Mouse Anti-CD151 Recombinant Antibody (clone 9B)
CAT#: HPAB-0214-CN
A monoclonal antibody that binds to the CD151 protein. This product is capable of inhibiting the development of primary tumors and of inhibiting its metastasispromoting activity.
Published Data
Gene Expression
WB
Figure 1 Characterization of CD151 mAb 9B.
Western blot analysis for CD151 in HCCLM3 cells and recombinant CD151 protein using CD151 mAb 9B.
Ke, A. W., Zhang, P. F., Shen, Y. H., Gao, P. T., Dong, Z. R., Zhang, C., ... & Kang, Q. (2016). Generation and characterization of a tetraspanin CD151/integrin α6β1-binding domain competitively binding monoclonal antibody for inhibition of tumor progression in HCC. Oncotarget, 7(5), 6314.
Co-IP
Figure 2 Characterization of CD151 mAb 9B.
Co-IP assay using anti-human CD151 mAb 9B in HCCLM3 cells.
Ke, A. W., Zhang, P. F., Shen, Y. H., Gao, P. T., Dong, Z. R., Zhang, C., ... & Kang, Q. (2016). Generation and characterization of a tetraspanin CD151/integrin α6β1-binding domain competitively binding monoclonal antibody for inhibition of tumor progression in HCC. Oncotarget, 7(5), 6314.
Inhib
Figure 3 Characterization of CD151 mAb 9B.
CD151 mAb 9B inhibits Akt signaling in HCCLM3 cells.
Ke, A. W., Zhang, P. F., Shen, Y. H., Gao, P. T., Dong, Z. R., Zhang, C., ... & Kang, Q. (2016). Generation and characterization of a tetraspanin CD151/integrin α6β1-binding domain competitively binding monoclonal antibody for inhibition of tumor progression in HCC. Oncotarget, 7(5), 6314.
IF
Figure 4 Localization and Expression of CD151 Protein in HCCs.
Immunofluorescent staining with CD151 mAb 9B in HCCLM3 cells.
Ke, A. W., Zhang, P. F., Shen, Y. H., Gao, P. T., Dong, Z. R., Zhang, C., ... & Kang, Q. (2016). Generation and characterization of a tetraspanin CD151/integrin α6β1-binding domain competitively binding monoclonal antibody for inhibition of tumor progression in HCC. Oncotarget, 7(5), 6314.
IHC
Figure 5 Localization and Expression of CD151 Protein in HCCs.
Immunohistochemical staining with CD151 mAb 9B in HCC tissues.
Ke, A. W., Zhang, P. F., Shen, Y. H., Gao, P. T., Dong, Z. R., Zhang, C., ... & Kang, Q. (2016). Generation and characterization of a tetraspanin CD151/integrin α6β1-binding domain competitively binding monoclonal antibody for inhibition of tumor progression in HCC. Oncotarget, 7(5), 6314.
FC
Figure 6 Localization and Expression of CD151 Protein in HCCs.
FACS analysis of CD151 mAb 9B binding to the QRD194-196 conformational epitope of CD151 in HCC cells.
Ke, A. W., Zhang, P. F., Shen, Y. H., Gao, P. T., Dong, Z. R., Zhang, C., ... & Kang, Q. (2016). Generation and characterization of a tetraspanin CD151/integrin α6β1-binding domain competitively binding monoclonal antibody for inhibition of tumor progression in HCC. Oncotarget, 7(5), 6314.
FuncS
Figure 7 The effect of CD151 mAb 9B on HCCs.
Wound healing assay for the effect of CD151 mAb 9B on the mobility of tumor cells.
Ke, A. W., Zhang, P. F., Shen, Y. H., Gao, P. T., Dong, Z. R., Zhang, C., ... & Kang, Q. (2016). Generation and characterization of a tetraspanin CD151/integrin α6β1-binding domain competitively binding monoclonal antibody for inhibition of tumor progression in HCC. Oncotarget, 7(5), 6314.
Inhib
Figure 8 CD151 mAb 9B Inhibited the progression of HCCs in vivo.
IHC staining for CD34 and CD151 to assay the effect of CD151 mAb 9B on neoangiogenesis.
Ke, A. W., Zhang, P. F., Shen, Y. H., Gao, P. T., Dong, Z. R., Zhang, C., ... & Kang, Q. (2016). Generation and characterization of a tetraspanin CD151/integrin α6β1-binding domain competitively binding monoclonal antibody for inhibition of tumor progression in HCC. Oncotarget, 7(5), 6314.
