Rabbit Anti-CD276 Recombinant Antibody (HPAB-0244-CN) (CAT#: HPAB-0244-CN)
This product is a recombinant rabbit IgG antibody that binds human B7-H3. The antibody can be used in WB and ELISA assay. The antibody detected a 110 kDa band by Western blotting. The EC50 of the antibody was 67 pM, demonstrating the high potency of this antibody binding to the B7-H3 epitope.
Specific Inquiry
Subcellular Location
Normal Tissue
RNA Expression
Figure 1 IF staining of human cell line A-431
(Immunofluorescent staining of human cell line A-431 shows localization to vesicles.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/17139/if_selected.jpg
(Immunofluorescent staining of human cell line A-431 shows localization to vesicles.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/17139/if_selected.jpg
Figure 2 Placenta.
(High expression in placenta)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/17826/40943_A_1_7_val_selected.jpg
(High expression in placenta)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/17826/40943_A_1_7_val_selected.jpg
Figure 3 Testis
(Cells in seminiferous ducts Staining: Low Intensity: Weak Quantity: 75%-25% Location: Cytoplasmic/ membranous Leydig cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/9285/25731_A_5_6.jpg
(Cells in seminiferous ducts Staining: Low Intensity: Weak Quantity: 75%-25% Location: Cytoplasmic/ membranous Leydig cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/9285/25731_A_5_6.jpg
Figure 4 Cerebral cortex
(Neuropil Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/9285/25731_B_7_5.jpg
(Neuropil Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/9285/25731_B_7_5.jpg
Figure 5 Colon
(Endothelial cells Staining: Low Intensity: Weak Quantity:>75% Location: Cytoplasmic/ membranous Glandular cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/9285/25731_A_9_3.jpg
(Endothelial cells Staining: Low Intensity: Weak Quantity:>75% Location: Cytoplasmic/ membranous Glandular cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/9285/25731_A_9_3.jpg
Figure 6 Liver
(Cholangiocytes Staining: Low Intensity: Weak Quantity:>75% Location: Cytoplasmic/ membranous Hepatocytes Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/9285/25731_A_7_4.jpg
(Cholangiocytes Staining: Low Intensity: Weak Quantity:>75% Location: Cytoplasmic/ membranous Hepatocytes Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/9285/25731_A_7_4.jpg
Figure 7 RNA cell line category: Cell line enhanced (HaCaT, U-2197)
(Cell lines ordered by descending RNA expression order)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000103855-CD276
(Cell lines ordered by descending RNA expression order)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000103855-CD276
Specifications
- Immunogen
- aa 521-534 of human B7-H3
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human CD276
- Species Reactivity
- Human
- Applications
- WB, ELISA, FC, IHC, ICC
- Related Disease
- Tumor
Product Property
- Purity
- >95% as determined by SDS-PAGE
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- PBS
- Preservative
- No preservatives
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- Western blot: SP265 detected a 110 kDa band by Western blotting. This band was detected in Hs700t pancreatic cancer cells (which are known to exhibit elevated levels of B7-H3), and was absent in the negative control Raji cells. Immunohistochemistry: 0.3 μg/mL
Target
- Alternative Names
- CD276 Molecule; Costimulatory Molecule; CD276 Antigen; B7 Homolog 3; 4Ig-B7-H3; B7-H3; B7H3; B7RP-2
- Gene ID
- 80381
- UniProt ID
- Q5ZPR3
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
Download resources about recombinant antibody development and antibody engineering to boost your research.
See other products for "CD276"
Humanized Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-117CL | Anti-Human CD276 Recombinant Antibody (MGA271) | ADCC, FuncS | Antibody |
| TAB-424CQ | Anti-Human CD276 Recombinant Antibody (Enoblituzumab) | ELISA, IHC, FC, IP, IF, FuncS | IgG1, κ |
Recombinant Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-1603MZ | Mouse Anti-CD276 Recombinant Antibody (clone NJI53) | FC | Mouse IgG1 |
| HPAB-0241-CN | Human Anti-CD276 Recombinant Antibody (HPAB-0241-CN) | ELISA, FC, IP | Chimeric (mouse/human) IgG1, κ |
| HPAB-0048LY | Mouse Anti-CD276 Recombinant Antibody (HPAB-0048LY) | FuncS, ELISA, FC, IHC | Mouse IgG |
| HPAB-0049LY | Human Anti-CD276 Recombinant Antibody (HPAB-0049LY) | FuncS, ELISA, FC, IHC | Humanized IgG |
| VS3-WK1032 | Mouse Anti-CD276 Recombinant Antibody (VS3-WK1032) | IHC-P, WB | Mouse IgG1, κ |
Blocking Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| NEUT-333CQ | Rat Anti-CD276 Recombinant Antibody (clone MJ18) | Block, FC | Rat IgG1 |
Rabbit Monoclonal Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-0549 | Hi-Affi™ Rabbit Anti-CD276 Recombinant Antibody (clone DS549AB) | WB, IP, FC | Rabbit IgG |
scFv Fragment Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-0234-CN-S(P) | Human Anti-CD276 Recombinant Antibody; scFv Fragment (HPAB-0234-CN-S(P)) | ELISA, FC, IF | Human scFv |
| HPAB-0236-CN-S(P) | Mouse Anti-CD276 Recombinant Antibody; scFv Fragment (HPAB-0236-CN-S(P)) | ELISA, FC, IHC | Mouse scFv |
| HPAB-0048LY-S(P) | Mouse Anti-CD276 Recombinant Antibody; scFv Fragment (HPAB-0048LY-S(P)) | FuncS, ELISA, FC, IHC | Mouse scFv |
| HPAB-0049LY-S(P) | Human Anti-CD276 Recombinant Antibody; scFv Fragment (HPAB-0049LY-S(P)) | FuncS, ELISA, FC, IHC | Human scFv |
| HPAB-0373-FY-S(P) | Human Anti-CD276 Recombinant Antibody; scFv Fragment (HPAB-0373-FY-S(P)) | ELISA | Human scFv |
Fab Fragment Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-0234-CN-F(E) | Human Anti-CD276 Recombinant Antibody; Fab Fragment (HPAB-0234-CN-F(E)) | ELISA, FC, IF | Human Fab |
| HPAB-0236-CN-F(E) | Mouse Anti-CD276 Recombinant Antibody; Fab Fragment (HPAB-0236-CN-F(E)) | ELISA, FC, IHC | Mouse Fab |
| HPAB-0237-CN-F(E) | Mouse Anti-CD276 Recombinant Antibody; Fab Fragment (HPAB-0237-CN-F(E)) | ELISA, FC, IHC | Mouse Fab |
| HPAB-0238-CN-F(E) | Mouse Anti-CD276 Recombinant Antibody; Fab Fragment (HPAB-0238-CN-F(E)) | ELISA, FC, IHC | Mouse Fab |
| HPAB-0239-CN-F(E) | Human Anti-CD276 Recombinant Antibody; Fab Fragment (HPAB-0239-CN-F(E)) | ELISA, FC, IHC | Humanized Fab |
ADCC Enhanced Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| AFC-TAB-424CQ | Afuco™ Anti-CD276 ADCC Recombinant Antibody (Enoblituzumab), ADCC Enhanced | ELISA, IHC, FC, IP, IF, FuncS | ADCC enhanced antibody |
Customer Reviews and Q&As
There are currently no Customer reviews or questions for HPAB-0244-CN. Click the button above to contact us or submit your feedback about this product.
View the frequently asked questions answered by Creative Biolabs Support.
For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
Send Inquiry
This site is protected by reCAPTCHA and the Google Privacy Policy and Terms of Service apply.