Recombinant monoclonal antibody to CD22. Clone MJ-7 is a human monoclonal antibody that can be potentially used in the treatment of B-cell malignancy.
Figure 1 Western blot analysis of purified MJ-7.
Size of molecular weight markers are indicated (kDa).
Arndt, M. A., Krauss, J., Schwarzenbacher, R., Vu, B. K., Greene, S., & Rybak, S. M. (2003). Generation of a highly stable, internalizing anti‐CD22 single‐chain Fv fragment for targeting non‐Hodgkin's lymphoma. International journal of cancer, 107(5), 822-829.
Figure 2 Immunoreactivity of monomeric MJ-7 (closed triangle) and MLZ-wt (closed square) after incubation in human serum at 37°C over a period of 6 days.
Arndt, M. A., Krauss, J., Schwarzenbacher, R., Vu, B. K., Greene, S., & Rybak, S. M. (2003). Generation of a highly stable, internalizing anti‐CD22 single‐chain Fv fragment for targeting non‐Hodgkin's lymphoma. International journal of cancer, 107(5), 822-829.
Figure 3 Biophysical stability of IMAC-purified scFv variants.
Immunoreactivity with CD22+ Raji cells was assayed by flow cytometry after incubating the constructs at 37°C in human serum for various time periods. The half-life was determined as the time point with 50% of the initial binding activity.
Arndt, M. A., Krauss, J., Schwarzenbacher, R., Vu, B. K., Greene, S., & Rybak, S. M. (2003). Generation of a highly stable, internalizing anti‐CD22 single‐chain Fv fragment for targeting non‐Hodgkin's lymphoma. International journal of cancer, 107(5), 822-829.
Figure 4 Affinity and specificity of wild-type scFv and stability engineered scFv.
(a) Equilibrium-binding curves for MAb LL2 (open circle); MLZ-wt (closed square) and MJ-7 (closed triangle) as determined by flow cytometry. Binding activity to Raji cells at indicated concentrations is shown as median fluorescence intensity (MFI) minus background fluorescence. Measurements were performed in triplicate; standard deviations are shown as bars. Binding affinity constants (Kd) were determined by fitting the cell binding data to the nonlinear regression model according to the Levenberg-Marquard method. (b) Epitope specificity was determined by competition experiments and analyzed by flow cytometry. MLZ-wt (closed square) and MJ-7 (closed triangle) competed with the MAb LL2 for binding to the CD22 antigen on Raji cells. Results are shown as percent binding inhibition of MAb LL2 at a concentration of 100 nM when incubating tumor cells with various concentrations of scFvs.
Arndt, M. A., Krauss, J., Schwarzenbacher, R., Vu, B. K., Greene, S., & Rybak, S. M. (2003). Generation of a highly stable, internalizing anti‐CD22 single‐chain Fv fragment for targeting non‐Hodgkin's lymphoma. International journal of cancer, 107(5), 822-829.
Figure 5 Internalization of antibodies into living tumor cells.
Alexa-488-labeled MAb LL2 (a,b) or scFv MJ-7 (c,d), each at a concentration of 50 μg/ml, were incubated with Raji cells (5 × 10⁵) for 30 min either at 4°C (a,c) or 37°C (b,d) before analysis by confocal microscopy.
Arndt, M. A., Krauss, J., Schwarzenbacher, R., Vu, B. K., Greene, S., & Rybak, S. M. (2003). Generation of a highly stable, internalizing anti‐CD22 single‐chain Fv fragment for targeting non‐Hodgkin's lymphoma. International journal of cancer, 107(5), 822-829.