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Normal Tissue
Figure 1 Cerebral cortex
Endothelial cells
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/2428/6397_B_7_5.jpg
Normal Tissue
Figure 2 Colon
Endothelial cells
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
Glandular cells
Staining:High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/2428/6397_A_7_3.jpg
Normal Tissue
Figure 3 Liver
Cholangiocytes
Staining:High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous
Hepatocytes
Staining:Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/2428/6397_A_7_4.jpg
Normal Tissue
Figure 4 Kidney
Cells in glomeruli
Staining:High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous
Cells in tubules
Staining:Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/2428/6397_A_8_5.jpg
Normal Tissue
Figure 5 Lymph node
Germinal center cells
Staining:Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/2428/6397_A_7_8.jpg
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Specifications
- Immunogen
- CD151-Integrin α6β1-KLH protein complex (GQRDHASNIYKVEGGC-KLH)
- Host Species
- Mouse
- Type
- Mouse IgG1
- Specificity
- Human CD151
- Species Reactivity
- Human
- Clone
- 9B
- Applications
- WB, Co-IP, IF, IHC, Inhib, FuncS, FC, ELISA
- Related Disease
- Tumor
Product Property
- Purity
- >95% as determined by SDS-PAGE and HPLC analysis
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- PBS
- Preservative
- No preservatives
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- The CD151 antibody has been reported in applications of Western Blot, Co-Immunoprecipitation, Immunofluorescence, Immunohistochemistry, Inhibition, Functional Assay, Flow Cytometry, Enzyme-linked Immunosorbent Assay.
For Western Blot: CD151 mAb 9B (1:2000) was used to detect the expression of CD151 in three HCC cell lines and tumor samples from 30 patients. β-actin (1:5,000) was used as an internal control.
For Co-Immunoprecipitation: Five milligrams of CD151 mAb 9B was used to extract CD151 from the HCCLM3 lysates (250 mg). The gel was stained using the PlusOne Coomassie brilliant blue.
For Immunofluorescence: The expression and localization of CD151 protein in HCCLM3 cells were assayed using the CD151 mAb 9B (1:200).
For Immunohistochemistry: CD151 mAb 9B (1:200) detected the expression of CD151 in the HCC samples.
For Flow Cytometry: A total of 1 × 106 cells were collected by centrifugation and incubated with 20 μg/ml of either CD151 mAb 9B or IgG in PBS containing 1% newborn calf serum for 45 min at 4°C. After being washed with cold PBS three times, the cells were incubated for an additional 45 min at 4°C with a FITC-conjugated secondary antibody in the dark. The cells were then analyzed by FACS cytometry.
Target
- Alternative Names
- CD151 Molecule (Raph Blood Group); Platelet-Endothelial Tetraspan Antigen 3; CD151 Antigen (Raph Blood Group); Membrane Glycoprotein SFA-1; Tetraspanin-24; CD151 Antigen 2; Tspan-24; TSPAN24; PETA-3; GP27; Platelet-Endothelial Cell Tetraspan Antigen 3; Platelet Surface Glycoprotein Gp27; Hemidesmosomal Tetraspanin CD151; MER2; RAPH; SFA1
- Gene ID
- 977
- UniProt ID
- P48509
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
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Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
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- CAT
- Product Name
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| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-1623z | Mouse Anti-CD151 Recombinant Antibody (clone 24G4) | WB, FC, ICC, IHC, FuncS | Mouse IgG1 |
| MOB-0383CT | Mouse Anti-CD151 Recombinant Antibody (clone 22H6b) | ELISA, FC, IF, IHC-Fr, IHC-P, IP, WB | Mouse IgG1 |
| HPAB-0212-CN | Mouse Anti-CD151 Recombinant Antibody (HPAB-0212-CN) | FC, IP, IHC, IF, Inhib, WB | Mouse IgG |
