Human Anti-CD274 Recombinant Antibody (TAB-0263CL) (CAT#: TAB-0263CL)
This product is a recombinant human antibody that recognizes PD-L1. The binding ability of this antibody was analyzed by the incubation with HEK293/PD-L1 cells and the EC₅₀ value was around 0.1 μg/mL as detected by flow cytometry. This antibody could stimulate cytokine production of activated PBMC cells as the IFN-γ secretion tested by ELISA. This human antibody could be constructed and produced in Fab or scFv formats and purified by affinity chromatography with a purity higher than 95%.
Specific Inquiry
Subcellular Location and Protein Expression
Normal Tissue
RNA Expression
Figure 1 IF staining of human cell line U-251 MG
(Immunofluorescent staining of human cell line U-251 MG shows localization to plasma membrane.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/3528/1899_B9_6_cr5ba2049576272_selected.jpg
(Immunofluorescent staining of human cell line U-251 MG shows localization to plasma membrane.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/3528/1899_B9_6_cr5ba2049576272_selected.jpg
Figure 2 IF staining of human cell line BJ
(Immunofluorescent staining of human cell line BJ shows localization to plasma membrane.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/3528/1936_G3_3_blue_red_green.jpg
(Immunofluorescent staining of human cell line BJ shows localization to plasma membrane.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/3528/1936_G3_3_blue_red_green.jpg
Figure 3 IF staining of human cell line U-2 OS
(Immunofluorescent staining of human cell line U-2 OS shows localization to plasma membrane.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/3528/1953_G5_3_blue_red_green.jpg
(Immunofluorescent staining of human cell line U-2 OS shows localization to plasma membrane.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/3528/1953_G5_3_blue_red_green.jpg
Figure 4 Colon
(Peripheral nerve/ganglion
Staining:High
Intensity: Strong
Quantity: 75%-25%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/76385/169546_A_9_3.jpg
(Peripheral nerve/ganglion
Staining:High
Intensity: Strong
Quantity: 75%-25%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/76385/169546_A_9_3.jpg
Figure 5 Placenta
(Cytotrophoblasts
Staining:Medium
Intensity: Moderate
Quantity: 75%-25%
Syncytiotrophoblasts - cell body
Staining:Medium
Intensity: Moderate
Quantity:>75%
Syncytiotrophoblasts - microvilli
Staining:High
Intensity: Strong)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/76385/169546_A_1_7.jpg
(Cytotrophoblasts
Staining:Medium
Intensity: Moderate
Quantity: 75%-25%
Syncytiotrophoblasts - cell body
Staining:Medium
Intensity: Moderate
Quantity:>75%
Syncytiotrophoblasts - microvilli
Staining:High
Intensity: Strong)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/76385/169546_A_1_7.jpg
Figure 6 RNA cell line category: Cell line enhanced (HDLM-2, HHSteC, U-251 MG)
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000120217-CD274
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000120217-CD274
Specifications
- Host Species
- Human
- Type
- Humanized IgG
- Specificity
- Human
- Species Reactivity
- Human
- Applications
- ELISA, WB, SPR, FuncS
- Related Disease
- Cancer, infectious diseases, pathological disorders
Product Property
- Purity
- >95% as determined by SDS-PAGE
- Buffer
- PBS
- Preservative
- No preservatives
- Storage
- Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Target
- Alternative Names
- CD274; CD274 molecule; B7-H; B7H1; PDL1; PD-L1; PDCD1L1; PDCD1LG1; programmed cell death 1 ligand 1; B7 homolog 1; CD274 antigen; PDCD1 ligand 1; programmed death ligand 1
- Gene ID
- 29126
- UniProt ID
- Q0GN75
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
Download resources about recombinant antibody development and antibody engineering to boost your research.
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Flow Cytometry (FC) related Products
ScFv-Fc Chimera Products
| CAT | Product Name | Application | Type |
|---|---|---|---|
| PSBL-298 | Human Anti-CD274 Recombinant Antibody (clone CBLC-006); scFv Fragment | WB, ELISA, FuncS | Human scFv |
| HPAB-1440LY-S(P) | Rabbit Anti-CD274 Recombinant Antibody scFv Fragment (HPAB-1440LY-S(P)) | IHC | Rabbit scFv |
| HPAB-0400LY-S(P) | Human Anti-CD274 Recombinant Antibody; scFv Fragment (HPAB-0400LY-S(P)) | ELISA, RIA, WB | Human scfv |
| HPAB-0401LY-S(P) | Human Anti-CD274 Recombinant Antibody; scFv Fragment (HPAB-0401LY-S(P)) | ELISA, RIA, WB | Human scfv |
| HPAB-0402LY-S(P) | Human Anti-CD274 Recombinant Antibody; scFv Fragment (HPAB-0402LY-S(P)) | ELISA, RIA, WB | Human scfv |
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For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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