Mouse Anti-CCND3 Recombinant Antibody (clone 7I5)
CAT#: MOB-0111CT
Recombinant Mouse monoclonal antibody to Human CCND3, expressed in mammalian cells.
Gene Expression
Subcellular Location
Figure 1 IF staining of human cell line PC-3
Immunofluorescent staining of human cell line PC-3 shows localization to nucleoplasm.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/42871/1432_F9_3_selected.jpg
Normal Tissue
Figure 2 Cerebral cortex
Neuronal cells Staining: Low Intensity: Moderate Quantity: <25% Location: Nuclear Neuropil Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/116/2169_B_9_5.jpg
Normal Tissue
Figure 3 Cerebellum
Cells in granular layer Staining: Low Intensity: Moderate Quantity: <25% Location: Nuclear Cells in molecular layer Staining: High Intensity: Strong Quantity: 75%-25% Location: Nuclear Purkinje cells Staining: High Intensity: Strong Quantity:>75% Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/116/2169_B_8_8.jpg
Normal Tissue
Figure 4 Colon
Glandular cells Staining: Medium Intensity: Strong Quantity: <25% Location: nuclear Peripheral nerve/ganglion Staining: High Intensity: Strong Quantity:>75% Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/116/2169_A_9_3.jpg
Normal Tissue
Figure 5 Testis
Leydig cells Staining: High Intensity: Strong Quantity: 75%-25% Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/116/2169_A_5_6.jpg
Normal Tissue
Figure 6 Lymph node
Germinal center cells Staining: Low Intensity: Moderate Quantity: <25% Location: nuclear Non-germinal center cells Staining: Low Intensity: Moderate Quantity: <25% Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/116/2169_A_7_8.jpg
RNA Expression
Figure 7 RNA cell line category: Cell line enhanced (MOLT-4, REH)
Cell lines ordered by descending RNA expression order
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000112576-CCND3
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Specifications
- Immunogen
- The immunogen consisted of a purified recombinant fragment of the human Cyclin D3 protein, expressed in E. coli and purified using appropriate techniques.
- Host Species
- Mouse
- Type
- Mouse IgG1
- Species Reactivity
- Human
- Clone
- 7I5
- Applications
- ELISA, WB
- Conjugate
- Unconjugated
Product Property
- Purity
- >95% as determined by analysis by SDS-PAGE
- Format
- Liquid
- Buffer
- Preservative:0.03% Sodium azideConstituent:99% Ascites
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Target
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Clear Western Blot Bands
Clone 7I5 delivered clear and specific bands for Cyclin D3 in our Western Blot experiments. This recombinant mouse monoclonal antibody recognized the human CCND3 protein effectively. The ascites-free production method is a significant plus, ensuring we are using a cleaner, more ethical reagent in our cell cycle research.
Reliable ELISA Performance
We incorporated this antibody into our ELISA workflow to measure CCND3 levels in cell lysates. It showed high sensitivity and reproducibility. The high purity (>95%) and specific binding to the recombinant fragment of Human Cyclin D3 made assay validation straightforward and efficient for our lab.
Q&As
-
Does this antibody recognize human CCND3?
A: Yes, this Recombinant Mouse monoclonal antibody (clone 7I5) is raised against a purified recombinant fragment of Human Cyclin D3 and specifically reacts with Human CCND3. It is validated for use in ELISA and Western Blot (WB).
-
What is the formulation of this product?
A: The antibody is supplied as a liquid in ascites containing 0.03% Sodium azide. We recommend centrifuging briefly before use and storing aliquots at -20°C for long-term storage to avoid repeated freeze/thaw cycles.
View the frequently asked questions answered by Creative Biolabs Support.
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Related Signaling Pathways
Hedgehog Signaling Pathway
Downloadable Resources
Download resources about recombinant antibody development and antibody engineering to boost your research.
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
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| CAT | Product Name | Application | Type |
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| CAT | Product Name | Application | Type |
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