Is this antibody suitable for functional assays on human cells?

Yes, this Recombinant Mouse Anti-CD22 Antibody is validated for Functional Studies (FuncS). It targets Human CD22 and can be used to investigate CD22-mediated signaling or internalization in human B-cell models.

What is the host species and isotype of this antibody?

This is a Recombinant Mouse antibody, and the isotype is Mouse IgG. It is produced using recombinant technology to ensure high purity and lot-to-lot consistency.

Mouse Anti-CD22 Recombinant Antibody (HPAB-0201-LSX)

CAT#: HPAB-0201-LSX

This product is a recombinant mouse antibody that recognizes CD22. The antibody was purified by affinity chromatography.

Tested Data Gene Expression

SDS-PAGE

Figure 1 Recombinant Mouse Anti-CD22 Antibody (HPAB-0201-LSX) in SDS-PAGE

SDS-PAGE analysis of HPAB-0201-LSX in non-reduced and β-mercaptoethanol-reduced conditions.

SEC-HPLC

Figure 2 Recombinant Mouse Anti-CD22 Antibody (HPAB-0201-LSX) in HPLC

The purity of HPAB-0201-LSX was greater than 95% as determined by SEC-HPLC.

❮

❯

Normal Tissue Figure 1 Lymph node

Germinal center cells
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/2418/6101_A_7_8.jpg

Normal Tissue Figure 2 Spleen

Cells in red pulp
Staining: Medium
Intensity: Strong
Quantity: <25%
Location: Cytoplasmic/membranous
Cells in white pulp
Staining: High
Intensity: Strong
Quantity: 75%-25%
Location: Cytoplasmic/membranous

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/2418/6101_B_7_4.jpg

RNA Expression Figure 3 RNA cell line category: Cell line enhanced (Daudi, REH, U-698, WM-115)

Cell lines ordered by descending RNA expression order.

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000012124-CD22

❮

❯

Specifications

Immunogen
Human CD22 his-8 tagged extracellular domain lacking domains 3 and 4

Host Species
Mouse

Type
Mouse IgG

Specificity
Human CD22

Species Reactivity
Human

Applications
FuncS, ELISA, WB, FC, RIA

Related Disease
B cell-related disorders include, but are not limited to, malignant lymphoma, multiple myeloma, and chronic lymphocytic leukemia

Product Property

Purity
>95% as determined by SDS-PAGE

Concentration
Please refer to the vial label for the specific concentration.

Buffer
PBS

Preservative
No preservatives

Storage
Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Target

Alternative Names
CD22 Molecule; CD22 Antigen; Sialic Acid Binding Ig-Like Lectin 2; Sialic Acid-Binding Ig-Like Lectin 2; B-Lymphocyte Cell Adhesion Molecule; T-Cell Surface Antigen Leu-14; SIGLEC-2; SIGLEC2; BL-CAM

Gene ID
933

UniProt ID
P20273

REVIEWS AND Q&AS CITATIONS RESOURCES DOWNLOADS RELATED PRODUCTS

Inquiry

Navs

Customer Review

There are currently no Customer reviews or questions for HPAB-0201-LSX. Click the button above to contact us or submit your feedback about this product.

Breast cancer biomarkers at key points during disease progression

Specific B Cell Marker

We used the Anti-CD22 Recombinant Antibody for flow cytometry analysis of B-cell populations. The antibody provided a very specific signal, clearly distinguishing CD22-positive cells from the rest of the PBMC population. The recombinant nature of the product ensured that we had consistent lot-to-lot performance, which was critical for our longitudinal study on B-cell receptor signaling.

Reliable Functional Studies

This antibody performed exceptionally well in our functional assays. We used it to investigate the downstream effects of CD22 ligation on B-cell activation. The results were reproducible and aligned with our hypothesis. The high purity and lack of preservatives in the preparation made it ideal for use in live cell culture experiments without toxicity concerns.

Q&As

Is this antibody suitable for functional assays on human cells?

A: Yes, this Recombinant Mouse Anti-CD22 Antibody is validated for Functional Studies (FuncS). It targets Human CD22 and can be used to investigate CD22-mediated signaling or internalization in human B-cell models.
What is the host species and isotype of this antibody?

