Hi-Affi™ Rabbit Anti-CCNB1 Recombinant Antibody (clone DS514AB) (CAT#: MOR-0514)
Recombinant Rabbit anti-CCNB1 monoclonal antibody for FC, ICC, IHC-P, IP, WB, excellent for producing superior results. Hi-Affi™ antibodies are developed from different sources, such as B cells of immunized animals, hybridoma, phage display library. The application of recombinant technology avoids the traditional ascitic fluid approach, which achieves the antibody generation in a humane way with much larger scale, greater specificity and sensitivity, and not susceptible to cell-line drift or lot-to-lot variation.
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Custom Production
We specialize in custom recombinant antibody production, offering seamless execution from provided sequences to high-quality antibody deliverables, ensuring optimal yield and purity.
We require custom production
Subcellular Location
Normal Tissue
RNA Expression
Figure 1 IF staining of human cell line U-251 MG
(Immunofluorescent staining of human cell line U-251 MG shows localization to cytosol.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/30741/if_selected.jpg
(Immunofluorescent staining of human cell line U-251 MG shows localization to cytosol.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/30741/if_selected.jpg
Figure 2 IF staining of human cell line U-2 OS
(Immunofluorescent staining of human cell line U-2 OS shows localization to cytosol.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/61448/1671_B6_5_selected.jpg
(Immunofluorescent staining of human cell line U-2 OS shows localization to cytosol.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/61448/1671_B6_5_selected.jpg
Figure 3 Colon
(Endocrine cells Staining: High Intensity: Strong Enterocytes Staining: Medium Intensity: Strong Quantity: <25% Enterocytes -)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/3804/10580_A_9_3.jpg
(Endocrine cells Staining: High Intensity: Strong Enterocytes Staining: Medium Intensity: Strong Quantity: <25% Enterocytes -)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/3804/10580_A_9_3.jpg
Figure 4 Testis
(Pachytene spermatocytes Staining: High Intensity: Strong Quantity: 75%-25% Preleptotene spermatocytes Staining: Medium Intensity: Strong Quantity: <25% Spermatogonia cells Staining: Medium Intensity: Strong)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/3804/10580_A_4_6.jpg
(Pachytene spermatocytes Staining: High Intensity: Strong Quantity: 75%-25% Preleptotene spermatocytes Staining: Medium Intensity: Strong Quantity: <25% Spermatogonia cells Staining: Medium Intensity: Strong)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/3804/10580_A_4_6.jpg
Figure 5 Lymph node
(Germinal center cells Staining: High Intensity: Strong Quantity: 75%-25% Location: Cytoplasmic/ membranous Non-germinal center cells Staining: Medium Intensity: Strong Quantity: <25% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/3804/10580_A_8_8.jpg
(Germinal center cells Staining: High Intensity: Strong Quantity: 75%-25% Location: Cytoplasmic/ membranous Non-germinal center cells Staining: Medium Intensity: Strong Quantity: <25% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/3804/10580_A_8_8.jpg
Figure 6 RNA cell line category: Low cell line specificity
(Cell lines ordered by descending RNA expression order)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000134057-CCNB1
(Cell lines ordered by descending RNA expression order)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000134057-CCNB1
Specifications
- Immunogen
- Synthetic peptide corresponding to residues in the C-terminus of Human cyclin B1.
- Host Species
- Rabbit
- Derivation
- Phage display library screening
- Type
- Rabbit IgG
- Species Reactivity
- Human
- Clone
- DS514AB
- Applications
- FC, ICC, IHC-P, IP, WB
- Conjugate
- Unconjugated
Product Property
- Purification
- Purified with Absolute Mag™ Protein A Agarose
- Purity
- >95% as determined by analysis by SDS-PAGE
- Format
- Liquid
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Target
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
Download resources about recombinant antibody development and antibody engineering to boost your research.
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IHC Kit and Antibody Products: Precision Tools for Immunohistochemistry
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-1647z | Mouse Anti-CCNB1 Recombinant Antibody (clone 28C1) | WB, IF, IHC, IP, FuncS | Mouse IgG1, κ |
| VS3-FY213 | Recombinant Rabbit Anti-Ccnb1 Antibody (clone R08-9B7) | WB, ICC, IF | Rabbit IgG |
| VS3-FY214 | Recombinant Rabbit Anti-CCNB1 Antibody (clone R01-7H7) | WB, ICC, IF, IP | Rabbit IgG |
| VS3-FY215 | Recombinant Rabbit Anti-CCNB1 Antibody (clone R05-3B3) | WB, IP | Rabbit IgG |
| VS7-HM313 | Mouse Anti-CCNB1 Recombinant Antibody (clone CBL103HM) | WB, IF, ICC, FCM, ELISA | Mouse IgG |
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