Anti-Human CA9 Recombinant Antibody (MSC3) (CAT#: TAB-1458CL)
This antibody, which can bind to Carbonic Anhydrase IX (CAIX), is useful in the diagnosis and treatment of cancer and in conditions associated with hypoxia and/or elevated CAIX activity. It has cross-reactivity with Human, Mouse.
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FC
Figure 1 Binding specificity of the purified constructs.
Flow cytometric analysis of Fab MSC 3 on human (A) or murine (B) cell lines induced for 48 h in 0.2% 02 (grey) or normoxia (white).
Figure 2 Carbonic anhydrase activity, normalized to a scale where activity measured in HCTl 16-CAIX and HCTl 16-EV membrane fragments are one and zero, respectively.
Asterisks denote significant reduction in activity (MSC5, MSC 10„MSC12 and MSC8 Fab antibodies).
Specifications
- Host Species
- Human
- Type
- Human IgG1
- Specificity
- Human CA9
- Species Reactivity
- Human, Mouse
- Clone
- MSC3
- Applications
- FC
- Conjugate
- Unconjugated
Product Property
- Purification
- Protein A/G Purified
- Purity
- >95%
- Concentration
- Batch Specific
- Buffer
- PBS
- Storage
- Store at 4°C for short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Applications
- Application Notes
- This antibody has been tested for use in Flow Cytometry.
Target
- Alternative Names
- MN; CAIX; carbonic anhydrase 9; CA-IX; P54/58N; RCC-associated antigen G250; RCC-associated protein G250; carbonate dehydratase IX; carbonic anhydrase IX; carbonic dehydratase; membrane antigen MN; pMW1; renal cell carcinoma-associated antigen G250
- Gene ID
- 768
- UniProt ID
- Q16790
Related Resources
Boreel, Daan F., et al. "Quantitative Imaging of Hypoxic CAIX-Positive Tumor Areas with Low Immune Cell Infiltration in Syngeneic Mouse Tumor Models." Molecular Pharmaceutics 20.4 (2023): 2245-2255. https://doi.org/10.1021/acs.molpharmaceut.3c00045
This study investigates the quantitative imaging of hypoxic CAIX-positive tumor areas with low immune cell infiltration in syngeneic mouse tumor models. The research focuses on developing a radiolabeled antibody targeting murine CAIX to visualize chronic hypoxia in these models and study the immune cell population in these hypoxic areas. Using the anti-mCAIX antibody (MSC3) conjugated to diethylenetriaminepentaacetic acid (DTPA) and radiolabeled with indium-111 ([111In]In-MSC3), the study demonstrated that this tracer specifically binds to CAIX-expressing cells in vitro and accumulates in CAIX-positive areas in vivo. The study highlighted that these CAIX-positive areas are characterized by reduced infiltration of immune cells, underscoring the immunosuppressive nature of chronic hypoxia in tumors. The findings suggest that this imaging technique can be used to optimize immuno- and radiotherapy efficacy by visualizing CAIX expression before or during hypoxia-targeted treatments.
Creative Biolabs provided the crucial human IgG1 anti-mouse CAIX antibody (Cat# TAB-1458CL) for this research. This antibody was pivotal in enabling the conjugation and radiolabeling processes necessary for developing the [111In]In-MSC3 tracer. By facilitating specific binding to CAIX-expressing cells and accumulation in hypoxic tumor regions, the MSC3 antibody allowed for the effective visualization and analysis of CAIX-positive hypoxic areas. This contribution was instrumental in advancing the study's objective of using CAIX as a biomarker for chronic hypoxia and its associated immunosuppressive microenvironment in preclinical models.
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
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Extracellular Vesicle (EV) Antibody Products
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-707 | Anti-CAIX/CA9 Recombinant Antibody (Girentuximab) | WB, FuncS, IF, Neut, ELISA, FC, IP | IgG1 - kappa |
| TAB-H43 | Anti-Human CA9 Recombinant Antibody (TAB-H43) | ELISA, IP, IF, Inhib | IgG1 - kappa |
Customer Reviews and Q&As
Customer Review
Q&As
Michael Lee
Excellent for CA9 Studies
Excellent for CA9 Studies
The Anti-CA9 Antibody (MSC3) has been excellent for our CA9 detection assays. Its specificity and sensitivity are top-notch, providing clear and reliable results. This antibody has significantly improved the accuracy of our experiments and saved us a lot of troubleshooting time.
22.Aug,23
Laura Young
Effective and Dependable
Effective and Dependable
We incorporated this antibody into our immunoassays, and it has been a great asset. The specificity for CA9 is precise, allowing for accurate detection and analysis. The reliable performance has made it an invaluable tool in our lab.
19.Dec,22
David Clark
Consistent High-Quality Results
Consistent High-Quality Results
This antibody has proven to be consistent and dependable in our assays. The high-quality, reproducible results have significantly improved our experiments, making it a crucial part of our research toolkit. The strong affinity and specificity for CA9 have been particularly beneficial.
07.Oct,21
Cite This Product
To accurately reference this product in your publication, please use the following citation information:
(Creative Biolabs Cat# TAB-1458CL, RRID: AB_3111834)
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For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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