Recombinant Mouse Antibody (20.1) is capable of binding to BTN3A1. This agonist antibody, 20.1, binds to the extracellular domain of BTN3A molecules and induces Vγ9Vδ2 activation with similar kinetics and potency as with addition of PiPs.
Figure 1 HLA haplo-identical HeLa-L and HeLa-M cells vary in their ability to present phosphoantigens to Vγ9Vδ2 T cells.
Activation of Vγ9/Vδ2 T cells detected by ELISA for IFN-γ using a concentration gradient of HMB-PP, agonist antibody CD277 20.1 and T cells expanded from two separate donors. Activation was induced by pretreatment of HeLa cells using anti-BTN3A monoclonal antibody 20.1 for 30 min. Graphs show mean and SD from n = 2 and are representative of two independent measurements.
Rhodes, D. A., Chen, H. C., Williamson, J. C., Hill, A., Yuan, J., Smith, S., ... & Eberl, M. (2018). regulation of human γδ T cells by BTn3a1 Protein stability and aTP-Binding cassette Transporters. Frontiers in immunology, 9, 662.
Figure 2 Transcripts for the three BTN3A isoforms are unchanged in HeLa-L and HeLa-M.
Analysis of activation markers MHC-I (w6/32), MHC-II (L243), and BTN3A (CD277 20.1) by flow cytometry in HeLa cells treated with or without IFN-γ. Results representative of triplicate experiments. Red lines indicate IFN-γ treated and black lines untreated samples. Solid lines are HeLa-M and dotted lines HeLa-L.
Rhodes, D. A., Chen, H. C., Williamson, J. C., Hill, A., Yuan, J., Smith, S., ... & Eberl, M. (2018). regulation of human γδ T cells by BTn3a1 Protein stability and aTP-Binding cassette Transporters. Frontiers in immunology, 9, 662.
Figure 3 Western blot of HeLa-L cells transiently transfected with expression vectors for BTN3A1, BTN3A2, and BTN3A3.
Monoclonal anti-BTN3A (CD277 20.1) antibody and polyclonal sera 056 and B6, directed to B30.2 domains of BTN3A1 and BTN3A3, respectively, were used. Anti-GFP and calnexin (CNX) antibodies provided loading controls for expression of transgene and endogenous proteins, respectively.
Rhodes, D. A., Chen, H. C., Williamson, J. C., Hill, A., Yuan, J., Smith, S., ... & Eberl, M. (2018). regulation of human γδ T cells by BTn3a1 Protein stability and aTP-Binding cassette Transporters. Frontiers in immunology, 9, 662.
Figure 4 CD107a expression on the human Vγ9Vδ2 T cell line GUI following incubation with either 20.1 full-lengthantibody (open circles), 20.1 Fab fragments (filled triangles), or 20.1 scFv (filled circles).
The data are presented as thepercentages of CD107a+ γδT cells and are representative of more than three experiments.
Palakodeti, A., Sandstrom, A., Sundaresan, L., Harly, C., Nedellec, S., Olive, D., ... & Adams, E. J. (2013). The Molecular Basis for Modulation of Human V;# 947; 9V [delta] 2 T Cell Responses by CD277/Butyrophilin-3 (BTN3A)-specific Antibodies. J. Biol. Chem., 287(39); 09, 2012).
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Download resources about recombinant antibody development and antibody engineering to boost your research.
CAT | Product Name | Application | Type |
---|---|---|---|
MOB-127 | Recombinant Anti-human BTN3A1 Antibody | WB, Dot, FuncS | IgG |
MHH-127 | Recombinant Human Anti-human BTN3A1 Antibody | WB, IHC, FuncS | IgG |
PABZ-207 | Recombinant Mouse Anti-BTN3A1 Antibody (mAb103.2) | FACS | IgG |
VS-0723-WK16 | Human Anti-BTN3A1 Recombinant Antibody (VS-0723-WK16) | FC | Human IgG1 |
VS-0723-WK287 | Rabbit Anti-BTN3A1 Recombinant Antibody (VS-0723-WK287) | ELISA, FC | Rabbit IgG |
CAT | Product Name | Application | Type |
---|---|---|---|
MOB-127-F(E) | Recombinant Anti-human BTN3A1 Antibody Fab Fragment | Neut, WB, FuncS | Fab |
MHH-127-F(E) | Recombinant Human Anti-human BTN3A1 Antibody Fab Fragment | IP, IF, FuncS | Fab |
PFBL-411 | Mouse Anti-BTN3A1 Recombinant Antibody (clone 20.1); Fab Fragment | FC, Block | Mouse Fab |
PFBL-412 | Mouse Anti-BTN3A1 Recombinant Antibody (clone 103.2); Fab Fragment | FC, Block | Mouse Fab |
CAT | Product Name | Application | Type |
---|---|---|---|
MOB-127-S(P) | Recombinant Anti-human BTN3A1 Antibody scFv Fragment | FC, IP, FuncS | scFv |
MHH-127-S(P) | Recombinant Human Anti-human BTN3A1 Antibody scFv Fragment | IF, Neut, FuncS | scFv |
PSBL-411 | Mouse Anti-BTN3A1 Recombinant Antibody (clone 20.1); scFv Fragment | FC, Block | Mouse scFv |
PSBL-412 | Mouse Anti-BTN3A1 Recombinant Antibody (clone 103.2); scFv Fragment | FC, Block | Mouse scFv |
CAT | Product Name | Application | Type |
---|---|---|---|
TAB-1312CL | Mouse Anti-BTN3A1 Recombinant Antibody (TAB-1312CL) | FC, FuncS | Mouse IgG |
TAB-1313CL | Mouse Anti-BTN3A1 Recombinant Antibody (TAB-1313CL) | FC | Mouse IgG |
TAB-1314CL | Mouse Anti-BTN3A1 Recombinant Antibody (TAB-1314CL) | ELISA, FC | Mouse IgG |
TAB-1312CL-S(P) | Mouse Anti-BTN3A1 Recombinant Antibody; scFv Fragment (TAB-1312CL-S(P)) | FC, FuncS | Mouse scFv |
TAB-1313CL-S(P) | Mouse Anti-BTN3A1 Recombinant Antibody; scFv Fragment (TAB-1313CL-S(P)) | FC | Mouse scFv |
There are currently no Customer reviews or questions for PABL-414. Click the button above to contact us or submit your feedback about this product.
View the frequently asked questions answered by Creative Biolabs Support.
For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
This site is protected by reCAPTCHA and the Google Privacy Policy and Terms of Service apply.