Mouse Anti-C5 Recombinant Antibody (clone m5G1.1) (CAT#: HPAB-0614-CN)

Figure 1 Inhibition of 5G1.1 mAb binding to human C5 by recombinant humanized h5G1.1 HuG4 mAb.

Biotinylated 5G1. 1 mAb (0.2 nM), plus the indicated concentrations of unlabeled murine 5G1.1 mAb, recombinant h5G1.1 HuG4 mAb or a negative control recombinant HuG4 mAb (ch3F4 HuG4 mAb), were incubated together for 2 hr at 37°C in microtiter plates coated with human C5. After washing, bound biotinylated 5G1. 1 mAb was detected using peroxidase-conjugated streptavidin, with quantitation performed by monitoring hydrolysis of o-phenylenediamine dihydrochloride at 490 nm. Absorbance values obtained in the presence of recombinant inhibitor were normalized in each individual experiment, to the value obtained in the absence of competitor. Results shown are mean values of four determinations.

Thomas, T. C., Rollins, S. A., Rother, R. P., Giannoni, M. A., Hartman, S. L., Elliott, E. A., ... & Evans, M. J. (1996). Inhibition of complement activity by humanized anti-C5 antibody and single-chain Fv. Molecular immunology, 33(17-18), 1389-1401.

Figure 2 Protection of porcine aortic endothelial cells from human serum complement by h5G1.1 HuG4 mAb.

Calceinlabeled PAEC were incubated with HBSS containing 1% BSA and 0.85 mg/ml purified rabbit anti-PAEC polyclonal antibody for 30min at 37°C. The cells were washed and incubated with HBSS containing 1% BSA plus 20% human serum and the indicated concentrations of inhibitor for 30 min at 37°C. Calcein dye release and retention were detemined by measurement of the fluorescence of supernatants and SDS-solubilized cells, respectively, with dye release in the absence of human serum subtracted from all values. Results shown are the mean of three independent determinations. The arrow denotes the predicted concentration of hC5 in 20% serum.

Thomas, T. C., Rollins, S. A., Rother, R. P., Giannoni, M. A., Hartman, S. L., Elliott, E. A., ... & Evans, M. J. (1996). Inhibition of complement activity by humanized anti-C5 antibody and single-chain Fv. Molecular immunology, 33(17-18), 1389-1401.