Hi-Affi™ Rabbit Anti-CBX5 Recombinant Antibody (clone DS498AB)
CAT#: MOR-0498
Recombinant Rabbit anti-CBX5 monoclonal antibody for WB, IP, IHC-P, FC, ICC/IF, excellent for producing superior results. Hi-Affi™ antibodies are developed from different sources, such as B cells of immunized animals, hybridoma, phage display library. The application of recombinant technology avoids the traditional ascitic fluid approach, which achieves the antibody generation in a humane way with much larger scale, greater specificity and sensitivity, and not susceptible to cell-line drift or lot-to-lot variation.
Gene Expression
Subcellular Location and Protein Expression
Figure 1 IF staining of human cell line U-2 OS
Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/16699/if_selected.jpg
Subcellular Location and Protein Expression
Figure 2 IF staining of human cell line U-251 MG
Immunofluorescent staining of human cell line U-251 MG shows localization to nucleoplasm.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/16699/164_F10_1_red_green.jpg
Subcellular Location and Protein Expression
Figure 3 IF staining of human cell line A-431
Immunofluorescent staining of human cell line A-431 shows localization to nucleoplasm.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/17548/971_A3_1_red_green.jpg
Normal Tissue
Figure 4 Cerebral cortex
Neuronal cells
Staining:High
Intensity: Strong
Quantity:>75%
Location: Nuclear
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/17548/39008_B_7_5.jpg
Normal Tissue
Figure 5 Colon
Glandular cells
Staining:Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Nuclear
Peripheral nerve/ganglion
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Nuclear
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/17548/39008_A_9_3.jpg
Normal Tissue
Figure 6 Kidney
Cells in glomeruli
Staining:Medium
Intensity: Strong
Quantity: <25%
Location: Nuclear
Cells in tubules
Staining:Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Nuclear
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/17548/39008_A_8_5.jpg
RNA Expression
Figure 7 RNA cell line category: Low cell line specificity
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000094916-CBX5
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Specifications
- Immunogen
- Synthetic peptide corresponding to residues in Human HP1 alpha.
- Host Species
- Rabbit
- Derivation
- Phage display library screening
- Type
- Rabbit IgG
- Species Reactivity
- Human
- Clone
- DS498AB
- Applications
- WB, IP, IHC-P, FC, IF
- Conjugate
- Unconjugated
Product Property
- Purification
- Purified with Absolute Mag™ Protein A Agarose
- Purity
- >95% as determined by analysis by SDS-PAGE
- Format
- Liquid
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Target
- Alternative Names
- Chromobox Homolog 5; Chromobox Homolog 5 (HP1 Alpha Homolog, Drosophila); Heterochromatin Protein 1 Homolog Alpha; Antigen P25; HP1A; Chromobox Homolog 5 (Drosophila HP1 Alpha); Heterochromatin Protein 1-Alpha; HP1 Alpha Homolog (Drosophila); Epididymis Luminal Protein 25; HP1 Alpha Homolog; HP1Hs Alpha; HP1-ALPHA; HP1 Alpha; HEL25; HP1
- Gene ID
- 23468
- UniProt ID
- P45973
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Downloadable Resources
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
See other products for "CBX5"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| DrMAB-276 | Human Anti-CBX5 Recombinant Antibody (clone D244); Fab Fragment | ELISA, IP-MS, ChIP | Human Fab |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| DrMAB-277 | Human Anti-CBX5 Recombinant Antibody (clone D399) | ELISA, IP-MS | Human IgG1 |
| MRO-0764-CN | Mouse Anti-CBX5 Recombinant Antibody (clone B0-1) | WB, IF | Mouse IgG1 |
| MRO-0765-CN | Rabbit Anti-CBX5 Recombinant Antibody (clone CBACN-283) | WB, IF, IHC, IP, FC | Rabbit IgG |
| MOB-0036F | Mouse Anti-CBX5 Recombinant Antibody (clone 2G2) | WB, IHC-P, IF | Mouse IgG |
| MOB-0037F | Mouse Anti-CBX5 Recombinant Antibody (clone 5E3) | IF, WB, IHC-P | Mouse IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0424-XY43 | AbPlus™ Anti-CBX5 Magnetic Beads (D399) | IP-MS, Protein Purification |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0325-XY326 | Anti-CBX5 Immunohistochemistry Kit | IHC | |
| VS-0525-XY1041 | Anti-Mouse CBX5 Immunohistochemistry Kit | IHC | |
| VS-0525-XY1040 | Anti-Human CBX5 Immunohistochemistry Kit | IHC | |
| VS-0525-XY1042 | Anti-Rat CBX5 Immunohistochemistry Kit | IHC |
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