Mouse Anti-BAX Recombinant Antibody (clone 6A7); Fab Fragment (CAT#: PFBC-014)

Figure 1 Detergent-dependent binding of the conformation-sensitive anti-uBax 6A7 monoclonal antibody to Bax.

Anti-uBax 6A7 monoclonal antibody was used to immunoprecipitate Bax from the soluble protein extract of murine thymocytes (lane a) either in the absence of detergent (lane b) or in the presence of Triton X-100 (lane c), Triton X-114 (lane d), polydocanol (lane e), W-1 (lane f), Chaps (lane g), octyl glucoside (lane h), dodecyl maltoside (lane i), Tween 20 (lane j), Brij 35 (lane k), or sodium cholate (lane l). Western blotting analyses of the immunoprecipitated samples were carried out with α mBax 5B7 and α uBcl-X_L 2H12 monoclonal antibodies.

Hsu, Y. T., & Youle, R. J. (1998). Bax in murine thymus is a soluble monomeric protein that displays differential detergent-induced conformations. Journal of Biological Chemistry, 273(17), 10777-10783.

Figure 2 Immunofluorescence

Clusterin was colocalized with conformation-altered Bax. HT1080/Clu cells were treated with or without etoposide and co-stained with anti-clusterin (red; anti-Clu) and 6A7 antibodies (green; 6A7).

Zhang, H., Kim, J. K., Edwards, C. A., Xu, Z., Taichman, R., & Wang, C. Y. (2005). Clusterin inhibits apoptosis by interacting with activated Bax. Nature cell biology, 7(9), 909.