A: This is a Recombinant Mouse antibody, and the isotype is Mouse IgG. It is produced using recombinant technology to ensure high purity and lot-to-lot consistency.

View the frequently asked questions answered by Creative Biolabs Support.

Submit Your Publication

Published with our product? Submit your paper and receive a 10% discount on your next order! Share your research to earn exclusive rewards.

Downloadable Resources

Download resources about recombinant antibody development and antibody engineering to boost your research.

Product Notes

This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production

See more details about Hi-Affi™ recombinant antibody benefits.

Datasheet

Datasheet - HPAB-0201-LSX.pdf

MSDS

MSDS - HPAB-0201-LSX.pdf

COA

Certificate of Analysis Lookup

To download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.

Lot Number:

See other products for "CD22"

Select a product category from the dropdown menu below to view related products.

CAT	Product Name	Application	Type
MOB-1248z	Mouse IgG1, kappa Anti-CD22 Recombinant Antibody (MOB-1248z)	IF, FC	Mouse IgG1, κ

CAT	Product Name	Application	Type
MOB-1248z-F(E)	Recombinant Anti-Human CD22 Antibody Fab Fragment	IP, RIA, FuncS	Fab

CAT	Product Name	Application	Type
MOB-1248z-S(P)	Recombinant Anti-Human CD22 Antibody scFv Fragment	ELISA, WB, IF, FuncS	scFv

CAT	Product Name	Application	Type
TAB-176	Anti-Human CD22 Recombinant Antibody (TAB-176)	IF, IP, Neut, FuncS, ELISA, FC, ICC	IgG1

CAT	Product Name	Application	Type
TAB-183-F(E)	Anti-Human CD22 Recombinant Antibody (TAB-183-F(E))	FuncS, IF, Neut, ELISA, FC, IP, ICC	Fv - disulfide stabilized

CAT	Product Name	Application	Type
TAB-198	Humanized Anti-CD22 Recombinant Antibody (clone Inotuzumab)	ELISA, FC, IP, FuncS, IF, Neut, ICC	Humanized (from mouse) IgG4, κ

CAT	Product Name	Application	Type
TAB-047-F(E)	Anti-Human CD22 Recombinant Antibody Fab Fragment(Bectumomab)	ELISA, FC, IP, FuncS, IF, Neut, WB	Fab' - G2a - kappa

CAT	Product Name	Application	Type
TAB-899	Humanized Anti-CD22 Recombinant Antibody (clone Pinatuzumab)	FC, IP, ELISA, Neut, FuncS, IF, ICC	Humanized (from mouse) IgG1, κ

CAT	Product Name	Application	Type
AGTO-G077E	Anti-CD22 immunotoxin RFB4 (IgG)-PE	Cytotoxicity assay, Function study

CAT	Product Name	Application	Type
AGTO-G077D	Anti-CD22 immunotoxin RFB4 (IgG)-DT	Cytotoxicity assay, Function study

CAT	Product Name	Application	Type
AGTO-G077G	Anti-CD22 immunotoxin RFB4 (IgG)-Gel	Cytotoxicity assay, Function study

CAT	Product Name	Application	Type
AGTO-G077R	Anti-CD22 immunotoxin RFB4 (IgG)-RTA	Cytotoxicity assay, Function study

CAT	Product Name	Application	Type
AGTO-G077S	Anti-CD22 immunotoxin RFB4 (IgG)-Sap	Cytotoxicity assay, Function study

CAT	Product Name	Application	Type
TAB-0196CL	Anti-Human CD22 Recombinant Antibody (hLL2)	Inhib, Cyt, FC, WB

CAT	Product Name	Application	Type
TAB-0197CL	Anti-Human CD22 Recombinant Antibody (hLL2-Cys)	ELISA, In vivo

CAT	Product Name	Application	Type
TAB-1714CL	Mouse Anti-CD22 Recombinant Antibody (TAB-1714CL)	ELISA, WB	Mouse IgG

CAT	Product Name	Application	Type
TAB-1715CL	Human Anti-CD22 Recombinant Antibody (TAB-1715CL)	Cyt, FuncS	Fully human antibody

