Mouse Anti-CD276 Recombinant Antibody (HPAB-0236-CN) (CAT#: HPAB-0236-CN)
This HPAB-0236-CN product is a recombinant mouse IgG1, κ antibody that binds human B7-H3. This clone, generated from hybridoma, can be used for IHC studies in samples of pancreatic cancer, breast cancer, colon, and lung tissues. This antibody showed negative staining in liver and pancreas at up to 5 μg/ml.
Specific Inquiry
ELISA
Figure 1 Anti-CD276 Antibody (HPAB-0236-CN) in ELISA
ELISA analysis of HPAB-0236-CN was performed by coating with B7-H3 Protein, Human, Recombinant (His Tag).
The secondary antibody: HRP-Anti-Mouse IgG (H+L)
WB
Figure 2 Anti-CD276 Antibody (HPAB-0236-CN) in WB
Western blot analysis of HPAB-0236-CN was performed with B7-H3 Protein, Human, Recombinant (His Tag).
HPAB-0236-CN incubation concentration: 2ng/μL.
The secondary antibody: HRP-Anti-Mouse IgG (H+L)
Lane 1: Reducing antigen (0.1μg)
Lane 2: Reducing antigen (0.3μg)
Lane 3: Reducing antigen (0.6μg)
DB
Figure 3 Anti-CD276 Antibody (HPAB-0236-CN) in DB
Dot blot analysis of HPAB-0236-CN was performed with B7-H3 Protein, Human, Recombinant (His Tag).
HPAB-0236-CN incubation concentration: 2ng/μL.
NC: Negative control PC: Positive control
Specifications
- Immunogen
- These antibodies are produced by immunizing mice with immunogenic amounts of cells, cell extracts, or protein preparations containing human B7-H3.
- Host Species
- Mouse
- Type
- Mouse IgG1, κ
- Specificity
- Human CD276
- Species Reactivity
- Human, Cynomolgus
- Applications
- IHC, Cyt, Inhib, ELISA, FC
- Related Disease
- Cancer and inflammation
Product Property
- Purity
- >95% as determined by SDS-PAGE
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- PBS
- Preservative
- No preservatives
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- A549 lung cancer cells were incubated in the presence of hBRCA84D, chBRCA84D, and hBRCA84 (0264 Fc) variants, and the cytotoxic effects of these antibodies were determined. This indicates that all three antibodies are cytotoxic to A549 cells. Immunohistochemistry: 0.078 μg/mL-0.625 μg/mL
Target
- Alternative Names
- CD276 Molecule; Costimulatory Molecule; CD276 Antigen; B7 Homolog 3; 4Ig-B7-H3; B7-H3; B7H3; B7RP-2
- Gene ID
- 80381
- UniProt ID
- Q5ZPR3
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
Download resources about recombinant antibody development and antibody engineering to boost your research.
See other products for "CD276"
Humanized Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-117CL | Anti-Human CD276 Recombinant Antibody (MGA271) | ADCC, FuncS | Antibody |
| TAB-424CQ | Anti-Human CD276 Recombinant Antibody (Enoblituzumab) | ELISA, IHC, FC, IP, IF, FuncS | IgG1, κ |
Blocking Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| NEUT-333CQ | Rat Anti-CD276 Recombinant Antibody (clone MJ18) | Block, FC | Rat IgG1 |
Rabbit Monoclonal Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-0549 | Hi-Affi™ Rabbit Anti-CD276 Recombinant Antibody (clone DS549AB) | WB, IP, FC | Rabbit IgG |
scFv Fragment Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-0048LY-S(P) | Mouse Anti-CD276 Recombinant Antibody; scFv Fragment (HPAB-0048LY-S(P)) | FuncS, ELISA, FC, IHC | Mouse scFv |
| HPAB-0049LY-S(P) | Human Anti-CD276 Recombinant Antibody; scFv Fragment (HPAB-0049LY-S(P)) | FuncS, ELISA, FC, IHC | Human scFv |
| HPAB-M0422-YC-S(P) | Mouse Anti-CD276 Recombinant Antibody; scFv Fragment (HPAB-M0422-YC-S(P)) | Cyt, FuncS | Mouse scFv |
| HPAB-M0423-YC-S(P) | Mouse Anti-CD276 Recombinant Antibody; scFv Fragment (HPAB-M0423-YC-S(P)) | Cyt, FuncS | Mouse scFv |
| HPAB-0373-FY-S(P) | Human Anti-CD276 Recombinant Antibody; scFv Fragment (HPAB-0373-FY-S(P)) | ELISA | Human scFv |
Recombinant Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MRO-1743-CN | Rabbit Anti-CD276 Polyclonal Antibody (MRO-1743-CN) | WB, IF, IHC, FC | Rabbit IgG |
| HPAB-M0422-YC | Mouse Anti-CD276 Recombinant Antibody (HPAB-M0422-YC) | Cyt, FuncS | Mouse IgG |
| HPAB-M0423-YC | Mouse Anti-CD276 Recombinant Antibody (HPAB-M0423-YC) | Cyt, FuncS | Mouse IgG |
| HPAB-M0424-YC | Human Anti-CD276 Recombinant Antibody (HPAB-M0424-YC) | Cyt, FuncS, IHC | Humanized IgG |
| HPAB-M0425-YC | Mouse Anti-CD276 Recombinant Antibody (HPAB-M0425-YC) | Cyt, FuncS | Mouse IgG |
Fab Fragment Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-M0422-YC-F(E) | Mouse Anti-CD276 Recombinant Antibody; Fab Fragment (HPAB-M0422-YC-F(E)) | Cyt, FuncS | Mouse Fab |
| HPAB-M0423-YC-F(E) | Mouse Anti-CD276 Recombinant Antibody; Fab Fragment (HPAB-M0423-YC-F(E)) | Cyt, FuncS | Mouse Fab |
| HPAB-M0429-YC-F(E) | Human Anti-CD276 Recombinant Antibody; Fab Fragment (HPAB-M0429-YC-F(E)) | Cyt, FuncS | Chimeric (Mouse/Human) Fab |
| HPAB-M0430-YC-F(E) | Human Anti-CD276 Recombinant Antibody; Fab Fragment (HPAB-M0430-YC-F(E)) | Cyt, FuncS | Chimeric (Mouse/Human) Fab |
| HPAB-M0431-YC-F(E) | Human Anti-CD276 Recombinant Antibody; Fab Fragment (HPAB-M0431-YC-F(E)) | Cyt, FuncS | Chimeric (Mouse/Human) Fab |
ADCC Enhanced Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| AFC-TAB-424CQ | Afuco™ Anti-CD276 ADCC Recombinant Antibody (Enoblituzumab), ADCC Enhanced | ELISA, IHC, FC, IP, IF, FuncS | ADCC enhanced antibody |
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For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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