Human Anti-CD274 Recombinant Antibody; scFv Fragment (HPAB-0196-YC-S(P)) (CAT#: HPAB-0196-YC-S(P))
This product is a scFv fragment antibody binding to human programmed death-ligand 1 (PD-L1, also known as CD274). With a significantly higher affinity and specificity for PD-Ll, this antibody demonstrated some cross-reactivity with PD-L2. This scFv antibody was originally selected from a large phage display library screened on the recombinant CD274 protein of human origin. This scFv antibody has a significant inhibition of hPD-1/hPD-L1 binding. It is purified by affinity chromatography to get a purity higher than 95% and could be applied in ELISA, radioimmunoassay, and flow cytometry.
Specific Inquiry
Subcellular Location and Protein Expression
Normal Tissue
RNA Expression
Figure 1 IF staining of human cell line U-251 MG
(Immunofluorescent staining of human cell line U-251 MG shows localization to plasma membrane.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/3528/1899_B9_6_cr5ba2049576272_selected.jpg
(Immunofluorescent staining of human cell line U-251 MG shows localization to plasma membrane.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/3528/1899_B9_6_cr5ba2049576272_selected.jpg
Figure 2 IF staining of human cell line BJ
(Immunofluorescent staining of human cell line BJ shows localization to plasma membrane.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/3528/1936_G3_3_blue_red_green.jpg
(Immunofluorescent staining of human cell line BJ shows localization to plasma membrane.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/3528/1936_G3_3_blue_red_green.jpg
Figure 3 IF staining of human cell line U-2 OS
(Immunofluorescent staining of human cell line U-2 OS shows localization to plasma membrane.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/3528/1953_G5_3_blue_red_green.jpg
(Immunofluorescent staining of human cell line U-2 OS shows localization to plasma membrane.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/3528/1953_G5_3_blue_red_green.jpg
Figure 4 Colon
(Peripheral nerve/ganglion
Staining:High
Intensity: Strong
Quantity: 75%-25%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/76385/169546_A_9_3.jpg
(Peripheral nerve/ganglion
Staining:High
Intensity: Strong
Quantity: 75%-25%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/76385/169546_A_9_3.jpg
Figure 5 Placenta
(Cytotrophoblasts
Staining:Medium
Intensity: Moderate
Quantity: 75%-25%
Syncytiotrophoblasts - cell body
Staining:Medium
Intensity: Moderate
Quantity:>75%
Syncytiotrophoblasts - microvilli
Staining:High
Intensity: Strong)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/76385/169546_A_1_7.jpg
(Cytotrophoblasts
Staining:Medium
Intensity: Moderate
Quantity: 75%-25%
Syncytiotrophoblasts - cell body
Staining:Medium
Intensity: Moderate
Quantity:>75%
Syncytiotrophoblasts - microvilli
Staining:High
Intensity: Strong)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/76385/169546_A_1_7.jpg
Figure 6 RNA cell line category: Cell line enhanced (HDLM-2, HHSteC, U-251 MG)
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000120217-CD274
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000120217-CD274
Specifications
- Host Species
- Human
- Type
- Human scFv
- Specificity
- Human CD274
- Species Reactivity
- Human
- Applications
- FC
- Related Disease
- Cancer, Chronic viral infection
Product Property
- Purity
- >95% as determined by SDS-PAGE
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- PBS
- Preservative
- No preservatives
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Target
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
Download resources about recombinant antibody development and antibody engineering to boost your research.
See other products for "Cd274"
Select a product category from the dropdown menu below to view related products.
Please select product type
IgG Antibody Products
ScFv Antibody Products
Humanized Antibody Products
Chimeric Antibody Products
Human Antibody Products
Mouse Antibody Products
Neutralizing Antibody Products
Blocking Antibody Products
Rabbit Monoclonal Antibody Products
Single Domain Antibody Products
Fab Antibody Products
ADCC Enhanced Antibody Products
Hexamer Antibody Products
Non-Human Primate (NHP) Antibody Products
Flow Cytometry (FC) related Products
ScFv-Fc Chimera Products
| CAT | Product Name | Application | Type |
|---|---|---|---|
| PABL-298 | Human Anti-CD274 Recombinant Antibody (clone CBLC-006) | WB, ELISA, FuncS | Human IgG |
| HPAB-N0326-YC | Human Anti-CD274 Recombinant Antibody (HPAB-N0326-YC) | ELISA, FuncS | Human IgG |
| HPAB-N0407-YC | Human Anti-CD274 Recombinant Antibody; VHH-Fc Fragment (HPAB-N0407-YC) | FuncS, ELISA, Block | Human VHH-Fc |
| MRO-1200-CN | Rabbit Anti-CD274 Recombinant Antibody (clone CBACN-439) | WB, IHC | Rabbit IgG |
| HPAB-0114-YJ | Human Anti-CD274 Recombinant Antibody (HPAB-0114-YJ) | Block, ELISA, FC, Neut, FuncS | Human IgG1 |
Customer Reviews and Q&As
There are currently no Customer reviews or questions for HPAB-0196-YC-S(P). Click the button above to contact us or submit your feedback about this product.
Popular products with customers
Application: ELISA, IP, FC, FuncS, Neut, IF, ICC
Application: ELISA, FC, IP, FuncS, IF, Neut, ICC
Application: IP, IF, FuncS, FC, Neut, ELISA, ICC
-2.png)
Application: Neut, ELISA, IF, IP, FuncS, FC, ICC
Application: WB, Neut, FuncS
Application: ELISA, FC, WB, FuncS
Application: ELISA, FC, IF, IHC, WB
Application: ELISA, IF, Block, FuncS
-1.jpg)
Application: FC, FuncS, IA, IF, IP, IHC
Application: ELISA, Cyt, PP, Inhib
For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
Send Inquiry
This site is protected by reCAPTCHA and the Google Privacy Policy and Terms of Service apply.