CAT	Product Name	Application	Type
TAB-1716CL	Human Anti-CD22 Recombinant Antibody (TAB-1716CL)	Cyt, FuncS	Fully human antibody

CAT	Product Name	Application	Type
TAB-1717CL	Human Anti-CD22 Recombinant Antibody (TAB-1717CL)	Cyt, FuncS	Fully human antibody

CAT	Product Name	Application	Type
TAB-1724CL	Human Anti-CD22 Recombinant Antibody (TAB-1724CL)	Inhib	Chimeric (mouse/human) IgG1, κ

CAT	Product Name	Application	Type
TAB-1725CL	Human Anti-CD22 Recombinant Antibody (TAB-1725CL)	Block, Cyt, ELISA, FuncS	Humanized IgG

CAT	Product Name	Application	Type
TAB-1728CL	Anti-Human CD22 Recombinant Antibody (RFB4)	IF, FC

CAT	Product Name	Application	Type
TAB-1729CL	Human Anti-CD22 Recombinant Antibody (TAB-1729CL)	IF, FC	Humanized IgG

CAT	Product Name	Application	Type
TAB-1714CL-S(P)	Mouse Anti-CD22 Recombinant Antibody; scFv Fragment (TAB-1714CL-S(P))	ELISA, WB	Mouse scFv

CAT	Product Name	Application	Type
TAB-1724CL-S(P)	Mouse Anti-CD22 Recombinant Antibody; scFv Fragment (TAB-1724CL-S(P))	Inhib	Mouse scFv

CAT	Product Name	Application	Type
TAB-1724CL-F(E)	Human Anti-CD22 Recombinant Antibody; Fab Fragment (TAB-1724CL-F(E))	Inhib	Chimeric (mouse/human) Fab

CAT	Product Name	Application	Type
TAB-001LC	Human Anti-CD22 Recombinant Antibody (TAB-001LC)	ELISA	Chimeric antibody (mouse/human)

CAT	Product Name	Application	Type
TAB-001LC-S(P)	Mouse Anti-CD22 Recombinant Antibody; scFv Fragment (TAB-001LC-S(P))	ELISA	Mouse scFv

CAT	Product Name	Application	Type
Gly-001LC	Recombinant Anti-Human CD22 Antibody (Fab glycosylation)	ELISA, FC	Mouse antibody

CAT	Product Name	Application	Type
Gly-111LC	Recombinant Anti-Human CD22 Antibody (Fc glycosylation)	ELISA	Chimeric antibody (mouse/human)

CAT	Product Name	Application	Type
MOB-0349MZ	Mouse Anti-CD22 Recombinant Antibody (clone 5LB129)	ELISA	Mouse IgG1

CAT	Product Name	Application	Type
NEUT-315CQ	Rat Anti-CD22 Recombinant Antibody (clone 2D6)	Block	Rat IgG1, κ

CAT	Product Name	Application	Type
MOR-0545	Hi-Affi™ Rabbit Anti-CD22 Recombinant Antibody (clone DS545AB)	IP, WB	Rabbit IgG

CAT	Product Name	Application	Type
HPAB-0195-LSX	Human Anti-CD22 Recombinant Antibody (HPAB-0195-LSX)	Apop, Cyt, FC, WB, Inhib, FuncS, IF, ELISA	Human IgG

CAT	Product Name	Application	Type
HPAB-0196-LSX	Mouse Anti-CD22 Recombinant Antibody (HPAB-0196-LSX)	ELISA, FC, IP, RIA	Mouse IgG

CAT	Product Name	Application	Type
HPAB-0197-LSX	Human Anti-CD22 Recombinant Antibody (HPAB-0197-LSX)	ELISA, FC, FuncS	Human IgG

CAT	Product Name	Application	Type
HPAB-0194-LSX-S(P)	Mouse Anti-CD22 Recombinant Antibody; scFv Fragment (HPAB-0194-LSX-S(P))	ELISA	Mouse scFv

CAT	Product Name	Application	Type
HPAB-0195-LSX-S(P)	Human Anti-CD22 Recombinant Antibody; scFv Fragment (HPAB-0195-LSX-S(P))	ELISA	Human scFv

