Mouse Anti-CCR4 Recombinant Antibody (clone 10E4)
CAT#: HPAB-0149-LSX
Recombinant monoclonal antibody 10E4 is a IgG1 that binds to a mammalian (e.g., human) CC-chemokine receptor 4 (CCR4) or a portion of the receptor and blocks binding of a ligand to the receptor.
Published Data
Gene Expression
FuncS
Figure 1 Graphs showing that monoclonal antibodies 10E4, 2B10 and 1G1 block the migration of L1.2/CCR4 transfectants to MDC and TARC.
L1.2/CCR4 transfectants (at 2×10⁶ cells per ml in RPMI, 0.5% bovine serum albumin, 10 mM Hepes) were pre-treated with various concentrations of purified anti-CCR4 monoclonal antibodies 10E4 (IgG1), 2B10 (IgG2a) and 1G1 (IgG1) for ten minutes on ice, and then aliquots of 200 μl of cells used in chemotaxis assays with Costar 3.0 μM Transwell Filters (Costar, Cambridge, Mass.) and allowed to chemotax to 50 ng/ml of MDC (FIG. A) or 100 ng/ml of TARC (FIG. B). Cells were then counted on a Becton Dickinson FACSCAN. 10E4 proved to be the best blocking anti-CCR4 monoclonal antibody, followed by 2B10 and then 1G1. An IgG1 control monoclonal antibody had no effect on migration of the L1.2/CCR4 transfectants to MDC (315±30) or TARC (382±11).
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Subcellular Location
Figure 1 IF staining of human cell line U-251 MG
Immunofluorescent staining of human cell line U-251 MG shows localization to plasma membrane.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/31613/329_F5_2_selected.jpg
Normal Tissue
Figure 2 IHC staining of human tonsil
Immunohistochemical staining of human tonsil shows strong cytoplasmic positivity in a subset of cells.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/31613/ihc_selected.jpg
Normal Tissue
Figure 3 Colon
Endothelial cells Staining: Low Intensity: Weak Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/31613/68832_A_8_3.jpg
Normal Tissue
Figure 4 Testis
Leydig cells Staining: Low Intensity: Weak Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/31613/68832_A_4_6.jpg
Normal Tissue
Figure 5 Lymph node
Non-germinal center cells Staining: Medium Intensity: Strong Quantity: <25% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/31613/68832_A_9_8.jpg
RNA Expression
Figure 6 RNA cell line category: Cell line enriched (HDLM-2)
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000183813-CCR4
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Specifications
- Immunogen
- CCR4
- Host Species
- Mouse
- Type
- Mouse IgG1
- Specificity
- Human CCR4
- Species Reactivity
- Human
- Clone
- 10E4
- Applications
- IF, FC, FuncS
Product Property
- Purity
- >95% as determined by SDS-PAGE and HPLC analysis
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- PBS
- Preservative
- No preservatives
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- This antibody has been tested for use in Functional Assay.
Target
REVIEWS AND Q&AS
CITATIONS
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Highly Specific
In our flow cytometry tests, the Mouse Anti-CCR4 Recombinant Antibody (clone 10E4) has demonstrated outstanding specificity. It is a trustworthy option for our research because it regularly yields results that are distinct and easy to understand.
Great for T Cell Studies
Our lab focuses on T cell biology, and this antibody has been instrumental in identifying CCR4-expressing T cells. It is highly sensitive and dependable.
Reliable Reagent
As an antibody for CCR4, the Mouse Anti-CCR4 Recombinant Antibody has proven to be a reliable reagent in our ongoing research. We highly recommend it for anyone working in immunology.
Q&As
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What species is the Mouse Anti-CCR4 Recombinant Antibody (clone 10E4) derived from?
A: The mouse from which the antibody is generated is clone 10E4.
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What is CCR4, and why is it significant in research?
A: The immunological response is mediated by the receptor known as CCR4 (C-C chemokine receptor type 4). Its importance stems from its function in the control and operation of T cells.
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What type of antigen does the Mouse Anti-CCR4 Recombinant Antibody (clone 10E4) target?
A: It goes after the protein known as the CCR4 receptor, which is found on the surface of some immune cells.
View the frequently asked questions answered by Creative Biolabs Support.
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Downloadable Resources
Download resources about recombinant antibody development and antibody engineering to boost your research.