| FAMAB-1034CQ | Human Anti-CD151 Recombinant Antibody (clone 11G5a) | IP, FC, FuncS, IHC | Human IgG1 |
| VS3-XY208 | Mouse Anti-CD151 Recombinant Antibody (clone 3G1) | WB, IP, IF, IHC | Mouse IgG1 |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-1601CL | Anti-Human CD151 Recombinant Antibody (203B6) | FC, FuncS | |
| TAB-1602CL | Anti-Human CD151 Recombinant Antibody (205H8) | FC, FuncS | |
| TAB-1603CL | Anti-Human CD151 Recombinant Antibody (211F3) | FC, FuncS | |
| TAB-1604CL | Anti-Human CD151 Recombinant Antibody (214B2) | FC, FuncS | |
| TAB-1601CL-F(E) | Anti-Human CD151 Recombinant Antibody Fab Fragment (203B6) | FC, FuncS |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-1605CL | Anti-Human CD151 Recombinant Antibody (c203B6) | ELISA, FC, WB | Chimeric antibody (mouse/human) |
| TAB-1606CL | Anti-Human CD151 Recombinant Antibody (c214B2) | ELISA, FC, WB, Block, FuncS | Chimeric antibody (mouse/human) |
| TAB-1605CL-S(P) | Anti-Human CD151 Recombinant Antibody scFv Fragment (c203B6) | ELISA, FC, WB | Chimeric antibody (mouse/human) |
| TAB-1606CL-S(P) | Anti-Human CD151 Recombinant Antibody scFv Fragment (c214B2) | ELISA, FC, WB, Block, FuncS | Chimeric antibody (mouse/human) |
| TAB-1605CL-F(E) | Anti-Human CD151 Recombinant Antibody Fab Fragment (c203B6) | ELISA, FC, WB | Chimeric antibody (mouse/human) |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-1607CL | Anti-Human CD151 Recombinant Antibody (Humanized 203B6) | FC, FuncS, WB | Humanized antibody |
| TAB-1608CL | Anti-Human CD151 Recombinant Antibody (Humanized 214B2) | FC, FuncS, WB | Humanized antibody |
| TAB-1607CL-S(P) | Anti-Human CD151 Recombinant Antibody scFv Fragment (Humanized 203B6) | FC, FuncS, WB | Humanized antibody |
| TAB-1608CL-S(P) | Anti-Human CD151 Recombinant Antibody scFv Fragment (Humanized 214B2) | FC, FuncS, WB | Humanized antibody |
| TAB-1607CL-F(E) | Anti-Human CD151 Recombinant Antibody Fab Fragment (Humanized 203B6) | FC, FuncS, WB | Humanized antibody |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| NEUT-285CQ | Mouse Anti-CD151 Recombinant Antibody (clone 50-6) | FC, ICC, WB, Block | Mouse IgG1, κ |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-0533 | Hi-Affi™ Rabbit Anti-CD151 Recombinant Antibody (clone DS533AB) | WB, IHC, FC | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-0212-CN-S(P) | Mouse Anti-CD151 Recombinant Antibody scFv Fragment (HPAB-0212-CN-S(P)) | WB, FC, IHC, IP | Mouse scFv |
| HPAB-0213-CN-S(P) | Mouse Anti-CD151 Recombinant Antibody scFv Fragment (HPAB-0213-CN-S(P)) | WB, FC, IHC, IP | Mouse scFv |
| HPAB-0214-CN-S(P) | Mouse Anti-CD151 Recombinant Antibody (clone 9B); scFv Fragment | WB, ELISA, IHC, IF, Co-IP | Mouse scFv |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-0212-CN-F(E) | Mouse Anti-CD151 Recombinant Antibody Fab Fragment (HPAB-0212-CN-F(E)) | WB, FC, IHC, IP | Mouse Fab |
| HPAB-0213-CN-F(E) | Mouse Anti-CD151 Recombinant Antibody Fab Fragment (HPAB-0213-CN-F(E)) | WB, FC, IHC, IP | Mouse Fab |
| HPAB-0214-CN-F(E) | Mouse Anti-CD151 Recombinant Antibody (clone 9B); Fab Fragment | WB, ELISA, IHC, IF, Co-IP | Mouse Fab |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0225-XY18 | CytoStream™ Mouse Anti-CD151 Recombinant Antibody (VS-0225-XY18) | FC, IHC, IP | Mouse IgG1, kappa |
| VS-0225-XY19 | CytoStream™ Rat Anti-CD151 Recombinant Antibody (VS-0225-XY19) | FC | Rat IgG2a, kappa |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0325-FY33 | Mouse Anti-CD151 scFv-Fc Chimera (VS-0325-FY33) | WB, IP, IHC, IF | Mouse IgG1, scFv-Fc |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0325-XY352 | Anti-CD151 Immunohistochemistry Kit | IHC |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0425-YC232 | Recombinant Anti-CD151 Vesicular Antibody, EV Displayed (VS-0425-YC232) | ELISA, FC, Cell-uptake |
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