Anti-C. botulinum neurotoxin type B Recombinant Antibody (BTBH-N1) (CAT#: MRO-140CT)
Recombinant monoclonal antibody specifically binds to C. botulinum neurotoxin type B, expressed in Chinese Hamster Ovary cells(CHO).
Specific Inquiry
ELISA
Figure 1 Detection limit of BoNT/B using ELISA assay.
Immunoassay for BoNT/B with sequential binding of BoNT/B-specific monoclonal antibody (BTBH-N1) and HRP-conjugated goat antimouse IgG1.
Kwon, S. J., Jeong, E. J., Yoo, Y. C., Cai, C., Yang, G. H., Lee, J. C., ... & Lee, K. B. (2014). High sensitivity detection of active botulinum neurotoxin by glyco-quantitative polymerase chain-reaction. Analytical chemistry, 86(5), 2279-2284.
WB
Figure 2 Cross reactivity of BoNT/B-specific mAb, BTBH-N1 to BoNT/A and E.
The other was transferred to a nitrocellulose membrane, and Western blot analysis conducted to identify the cross reactivity of BTBH-N1 to other serotypes of botulinum toxin. Lanes: M, molecular standards; BoNT/A, partially purified BoNT/A complex; BoNT/B, purified non-toxic components-free BoNT/B; and BoNT/E, partially purified BoNT/E.
Yang, G. H., Kim, K. S., Kim, H. W., Jeong, S. T., Huh, G. H., Kim, J. C., & Jung, H. H. (2004). Isolation and characterization of a neutralizing antibody specific to internalization domain of Clostridium botulinum neurotoxin type B. Toxicon, 44(1), 19-25.
Neut
Figure 3 In vivo BTBH-N1 neutralization assay.
Various amounts of BTBH-N1 mAb were mixed with 20 or 100 unit of BoNT/B and injected i.p. The time to death and number of surviving mice were determined. After 92 h, no change was found in the number of surviving mice.
Yang, G. H., Kim, K. S., Kim, H. W., Jeong, S. T., Huh, G. H., Kim, J. C., & Jung, H. H. (2004). Isolation and characterization of a neutralizing antibody specific to internalization domain of Clostridium botulinum neurotoxin type B. Toxicon, 44(1), 19-25.
ELISA
Figure 4 Affinity determination of BTBH-N1 to BoNT/B.
Various concentrations of BoNT/B (10⁻¹¹ - 10⁻⁷ M), previously incubated with purified BTBH-N1 in solution first antibody, were used as competitors for the bound Ag.
Yang, G. H., Kim, K. S., Kim, H. W., Jeong, S. T., Huh, G. H., Kim, J. C., & Jung, H. H. (2004). Isolation and characterization of a neutralizing antibody specific to internalization domain of Clostridium botulinum neurotoxin type B. Toxicon, 44(1), 19-25.
Specifications
- Host Species
- Mouse
- Specificity
- Internalization domain of Clostridium botulinum neurotoxin type B
- Species Reactivity
- C. botulinum
- Clone
- BTBH-N1
- Applications
- ELISA, WB, Neut
Applications
- Application Notes
- The C. botulinum neurotoxin type B antibody has been reported in applications of Enzyme-linked Immunosorbent Assay, Western Blot, Neutralization.
Target
- Alternative Names
- Botulinum neurotoxin; BoNT; B. neurotoxin; C. botulinum neurotoxin type B
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
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Mouse Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MRO-140CT-S(P) | Anti-C. botulinum neurotoxin type B Recombinant Antibody scFv Fragment (BTBH-N1) | ELISA, Neut, WB | |
| MRO-140CT-F(E) | Anti-C. botulinum neurotoxin type B Recombinant Antibody Fab Fragment (BTBH-N1) | ELISA, Neut, WB |
scFv Fragment Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-1005-FY-F(E) | Human Anti-BONT Recombinant Antibody; Fab Fragment (HPAB-1005-FY-F(E)) | Neut | Humanized Fab |
| HPAB-1006-FY-F(E) | Human Anti-BONT Recombinant Antibody; Fab Fragment (HPAB-1006-FY-F(E)) | Neut | Humanized Fab |
| HPAB-1007-FY-F(E) | Human Anti-BONT Recombinant Antibody; Fab Fragment (HPAB-1007-FY-F(E)) | Neut | Humanized Fab |
| HPAB-1008-FY-F(E) | Human Anti-BONT Recombinant Antibody; Fab Fragment (HPAB-1008-FY-F(E)) | Neut | Humanized Fab |
| HPAB-1009-FY-F(E) | Human Anti-BONT Recombinant Antibody; Fab Fragment (HPAB-1009-FY-F(E)) | Neut | Humanized Fab |
Fab Fragment Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-1005-FY-S(P) | Human Anti-BONT Recombinant Antibody; scFv Fragment (HPAB-1005-FY-S(P)) | Neut | Humanized scFv |
| HPAB-1006-FY-S(P) | Human Anti-BONT Recombinant Antibody; scFv Fragment (HPAB-1006-FY-S(P)) | Neut | Humanized scFv |
| HPAB-1007-FY-S(P) | Human Anti-BONT Recombinant Antibody; scFv Fragment (HPAB-1007-FY-S(P)) | Neut | Humanized scFv |
| HPAB-1008-FY-S(P) | Human Anti-BONT Recombinant Antibody; scFv Fragment (HPAB-1008-FY-S(P)) | Neut | Humanized scFv |
| HPAB-1009-FY-S(P) | Human Anti-BONT Recombinant Antibody; scFv Fragment (HPAB-1009-FY-S(P)) | Neut | Humanized scFv |
Recombinant Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0623-WK83 | Human Anti-BoNT Recombinant Antibody (clone 4E17) | ELSIA, FC, Neut | Human IgG |
| VS-0623-WK38 | Llama Anti-BoNT Serotype A Recombinant Antibody (clone A17) | ELISA, MFI, WB | Llama IgG |
| VS-0623-WK39 | Llama Anti-BoNT Serotype A Recombinant Antibody (clone A18) | ELISA, MFI, WB | Llama IgG |
| VS-0623-WK40 | Llama Anti-BoNT Serotype B Recombinant Antibody (clone B4) | ELISA, MFI, WB | Llama IgG |
| VS-0623-WK41 | Llama Anti-BoNT Serotype B Recombinant Antibody (clone B2) | ELISA, MFI, WB | Llama IgG |
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For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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