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
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CAT | Product Name | Application | Type |
---|---|---|---|
TAB-183-F(E) | Anti-Human CD22 Recombinant Antibody (Moxetumomab Pasudotox) | FuncS, IF, Neut, ELISA, FC, IP, ICC | Fv - disulfide stabilized |
TAB-1714CL | Mouse Anti-CD22 Recombinant Antibody (TAB-1714CL) | ELISA, WB | Mouse IgG |
TAB-1728CL | Anti-Human CD22 Recombinant Antibody (RFB4) | IF, FC | |
TAB-003LC | Mouse Anti-Human CD22 Recombinant Antibody | FC | |
TAB-004LC | Anti-Human CD22 Recombinant Antibody | FC |
CAT | Product Name | Application | Type |
---|---|---|---|
AGTO-G077E | Anti-CD22 immunotoxin RFB4 (IgG)-PE | Cytotoxicity assay, Function study | |
AGTO-G077D | Anti-CD22 immunotoxin RFB4 (IgG)-DT | Cytotoxicity assay, Function study | |
AGTO-G077G | Anti-CD22 immunotoxin RFB4 (IgG)-Gel | Cytotoxicity assay, Function study | |
AGTO-G077R | Anti-CD22 immunotoxin RFB4 (IgG)-RTA | Cytotoxicity assay, Function study | |
AGTO-G077S | Anti-CD22 immunotoxin RFB4 (IgG)-Sap | Cytotoxicity assay, Function study |
CAT | Product Name | Application | Type |
---|---|---|---|
TAB-0196CL | Anti-Human CD22 Recombinant Antibody (hLL2) | Inhib, Cyt, FC, WB | |
TAB-1721CL-S(P) | Anti-Human CD22 Recombinant Antibody scFv Fragment (CD22.1) | ELISA, FC, FuncS | Human antibody |
TAB-1722CL-S(P) | Anti-Human CD22 Recombinant Antibody scFv Fragment (CD22.2) | ELISA, FC, FuncS | Human antibody |
TAB-1721CL-F(E) | Anti-Human CD22 Recombinant Antibody Fab Fragment (CD22.1) | ELISA, FC, FuncS | Human antibody |
TAB-1722CL-F(E) | Anti-Human CD22 Recombinant Antibody Fab Fragment (CD22.2) | ELISA, FC, FuncS | Human antibody |
CAT | Product Name | Application | Type |
---|---|---|---|
TAB-1725CL | Human Anti-CD22 Recombinant Antibody (TAB-1725CL) | Block, Cyt, ELISA, FuncS | Humanized IgG |
TAB-1729CL | Human Anti-CD22 Recombinant Antibody (TAB-1729CL) | IF, FC | Humanized IgG |
TAB-002LC | Anti-Human CD22 Recombinant Antibody (VM1000) | FC, ELISA, SPR, FuncS | Humanized antibody |
TAB-002LC-S(P) | Anti-Human CD22 Recombinant Antibody scFv Fragment (VM1000) | ELISA, SPR | Humanized antibody |
TAB-002LC-F(E) | Anti-Human CD22 Recombinant Antibody Fab Fragment (VM1000) | ELISA, SPR | Humanized antibody |
CAT | Product Name | Application | Type |
---|---|---|---|
TAB-1724CL-S(P) | Mouse Anti-CD22 Recombinant Antibody; scFv Fragment (TAB-1724CL-S(P)) | Inhib | Mouse scFv |
TAB-1724CL-F(E) | Human Anti-CD22 Recombinant Antibody; Fab Fragment (TAB-1724CL-F(E)) | Inhib | Chimeric (mouse/human) Fab |
TAB-001LC | Human Anti-CD22 Recombinant Antibody (TAB-001LC) | ELISA | Chimeric antibody (mouse/human) |
TAB-001LC-S(P) | Mouse Anti-CD22 Recombinant Antibody; scFv Fragment (TAB-001LC-S(P)) | ELISA | Mouse scFv |
TAB-001LC-F(E) | Human Anti-CD22 Recombinant Antibody; Fab Fragment (TAB-001LC-F(E)) | ELISA | Chimeric antibody (mouse/human) |