CAT	Product Name	Application	Type
HPAB-0196-LSX-S(P)	Mouse Anti-CD22 Recombinant Antibody; scFv Fragment (HPAB-0196-LSX-S(P))	ELISA	Mouse scFv

CAT	Product Name	Application	Type
HPAB-0197-LSX-S(P)	Human Anti-CD22 Recombinant Antibody; scFv Fragment (HPAB-0197-LSX-S(P))	ELISA	Human scFv

CAT	Product Name	Application	Type
HPAB-0194-LSX-F(E)	Mouse Anti-CD22 Recombinant Antibody; Fab Fragment (HPAB-0194-LSX-F(E))	ELISA	Mouse Fab

CAT	Product Name	Application	Type
HPAB-0195-LSX-F(E)	Human Anti-CD22 Recombinant Antibody; Fab Fragment (HPAB-0195-LSX-F(E))	ELISA	Human Fab

CAT	Product Name	Application	Type
HPAB-0196-LSX-F(E)	Mouse Anti-CD22 Recombinant Antibody; Fab Fragment (HPAB-0196-LSX-F(E))	ELISA	Mouse Fab

CAT	Product Name	Application	Type
HPAB-0197-LSX-F(E)	Human Anti-CD22 Recombinant Antibody; Fab Fragment (HPAB-0197-LSX-F(E))	ELISA	Human Fab

CAT	Product Name	Application	Type
AFC-TAB-176	Afuco™ Anti-CD22 ADCC Recombinant Antibody, ADCC Enhanced (AFC-TAB-176)	IF, IP, Neut, FuncS, ELISA, FC	ADCC enhanced antibody

CAT	Product Name	Application	Type
AFC-TAB-198	Afuco™ Anti-CD22 ADCC Recombinant Antibody, ADCC Enhanced (AFC-TAB-198)	ELISA, FC, IP, FuncS, IF, Neut	ADCC enhanced antibody

CAT	Product Name	Application	Type
VS-0424-XY50	AbPlus™ Anti-CD22 Magnetic Beads (DM13)	IP, Protein Purification

CAT	Product Name	Application	Type
VS-0724-YC918	AbPlus™ Anti-Cd22 Magnetic Beads (VS-0724-YC918)	IP, Protein Purification

CAT	Product Name	Application	Type
VS-0225-XY24	CytoStream™ Mouse Anti-CD22 Recombinant Antibody (VS-0225-XY24)	FC	Mouse IgG1, kappa

CAT	Product Name	Application	Type
VS-0325-XY357	Anti-CD22 Immunohistochemistry Kit	IHC

CAT	Product Name	Application	Type
VS-0425-FY82	Human Anti-CD22 (clone Inotuzumab) scFv-Fc Chimera	ELISA	Human IgG4, scFv-Fc

CAT	Product Name	Application	Type
VS-0425-YC225	Recombinant Anti-CD22 Vesicular Antibody, EV Displayed (VS-0425-YC225)	ELISA, FC, Neut, Cell-uptake

CAT	Product Name	Application	Type
VS-0525-YC201	Recombinant Anti-CD22 (Epitope A x Epitope B) Biparatopic Antibody, Tandem scFv	ELISA, FC, ICC, IF, IP, Neut	Tandem scFv

CAT	Product Name	Application	Type
VS-0525-XY1143	Anti-Human CD22 Immunohistochemistry Kit	IHC

CAT	Product Name	Application	Type
VS-0825-YC43	SmartAb™ Recombinant Anti-CD22 pH-dependent Antibody (Clone Inotuzumab)	ELISA, FC, IP IF, Neut, ICC	Human IgG4 kappa

CAT	Product Name	Application	Type
VS-1025-YC100	Anti-CD22 Antibody Prodrug, Protease Activated (VS-1025-YC100)	ISZ, Cyt, FuncS

CAT	Product Name	Application	Type
VS-1125-XY17	Rabbit Anti-CD22 Recombinant Antibody (VS-1125-XY17)	FC	Rabbit IgG

CAT	Product Name	Application	Type
VS-1125-XY18	Rabbit Anti-CD22 Recombinant Antibody (VS-1125-XY18)	WB, IHC, IP	Rabbit IgG

