Mouse Anti-CCR4 Recombinant Antibody (clone 10E4) (CAT#: HPAB-0149-LSX)
Recombinant monoclonal antibody 10E4 is a IgG1 that binds to a mammalian (e.g., human) CC-chemokine receptor 4 (CCR4) or a portion of the receptor and blocks binding of a ligand to the receptor.
Specific Inquiry
FuncS
Figure 1 Graphs showing that monoclonal antibodies 10E4, 2B10 and 1G1 block the migration of L1.2/CCR4 transfectants to MDC and TARC.
L1.2/CCR4 transfectants (at 2×10⁶ cells per ml in RPMI, 0.5% bovine serum albumin, 10 mM Hepes) were pre-treated with various concentrations of purified anti-CCR4 monoclonal antibodies 10E4 (IgG1), 2B10 (IgG2a) and 1G1 (IgG1) for ten minutes on ice, and then aliquots of 200 μl of cells used in chemotaxis assays with Costar 3.0 μM Transwell Filters (Costar, Cambridge, Mass.) and allowed to chemotax to 50 ng/ml of MDC (FIG. A) or 100 ng/ml of TARC (FIG. B). Cells were then counted on a Becton Dickinson FACSCAN. 10E4 proved to be the best blocking anti-CCR4 monoclonal antibody, followed by 2B10 and then 1G1. An IgG1 control monoclonal antibody had no effect on migration of the L1.2/CCR4 transfectants to MDC (315±30) or TARC (382±11).
Specifications
- Immunogen
- CCR4
- Host Species
- Mouse
- Type
- Mouse IgG1
- Specificity
- Human CCR4
- Species Reactivity
- Human
- Clone
- 10E4
- Applications
- IF, FC, FuncS
Product Property
- Purity
- >95% as determined by SDS-PAGE and HPLC analysis
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- PBS
- Preservative
- No preservatives
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- This antibody has been tested for use in Functional Assay.
Target
- Alternative Names
- CCR4; chemokine (C-C motif) receptor 4; CKR4; K5-5; CD194; CMKBR4; ChemR13; CC-CKR-4; HGCN:14099; C-C chemokine receptor type 4; CCR-4; C-C CKR-4; chemokine (C-C) receptor 4
- Gene ID
- 1233
- UniProt ID
- P51679
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
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See other products for "Clone 10E4"
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Humanized Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-187 | Human Anti-CCR4 Recombinant Antibody (TAB-187) | Neut, ELISA, IF, IP, FuncS, FC, ICC | Human IgG1, κ |
| TAB-1530CL | Human Anti-CCR4 Recombinant Antibody (TAB-1530CL) | Depletion, FuncS | Human IgG4 |
| TAB-1530CL-S(P) | Human Anti-CCR4 Recombinant Antibody; scFv Fragment (TAB-1530CL-S(P)) | Depletion, FuncS | Human scFv |
| TAB-1530CL-F(E) | Human Anti-CCR4 Recombinant Antibody; Fab Fragment (TAB-1530CL-F(E)) | Depletion, FuncS | Humanized Fab |
Immunotoxin
| CAT | Product Name | Application | Type |
|---|---|---|---|
| AGTO-G005D | Anti-CCR4 immunotoxin (scFv)-DT | Cytotoxicity assay, Function study |
Fab Fragment Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| PSBZ-142 | Mouse Anti-CCR4 Recombinant Antibody (clone KM3060); scFv Fragment | WB | Mouse scFv |
| HPAB-0141-LSX-F(E) | Mouse Anti-CCR4 Recombinant Antibody; Fab Fragment (HPAB-0141-LSX-F(E)) | ELISA, WB, FC | Mouse Fab |
| HPAB-0143-LSX-F(E) | Human Anti-CCR4 Recombinant Antibody; Fab Fragment (HPAB-0143-LSX-F(E)) | ELISA, WB, FC | Humanized Fab |
| HPAB-0144-LSX-F(E) | Human Anti-CCR4 Recombinant Antibody; Fab Fragment (HPAB-0144-LSX-F(E)) | ELISA, WB, FC | Humanized Fab |
| HPAB-0145-LSX-F(E) | Human Anti-CCR4 Recombinant Antibody; Fab Fragment (HPAB-0145-LSX-F(E)) | ELISA, WB, FC | Humanized Fab |
Human Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-1521CL-F(E) | Anti-Human CCR4 Recombinant Antibody Fab Fragment (208) | FC, Inhib | Human antibody |
| TAB-1522CL-F(E) | Anti-Human CCR4 Recombinant Antibody Fab Fragment (306) | FC, Inhib | Human antibody |
| TAB-1523CL-F(E) | Anti-Human CCR4 Recombinant Antibody Fab Fragment (308) | FC, Inhib | Human antibody |
| TAB-1524CL-F(E) | Anti-Human CCR4 Recombinant Antibody Fab Fragment (406) | FC, Inhib | Human antibody |
| TAB-1525CL-F(E) | Anti-Human CCR4 Recombinant Antibody Fab Fragment (501) | FC, Inhib | Human antibody |
scFv Fragment Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-1777LY-S(P) | Mouse Anti-CCR4 Recombinant Antibody; scFv Fragment (HPAB-1777LY-S(P)) | FC, Inhib, ELISA | Mouse scFv |
| HPAB-1778LY-S(P) | Mouse Anti-CCR4 Recombinant Antibody; scFv Fragment (HPAB-1778LY-S(P)) | FC, Inhib, ELISA | Mouse scFv |
| HPAB-1779LY-S(P) | Mouse Anti-CCR4 Recombinant Antibody; scFv Fragment (HPAB-1779LY-S(P)) | FC, Inhib, ELISA | Mouse scFv |
| MOB-213YJL-S(P) | Mouse Anti-CCR4 Recombinant Antibody (clone chKM2760); scFv Fragment | ELISA, FC, FuncS | Mouse scFv |
| HPAB-1370WJ-S(P) | Human Anti-CCR4 Recombinant Antibody; scFv Fragment (clone KM2760) | ELISA, WB | Human scFv |
ADCC Enhanced Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| AFC-TAB-187 | Afuco™ Anti-CCR4 Recombinant Antibody (AFC-TAB-187), ADCC Enhanced | Neut, ELISA, IF, IP, FuncS, FC | Human IgG1, κ |
Recombinant Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-1004WJ | Human Anti-CCR4 Recombinant Antibody (clone 17G) | FC | Human IgG1, λ |
| HPAB-1005WJ | Human Anti-CCR4 Recombinant Antibody (clone 9E) | FC | Human IgG1, λ |
| HPAB-1006WJ | Human Anti-CCR4 Recombinant Antibody (clone 1O) | FC, ELISA, WB | Human IgG1, λ |
| HPAB-1007WJ | Human Anti-CCR4 Recombinant Antibody (clone 11F) | FC, ELISA, WB | Human IgG1, κ |
| HPAB-1008WJ | Human Anti-CCR4 Recombinant Antibody (clone 9E10J) | FC, Cyt, Inhib, FuncS, ELISA, WB | Human IgG1, λ |
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For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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