Provided are anti-BMP4 antibodies binding to a conformational epitope located within the BMPRla binding site of BMP4 and pharmaceutical uses thereof.
Figure 1 Anti-BMP4 VHH specificity to the different BMPs.
Phosphorylation of SMAD1/5/8 in EPC2-hERT cells after activation with 100 ng/mL of human BMP4 in the presence of 5 mg/mL VHHs.
Calpe, S., Wagner, K., El Khattabi, M., Rutten, L., Zimberlin, C., Dolk, E., ... & Krishnadath, K. K. (2015). Effective inhibition of bone morphogenetic protein function by highly specific llama-derived antibodies. Molecular cancer therapeutics, 14(11), 2527-2540.
Figure 2 Anti-BMP4 VHH specificity to the different BMPs.
C2C12 cells were activated with human BMPs in the presence or absence of 5 mg/mL of VHHs or human Fc-Noggin.
Calpe, S., Wagner, K., El Khattabi, M., Rutten, L., Zimberlin, C., Dolk, E., ... & Krishnadath, K. K. (2015). Effective inhibition of bone morphogenetic protein function by highly specific llama-derived antibodies. Molecular cancer therapeutics, 14(11), 2527-2540.
Figure 3 IC50 curves of VHH biheads and monoheads.
C2C12 cells were activated with human BMP4 at 5 ng/mL with increasing concentrations of VHHs.
Calpe, S., Wagner, K., El Khattabi, M., Rutten, L., Zimberlin, C., Dolk, E., ... & Krishnadath, K. K. (2015). Effective inhibition of bone morphogenetic protein function by highly specific llama-derived antibodies. Molecular cancer therapeutics, 14(11), 2527-2540.
Figure 4 Kinetic analysis for BMP binding.
Binding of VHHs to the different BMPs was tested in a direct SPR assay.
Calpe, S., Wagner, K., El Khattabi, M., Rutten, L., Zimberlin, C., Dolk, E., ... & Krishnadath, K. K. (2015). Effective inhibition of bone morphogenetic protein function by highly specific llama-derived antibodies. Molecular cancer therapeutics, 14(11), 2527-2540.
Figure 5 Kinetic analysis for BMP binding.
Epitope binning of VHH-coated chip plates. BMP4 (1.0 mg/mL) was injected on the chip followed by the injection of a second VHH (2.0 mg/mL) or the corresponding controls.
Calpe, S., Wagner, K., El Khattabi, M., Rutten, L., Zimberlin, C., Dolk, E., ... & Krishnadath, K. K. (2015). Effective inhibition of bone morphogenetic protein function by highly specific llama-derived antibodies. Molecular cancer therapeutics, 14(11), 2527-2540.
Figure 6 HADDOCK docking of the VHHs to BMP4 binding.
Function of the generated mutant variants in inhibiting ID1 promoter activity in C2C12 cells after stimulation with BMP4 (5 ng/mL) for 16 hours.
Calpe, S., Wagner, K., El Khattabi, M., Rutten, L., Zimberlin, C., Dolk, E., ... & Krishnadath, K. K. (2015). Effective inhibition of bone morphogenetic protein function by highly specific llama-derived antibodies. Molecular cancer therapeutics, 14(11), 2527-2540.
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
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CAT | Product Name | Application | Type |
---|---|---|---|
MOB-2244z | Mouse Anti-BMP4 Recombinant Antibody (clone 18H6) | WB, ELISA, FC, ICC, IHC | Mouse IgG1 |
MOB-1495MZ | Mouse Anti-BMP4 Recombinant Antibody (clone 20G5C5) | ELISA, ICC, WB | Mouse IgG1 |
ZG-0339F | Mouse Anti-BMP4 Recombinant Antibody (ZG-0339F) | WB, ELISA | Mouse IgG |
VS3-FY162 | Recombinant Rabbit Anti-BMP4 Antibody (clone R01-2G4) | WB, ICC, IF, IP | Rabbit IgG |
VS4-WK50 | Rabbit Anti-BMP4 Recombinant Antibody (clone 19B7) | ELISA, WB, IF | Rabbit IgG |
CAT | Product Name | Application | Type |
---|---|---|---|
TAB-1272CL | Mouse Anti-BMP4 Recombinant Antibody (TAB-1272CL) | FuncS | Mouse IgG2b, κ |
TAB-1273CL | Mouse Anti-BMP4 Recombinant Antibody (TAB-1273CL) | FuncS | Mouse IgG1, κ |
TAB-1272CL-S(P) | Mouse Anti-BMP4 Recombinant Antibody; scFv Fragment (TAB-1272CL-S(P)) | FuncS | Mouse scFv |
TAB-1273CL-S(P) | Mouse Anti-BMP4 Recombinant Antibody; scFv Fragment (TAB-1273CL-S(P)) | FuncS | Mouse scFv |
TAB-1272CL-F(E) | Mouse Anti-BMP4 Recombinant Antibody; Fab Fragment (TAB-1272CL-F(E)) | FuncS | Mouse Fab |
CAT | Product Name | Application | Type |
---|---|---|---|
TAB-0069CL-VHH | Anti-Human BMP4 VHH Single Domain Antibody (Al) | ELISA, WB | Single domain antibody (VHH) |
HPAB-0356-YC-VHH | Recombinant Llama Anti-BMP4 Single Domain Antibody (E7) | Inhib, SPR | Llama VHH |
HPAB-0357-YC-VHH | Recombinant Llama Anti-BMP4 Single Domain Antibody (C4C4) | Inhib, SPR | Llama VHH |
CAT | Product Name | Application | Type |
---|---|---|---|
NEUT-051CQ | Mouse Anti-BMP4 Recombinant Antibody (clone 3H2) | ELISA, Neut, WB | Mouse IgG2b, κ |
NEUT-052CQ | Mouse Anti-BMP4 Recombinant Antibody (clone CBL815) | Neut, WB | Mouse IgG2b |
NEUT-053CQ | Mouse Anti-BMP4 Recombinant Antibody (clone CBL816) | WB, Neut, ELISA | Mouse IgG2b |
NEUT-054CQ | Mouse Anti-BMP4 Recombinant Antibody (clone CBL987) | ELISA, WB, IHC, Neut | Mouse IgG2b, κ |
NEUT-055CQ | Rat Anti-BMP4 Recombinant Antibody (clone CBL387) | Neut | Rat IgG1 |
CAT | Product Name | Application | Type |
---|---|---|---|
MOR-0367 | Hi-Affi™ Rabbit Anti-BMP4 Recombinant Antibody (clone DS367AB) | WB, IHC, ICC, IP, FC | Rabbit IgG |
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