Mouse Anti-CAST Recombinant Antibody (clone OTI1E7) (CAT#: MOB-1964MZ)
This antibody is a recombinant mouse monoclonal antibody which specifically reacts with human CAST.
Specific Inquiry
Subcellular Location
Normal Tissue
RNA Expression
Figure 1 IF staining of human cell line A549
(Immunofluorescent staining of human cell line A549 shows localization to plasma membrane, cytosol & endoplasmic reticulum.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/36882/857_F12_1_selected.jpg
(Immunofluorescent staining of human cell line A549 shows localization to plasma membrane, cytosol & endoplasmic reticulum.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/36882/857_F12_1_selected.jpg
Figure 2 IF staining of human cell line HaCaT
(Immunofluorescent staining of human cell line HaCaT shows localization to cytosol & endoplasmic reticulum.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/75906/1908_B9_2_selected.jpg
(Immunofluorescent staining of human cell line HaCaT shows localization to cytosol & endoplasmic reticulum.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/75906/1908_B9_2_selected.jpg
Figure 3 Esophagus
(High expression in esophagus)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/36881/136703_B_5_1_val_selected.jpg
(High expression in esophagus)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/36881/136703_B_5_1_val_selected.jpg
Figure 4 Kidney
(Cells in glomeruli Staining: Low Intensity: Moderate Quantity: <25% Location: Cytoplasmic/ membranous Cells in tubules Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/36881/136703_A_8_5.jpg
(Cells in glomeruli Staining: Low Intensity: Moderate Quantity: <25% Location: Cytoplasmic/ membranous Cells in tubules Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/36881/136703_A_8_5.jpg
Figure 5 Testis
(Cells in seminiferous ducts Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Leydig cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/36881/136703_A_4_6.jpg
(Cells in seminiferous ducts Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Leydig cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/36881/136703_A_4_6.jpg
Figure 6 Skeletal muscle
(Myocytes Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/36881/136703_B_2_6.jpg
(Myocytes Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/36881/136703_B_2_6.jpg
Figure 7 Lymph node
(Germinal center cells Staining: Low Intensity: Moderate Quantity: <25% Location: Cytoplasmic/ membranous Non-germinal center cells Staining: Medium Intensity: Strong Quantity: <25% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/36881/136703_A_8_8.jpg
(Germinal center cells Staining: Low Intensity: Moderate Quantity: <25% Location: Cytoplasmic/ membranous Non-germinal center cells Staining: Medium Intensity: Strong Quantity: <25% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/36881/136703_A_8_8.jpg
Figure 8 RNA cell line category: Low cell line specificity
(Cell lines ordered by descending RNA expression order)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000153113-CAST
(Cell lines ordered by descending RNA expression order)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000153113-CAST
Specifications
- Immunogen
- Recombinant fragment: QPDPDENKPM EDKVKEKAKA EHRDKLGERD DTIPPEYRHL LDDNGQDKPV, corresponding to amino acids 582-670of Human Calpastatin with proprietary tag
- Host Species
- Mouse
- Type
- Mouse IgG1
- Specificity
- Human CAST
- Species Reactivity
- Human
- Clone
- OTI1E7
- Applications
- ELISA, WB
Product Property
- Purity
- >95% as determined by analysis by SDS-PAGE
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Target
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
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See other products for "CAST"
Recombinant Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-2319z | Mouse Anti-CAST Recombinant Antibody (clone 4B3) | WB, FC, IHC | Mouse IgG1 |
Rabbit Monoclonal Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-0483 | Hi-Affi™ Rabbit Anti-CAST Recombinant Antibody (clone DS483AB) | IHC-P | Rabbit IgG |
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For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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