CAT | Product Name | Application | Type |
---|---|---|---|
Gly-001LC | Recombinant Anti-Human CD22 Antibody (Fab glycosylation) | ELISA, FC | Mouse antibody |
CAT | Product Name | Application | Type |
---|---|---|---|
Gly-111LC | Recombinant Anti-Human CD22 Antibody (Fc glycosylation) | ELISA | Chimeric antibody (mouse/human) |
CAT | Product Name | Application | Type |
---|---|---|---|
NEUT-315CQ | Rat Anti-CD22 Recombinant Antibody (clone 2D6) | Block | Rat IgG1, κ |
CAT | Product Name | Application | Type |
---|---|---|---|
MOR-0545 | Hi-Affi™ Rabbit Anti-CD22 Recombinant Antibody (clone DS545AB) | IP, WB | Rabbit IgG |
CAT | Product Name | Application | Type |
---|---|---|---|
HPAB-0194-LSX-F(E) | Mouse Anti-CD22 Recombinant Antibody; Fab Fragment (HPAB-0194-LSX-F(E)) | ELISA | Mouse Fab |
HPAB-0195-LSX-F(E) | Human Anti-CD22 Recombinant Antibody; Fab Fragment (HPAB-0195-LSX-F(E)) | ELISA | Human Fab |
HPAB-0196-LSX-F(E) | Mouse Anti-CD22 Recombinant Antibody; Fab Fragment (HPAB-0196-LSX-F(E)) | ELISA | Mouse Fab |
HPAB-0197-LSX-F(E) | Human Anti-CD22 Recombinant Antibody; Fab Fragment (HPAB-0197-LSX-F(E)) | ELISA | Human Fab |
HPAB-0198-LSX-F(E) | Mouse Anti-CD22 Recombinant Antibody; Fab Fragment (HPAB-0198-LSX-F(E)) | ELISA, FC | Mouse Fab |
CAT | Product Name | Application | Type |
---|---|---|---|
FAMAB-0046YC | Mouse Anti-CD22 Recombinant Antibody (clone m5/44) | ELISA, Cyt | Mouse IgG1 |
FAMAB-0047YC | Human Anti-CD22 Recombinant Antibody (clone g5/44) | ELISA, Cyt | Humanized IgG4 |
FAMAB-0048YC | Human Anti-CD22 Recombinant Antibody (clone ch5/44) | ELISA | Chimeric (mouse/human) IgG |
HPAB-0709-FY | Mouse Anti-CD22 Recombinant Antibody (clone AB008) | ELISA | Mouse IgG |
HPAB-2052-FY | Human Anti-CD22 Recombinant Antibody (clone B28) | FC | Human IgG1 |
CAT | Product Name | Application | Type |
---|---|---|---|
HPAB-0249-FY-S(P) | Recombinant Human Anti-CD22 Antibody scFv Fragment | F | Human scFv |
HPAB-0034-YJ-S(P) | Mouse Anti-CD22 Recombinant Antibody (clone HB22-23); scFv Fragment | Block | Mouse scFv |
HPAB-0370-FY-S(P) | Human Anti-CD22 Recombinant Antibody; scFv Fragment (HPAB-0370-FY-S(P)) | ELISA | Humanized scFv |
HPAB-0371-FY-S(P) | Human Anti-CD22 Recombinant Antibody; scFv Fragment (HPAB-0371-FY-S(P)) | Inhib | Humanized scFv |
HPAB-2052-FY-S(P) | Human Anti-CD22 Recombinant Antibody (clone B28); scFv Fragment | FC | Human scFv |
CAT | Product Name | Application | Type |
---|---|---|---|
AFC-TAB-176 | Afuco™ Anti-CD22 ADCC Recombinant Antibody (Epratuzumab), ADCC Enhanced | IF, IP, Neut, FuncS, ELISA, FC | ADCC enhanced antibody |
AFC-TAB-198 | Afuco™ Anti-CD22 ADCC Recombinant Antibody (Inotuzumab), ADCC Enhanced | ELISA, FC, IP, FuncS, IF, Neut | ADCC enhanced antibody |
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