-CN) | IF, IHC | Rabbit IgG |
MRO-1680-CN | Rabbit Anti-BAX Polyclonal Antibody (MRO-1680-CN) | WB, IF, IHC | Rabbit IgG |
HPAB-2961LY | Human Anti-BAX Recombinant Antibody (HPAB-2961LY) | ELISA, Inhib | Human IgG |
CAT | Product Name | Application | Type |
---|---|---|---|
NEUT-038CQ | Mouse Anti-BAX Recombinant Antibody (clone 2D2) | WB, IP, FC, IHC, Neut | Mouse IgG1, κ |
CAT | Product Name | Application | Type |
---|---|---|---|
MOR-0328 | Hi-Affi™ Rabbit Anti-BAX Recombinant Antibody (clone DS328AB) | IHC-P | Rabbit IgG |
CAT | Product Name | Application | Type |
---|---|---|---|
PFBC-255 | Rat Anti-BAX Recombinant Antibody (clone 3C10); Fab Fragment | ELISA, WB, Inhib | Rat Fab |
HPAB-2961LY-F(E) | Human Anti-BAX Recombinant Antibody; Fab Fragment (HPAB-2961LY-F(E)) | ELISA, Inhib | Human Fab |
HPAB-2962LY-F(E) | Human Anti-BAX Recombinant Antibody; Fab Fragment (HPAB-2962LY-F(E)) | ELISA, Inhib | Human Fab |
HPAB-2963LY-F(E) | Human Anti-BAX Recombinant Antibody; Fab Fragment (HPAB-2963LY-F(E)) | ELISA, Inhib | Human Fab |
HPAB-2964LY-F(E) | Human Anti-BAX Recombinant Antibody; Fab Fragment (HPAB-2964LY-F(E)) | ELISA, Inhib | Human Fab |
There are currently no Customer reviews or questions for PFBC-014. Click the button above to contact us or submit your feedback about this product.
View the frequently asked questions answered by Creative Biolabs Support.
For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
This site is protected by reCAPTCHA and the Google Privacy Policy and Terms of Service apply.