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
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Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
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|---|---|---|---|
| MOB-1992z | Mouse Anti-CCR4 Recombinant Antibody (clone 42C4) | WB, FC, ICC, IF | Mouse IgG2a |
| PABZ-142 | Human Anti-CCR4 Recombinant Antibody (clone KM3060) | WB | Chimeric (mouse/human) IgG1, κ |
| HPAB-0141-LSX | Mouse Anti-CCR4 Recombinant Antibody (HPAB-0141-LSX) | ELISA, WB, FC, ADCC, CDC, FuncS | Mouse IgG2b |
| HPAB-0143-LSX | Human Anti-CCR4 Recombinant Antibody (HPAB-0143-LSX) | ELISA, WB, FC, ADCC, CDC, FuncS | Human IgG |
| HPAB-0144-LSX | Human Anti-CCR4 Recombinant Antibody (HPAB-0144-LSX) | ELISA, WB, FC, ADCC, CDC | Human IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
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| TAB-1530CL | Human Anti-CCR4 Recombinant Antibody (TAB-1530CL) | Depletion, FuncS | Human IgG4 |
| TAB-1530CL-S(P) | Human Anti-CCR4 Recombinant Antibody; scFv Fragment (TAB-1530CL-S(P)) | Depletion, FuncS | Human scFv |
| TAB-1530CL-F(E) | Human Anti-CCR4 Recombinant Antibody; Fab Fragment (TAB-1530CL-F(E)) | Depletion, FuncS | Humanized Fab |
| CAT | Product Name | Application | Type |
|---|---|---|---|
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| CAT | Product Name | Application | Type |
|---|---|---|---|
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| TAB-1525CL-S(P) | Anti-Human CCR4 Recombinant Antibody scFv Fragment (501) | FC, Inhib | Human antibody |
| TAB-1526CL-S(P) | Anti-Human CCR4 Recombinant Antibody scFv Fragment (503) | FC, Inhib | Human antibody |
| TAB-1527CL-S(P) | Anti-Human CCR4 Recombinant Antibody scFv Fragment (601) | FC, Inhib | Human antibody |
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| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-0047-FY-S(P) | Human Anti-CCR4 Recombinant Antibody; scFv Fragment (HPAB-0047-FY-S(P)) | FC | Human scFv |
| HPAB-1777LY-S(P) | Mouse Anti-CCR4 Recombinant Antibody; scFv Fragment (HPAB-1777LY-S(P)) | FC, Inhib, ELISA | Mouse scFv |
| HPAB-1778LY-S(P) | Mouse Anti-CCR4 Recombinant Antibody; scFv Fragment (HPAB-1778LY-S(P)) | FC, Inhib, ELISA | Mouse scFv |
| HPAB-1779LY-S(P) | Mouse Anti-CCR4 Recombinant Antibody; scFv Fragment (HPAB-1779LY-S(P)) | FC, Inhib, ELISA | Mouse scFv |
| HPAB-1780LY-S(P) | Human Anti-CCR4 Recombinant Antibody; scFv Fragment (HPAB-1780LY-S(P)) | FC, Inhib, ELISA | Human scFv |
| CAT | Product Name | Application | Type |
|---|---|---|---|
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| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-1004WJ-F(E) | Human Anti-CCR4 Recombinant Antibody (clone 17G); Fab Fragment | ELISA, WB | Human Fab |
| HPAB-1005WJ-F(E) | Human Anti-CCR4 Recombinant Antibody (clone 9E); Fab Fragment | ELISA, WB | Human Fab |
| HPAB-1006WJ-F(E) | Human Anti-CCR4 Recombinant Antibody (clone 1O); Fab Fragment | ELISA, WB | Human Fab |
| HPAB-1007WJ-F(E) | Human Anti-CCR4 Recombinant Antibody (clone 11F); Fab Fragment | ELISA, WB | Human Fab |
| MOB-213YJL-F(E) | Chimeric (Mouse/Human) Anti-CCR4 Recombinant Antibody (clone chKM2760); Fab Fragment | ELISA, FC, FuncS | Chimeric (mouse/human) Fab |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0424-XY47 | AbPlus™ Anti-CCR4 Magnetic Beads (VS-0424-XY47) | IP, Protein Purification |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0924-YC105 | Mouse Anti-CCR4 Recombinant Antibody Hexamer (VS-0924-YC105), CDC Enhanced | ELISA, WB, FC, ADCC, CDC | Antibody hexamer |
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| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0325-FY56 | Human Anti-CCR4 (clone 208) scFv-Fc Chimera | FC, Inhib | Human IgG1, scFv-Fc |
| CAT | Product Name | Application | Type |
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| VS-0425-YC219 | Recombinant Anti-CCR4 Vesicular Antibody, EV Displayed (VS-0425-YC219) | ELISA, FC, Neut, Cell-uptake |
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