Mouse Anti-CCL2 Recombinant Antibody (TAB-0757CL) (CAT#: TAB-0757CL)
This product is a recombinant mouse antibody that recognizes MCP-1 (monocyte chemoattractant protein 1). The antibody was expressed in mammalian cells with chemically defined culture media and was purified by affinity chromatography.
Specific Inquiry
WB
Figure 1 Anti-CCL2 Antibody (TAB-0757CL) in WB
Western blot analysis of TAB-0757CL was performed with Human CCL2 Protein (His Tag).
TAB-0757CL incubation concentration: 2 ng/μL.
The secondary antibody: HRP-Anti-Mouse IgG (H+L)
Lane 1: Reducing Antigen (0.1μg)
Lane 2: Reducing Antigen (0.3μg)
Lane 3: Reducing Antigen (0.6μg)
ELISA
Figure 2 Anti-CCL2 Antibody (TAB-0757CL) in ELISA
ELISA analysis of TAB-0757CL was performed by coating with Human CCL2 Protein (His Tag).
The secondary antibody: HRP-goat-Anti-Mouse IgG (H+L)
DB
Figure 3 Anti-CCL2 Antibody (TAB-0757CL) in Dot Blot
Dot Blot analysis of TAB-0757CL was performed by coating with Human CCL2 Protein (His Tag).
TAB-0757CL incubation concentration: 2 ng/μL.
The secondary antibody: HRP-Anti-Mouse IgG (H+L)
SEC-HPLC
Figure 4 Mouse Anti-CCL2 Recombinant Antibody (TAB-0757CL) in HPLC
HPLC analysis of TAB-0757CL-L shows the purity is 100%.
SDS-PAGE
Figure 5 Mouse Anti-CCL2 Recombinant Antibody (TAB-0757CL) in SDS-PAGE
SDS-PAGE analysis of TAB-0757CL-L in reduced (Lane 2) and non-reduced (Lane 1) conditions. Gel stained for 30 minutes with Coomassie Blue. As a result of different β-mercaptoethanol-reduced proteins (Heavy chain and Light chain) migrate as about 50 kDa and 23 kDa, respectively. And non-reduced protein migrates as 130-180 kDa.
Specifications
- Immunogen
- Human MCP-1 N1pE-9 peptide
- Host Species
- Mouse
- Type
- Mouse IgG2b
- Specificity
- Human MCP-1 (monocyte chemoattractant protein 1)
- Species Reactivity
- Human
- Applications
- ELISA, SPR, DB, IHC
- Related Disease
- Alzheimer's disease
Product Property
- Purity
- >95% as determined by SDS-PAGE
- Concentration
- See the COA
- Buffer
- PBS
- Preservative
- No preservatives
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Target
- Alternative Names
- CCL2; chemokine (C-C motif) ligand 2; HC11; MCAF; MCP1; MCP-1; SCYA2; GDCF-2; SMC-CF; HSMCR30; C-C motif chemokine 2; small-inducible cytokine A2; monocyte secretory protein JE; monocyte chemotactic protein 1; monocyte chemoattractant protein 1; monocyte chemoattractant protein-1; monocyte chemotactic and activating factor; small inducible cytokine subfamily A (Cys-Cys), member 2; small inducible cytokine A2 (monocyte chemotactic protein 1, homologous to mouse Sig-je)
- Gene ID
- 6347
- UniProt ID
- P13500
Related Resources
Rheumatoid Arthritis
TNF Signaling Pathway
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
Download resources about recombinant antibody development and antibody engineering to boost your research.
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| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-1273z | Mouse Anti-CCL2 Recombinant Antibody (clone 15A10) | ELISA, WB | Mouse IgG1 |
| ZG-0153U | Mouse Anti-CCL2 Recombinant Antibody (clone 3D2E5) | ELISA, IHC | Mouse IgG2b |
| VS3-WK25 | Mouse Anti-CCL2 Recombinant Antibody (clone E11-D8) | WB, ELISA | Mouse IgG1 |
| VS3-XY177 | Mouse Anti-CCL2 Recombinant Antibody (clone 1A7B8) | ELISA, WB | Mouse IgG1 |
| VS3-WK1674 | Recombinant Mouse Anti-CCL2 Capture Antibody (clone 4A2) | ELISA | Mouse IgG2b |
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For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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