CAT	Product Name	Application	Type
VS-1125-XY19	Rat Anti-CD22 Recombinant Antibody (VS-1125-XY19)	FC	Rat IgG1, kappa

CAT	Product Name	Application	Type
VS-1125-XY20	Rat Anti-CD22 Recombinant Antibody (VS-1125-XY20)	WB, FC	Rat IgG2a

CAT	Product Name	Application	Type
VS-1125-XY21	Rabbit Anti-CD22 Recombinant Antibody (VS-1125-XY21)	WB, IP	Rabbit IgG

Specific Inquiry

Size:

Conjugation:

Endotoxin:

Purity:

Formats:

Isotype Switching:

Fc Engineering:

Modalities:

Add to compare

Request
Custom Production

See Our Custom Production in Action

Figure 1 Anti-HBV Recombinant Antibody (TAB-147) in SDS-PAGE

Download the full case study

Popular Products

Anti-ANGPT2 Recombinant Antibody (CAT# TAB-769)
Application: IF, IP, Neut, FuncS, ELISA, FC, ICC

Anti-CD248 Recombinant Antibody (Ontuxizumab) (CAT# TAB-773)
Application: Neut, ELISA, IF, IP, FuncS, FC, ICC

Human Anti-IGF1R Recombinant Antibody (TAB-199) (CAT# TAB-199)
Application: FC, IP, ELISA, Neut, FuncS, IF, ICC

Human Anti-IL17RA Recombinant Antibody (TAB-134) (CAT# TAB-134)
Application: Neut, ELISA, IF, IP, FuncS, FC, ICC

Human Anti-NECTIN4 Recombinant Antibody (TAB-H25) (CAT# TAB-H25)
Application: ELISA, Neut, IF, IP, FC, FuncS

Chimeric (Mouse/Human) Anti-SDC1 Recombinant Antibody (TAB-197) (CAT# TAB-197)
Application: Neut, ELISA, IF, IP, FuncS, FC, ICC

Mouse Anti-CD5 Recombinant Antibody (clone Zolimomab) (CAT# TAB-884)
Application: IP, IF, FuncS, FC, Neut, ELISA, ICC

Anti-Human Integrin alpha 5 beta 1 Recombinant Antibody (Volociximab) (CAT# TAB-077)
Application: ELISA, IHC

Anti-Human CD30 Recombinant Antibody (Iratumumab) (CAT# TAB-144)
Application: ELISA, FC, IP, FuncS, IF, Neut, ICC

Anti-Human NOTCH2 Recombinant Antibody (TAB-H67) (CAT# TAB-H67)
Application: FuncS, Block, ELISA

Anti-EGFR Recombinant Antibody (Imgatuzumab) (CAT# TAB-753)
Application: Neut, ELISA, IF, IP, FuncS, FC, WB

Human Anti-SLC34A2 Recombinant Antibody (TAB-H45) (CAT# TAB-H45)
Application: ELISA, FC, IP, FuncS, IF, Neut, ICC

Anti-Human ERBB2 Recombinant Antibody (TAB-053) (CAT# TAB-053)
Application: FuncS, IF, Neut, ELISA, FC, IP, WB

Anti-Human IL4 Recombinant Antibody (TAB-221) (CAT# TAB-221)
Application: WB, ELISA, FC, IP, FuncS, IF, Neut

Anti-Human CD19 Recombinant Antibody (TAB-108CL) (CAT# TAB-108CL)
Application: FC, ADCC, FuncS

Anti-Human CTGF Recombinant Antibody (TAB-194CL) (CAT# TAB-194CL)
Application: WB, IHC, IF

Anti-Human IL21R Recombinant Antibody (PF-05230900) (CAT# TAB-416CL)
Application:

Anti-Human MET Recombinant Antibody (TAB-521CL) (CAT# TAB-521CL)
Application: WB, FuncS

Recombinant Human Anti-RSV Antibody (AM14) (CAT# PABL-321)
Application: WB, ELISA, Neut, FuncS

Recombinant Mouse Anti-DENV-3 Antibody (mAb 4E11) (CAT# PABL-446)
Application: WB, FuncS

For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.

Send Inquiry

This site is protected by reCAPTCHA and the Google Privacy Policy and Terms of Service apply.