Anti-Human CD151 Recombinant Antibody (c214B2) (CAT#: TAB-1606CL)

Figure 1 The specificity study of chimeric antibodies c203B6[IgG1] and c214B2[IgG1] by western blot.

Figure 2 The inhibition of binding of murine Mabs 203B6 and 214B2 to recombinant EC2 by their respective chimeric forms: c214B2[IgG1]

Figure 3 Binding of chimeric antibodies to PC3 cells with c214B2[IgG1]

Figure 4 The in vivo activity of the c214B2[IgG1] Mab on tumor growth of PC3, an androgen-independent prostate cell line.

Figure 5 A Microscope analysis of the effect of different forms of Mab 214B2 on PC3 cell adhesion.

Figure 6 An analysis of the effect of different forms of Mab 214B2 on PC3 cell adhesion using an ATP assay.

In each well the number of adhered cells was determined using PC3 standard curve, from 0 to 200 000 cells/well. The results are presented as follows: the untreated cells are taken as reference (100%) and the treated cells are presented as the % of reference.

Figure 7 The Cross blocking of the murine antibody m24B2 by the chimeric 241B2 and different variants of the humanized 214B2.

The activity of the humanized variants of 214B2 (hz214B2) to cross block the parental antibody m214B2 was evaluated by ELISA using the recombinant extracellular loop 2 (EC2) of human antigen CD 151. The activity of the humanized variants was compared to the chimeric 214B2. All combinations of VH variant 2 and 3 showed a strong activity that was similar to that of the chimeric antibody.

Figure 8 The Flow cytometry analysis of the humanized antibodies hz214B2 by cross blocking the biotinylated antibody m24B2.

Humanized variants of 214B2 that cross blocked the parental antibody in an EC2 ELISA were further evaluated in flow cytometry. The biotinylated antibody m214B2 was used to detect human CD 151 on PC3 cells. This binding was blocked with different concentrations of humanized variants of 214B2. The chimeric 214B2 antibody was used as a reverence. The humanized variants 3 and 4 (Hz214B2VHVar3VLVar3 and Hz214B2VHVar4VLVar4) showed the strongest cross blocking activity (B).

Figure 9 The BIACore analysis of the affinity of the antibodies c214B2 (Gl), c214B2 (G2), Hz214B2VHVar3VLVar3 (Gl) and Hz214B2VHVar4VLVar4 (Gl).

A, BIACore sensorgram of the antibodies Hz214B2VHVar3VLVar3 (Gl), Hz214B2VHVar4VLVar4 (Gl) and c214B2 (Gl). B, Affinity constant and half life the antibody-EC2 complex.

Figure 10 The in vivo activity of various isotypes of the chimeric c214B2 Mab on the PC3 xenograft model wherein A: c214B2[IgG4] and [IgG1](TH7) and B: c214B2[IgG2].