Anti-CD28 antibody is a Mouse antibody of IgG class that binds to an CD28.
Figure 1 Activation of T cell hybridoma cells via superagonistic CD28-specific mAb.
The 58 T cell hybridoma line supplemented with a rat TCR was retrovirally transduced with mouse CD28 containing the following replacements: (A) the whole extracellular part of rat CD28, (B) the rat C′′D loop, or (C) the human C′′D loop. The cell lines were left unstimulated or were stimulated via immobilized anti-TCR mAb R73, superagonistic CD28-specific mAb (JJ316 for rat, 5.11A1 for human) at the indicated final concentrations, ranging from 10 to 1 μg/ml, or conventional CD28-specific mAb (JJ319 for rat, 37.51 for the chimeric molecules). After 2 d, supernatants were tested for the presence of mouse IL-2 by ELISA. Because stimulation with conventional CD28-specific mAb did not result in substantial IL-2 production at any concentration tested, only results using the highest concentration (10 μg/ml) are presented. One of at least three independent experiments with similar results is shown.
Lühder, F., Huang, Y., Dennehy, K. M., Guntermann, C., Müller, I., Winkler, E., ... & Hünig, T. (2003). Topological requirements and signaling properties of T cell–activating, anti-CD28 antibody superagonists. Journal of Experimental Medicine, 197(8), 955-966.
Figure 2 Activation markers, proliferation and cytokine release from human T cells in response to dry-coated anti-CD28 dAb-002.
Human T cells were added to wells coated with varying concentrations of Vκ dummy dAb, dAb-002, or the superagonist Ab mAb 5.11A1 and cell activation determined by increased expression of the activation Ags CD69 (A) cytokine release. The concentration of dAb or mAb 5.11A1 added to each well for dry-coating is indicated in (A). Data are mean ± SD of 10 different blood donors.
Suchard, S. J., Davis, P. M., Kansal, S., Stetsko, D. K., Brosius, R., Tamura, J., ... & Yang, Z. (2013). A monovalent anti-human CD28 domain antibody antagonist: preclinical efficacy and safety. The Journal of Immunology, 191(9), 4599-4610.
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CAT | Product Name | Application | Type |
---|---|---|---|
TAB-H46 | Humanized Anti-CD28 Recombinant Antibody (clone Lulizumab) | IP, IF, FuncS, FC, Neut, ELISA, IHC | Humanized V-kappa |
TAB-064LC-F(E) | Anti-Human CD28 Recombinant Antibody Fab Fragment (TGN1412) | FC | Humanized antibody |
TAB-065LC-F(E) | Human Anti-CD28 Recombinant Antibody; Fab Fragment (TAB-065LC-F(E)) | ELISA | Humanized Fab |
TAB-066LC-F(E) | Human Anti-CD28 Recombinant Antibody; Fab Fragment (TAB-066LC-F(E)) | ELISA | Humanized Fab |
TAB-069LC-F(E) | Anti-Human CD28 Recombinant Antibody Fab Fragment (HuTN228) | FC, ELISA | Humanized antibody |
CAT | Product Name | Application | Type |
---|---|---|---|
PSBL-365 | Mouse Anti-CD28 Recombinant Antibody (clone 1C6); scFv Fragment | WB, ELISA, FuncS | Mouse scFv |
FAMAB-0013-CN-S(P) | Mouse Anti-CD28 Recombinant Antibody (clone AV7); scFv Fragment | FC | Mouse scFv |
HPAB-0245-FY-S(P) | Mouse Anti-CD28 Recombinant Antibody; scFv Fragment (HPAB-0245-FY-S(P)) | WB, IP, IF, FC | Mouse scFv |
HPAB-1489-FY-F(E) | Human Anti-CD28 Recombinant Antibody (clone 2E12); scFv Fragment | WB, ELISA | Human scFv |
HPAB-1144WJ-S(P) | Human Anti-CD28 Recombinant Antibody; scFv Fragment (HPAB-1144WJ-S(P)) | ELISA | Humanized scFv |
CAT | Product Name | Application | Type |
---|---|---|---|
PFBL-365 | Mouse Anti-CD28 Recombinant Antibody (clone 1C6); Fab Fragment | WB, ELISA, FuncS | Mouse Fab |
FAMAB-0013-CN-F(E) | Mouse Anti-CD28 Recombinant Antibody (clone AV7); Fab Fragment | FC | Mouse Fab |
HPAB-0132CQ-F(E) | Human Anti-CD28 Recombinant Antibody (clone FR255734); Fab Fragment | ELISA, IHC, Block | Human Fab |
HPAB-0245-FY-F(E) | Mouse Anti-CD28 Recombinant Antibody; Fab Fragment (HPAB-0245-FY-F(E)) | WB, IP, IF, FC | Mouse Fab |
HPAB-1589WJ-F(E) | Mouse Anti-CD28 Recombinant Antibody; Fab Fragment (clone 2E12) | ELISA, WB | Mouse Fab |
CAT | Product Name | Application | Type |
---|---|---|---|
TAB-068LC | Anti-Human CD28 Recombinant Antibody (ChTN228) | ELISA, FC | Chimeric antibody (mouse/human) |
TAB-068LC-S(P) | Anti-Human CD28 Recombinant Antibody scFv Fragment (ChTN228) | FC, ELISA | Chimeric antibody (mouse/human) |
TAB-068LC-F(E) | Anti-Human CD28 Recombinant Antibody Fab Fragment (ChTN228) | FC, ELISA | Chimeric antibody (mouse/human) |
CAT | Product Name | Application | Type |
---|---|---|---|
PABX-031-S (P) | Recombinant Mouse Anti-CD28 Antibody scFv Fragment (5.11A1) | WB, ELISA, FuncS | scFv |
CAT | Product Name | Application | Type |
---|---|---|---|
PABX-031-F (E) | Recombinant Mouse Anti-CD28 Antibody Fab Fragment (5.11A1) | WB, ELISA, FuncS | Fab |
CAT | Product Name | Application | Type |
---|---|---|---|
NEUT-334CQ | Mouse Anti-CD28 Recombinant Antibody (clone CD28.6) | FC, FuncS, Neut, WB | Mouse IgG2a, κ |
NEUT-337CQ | Hamster Anti-CD28 Recombinant Antibody (clone MAB0818) | Neut | Hamster IgG1 |
NEUT-338CQ | Hamster Anti-CD28 Recombinant Antibody (clone PV-1) | ELISA, FC, Neut | Hamster IgG |
CAT | Product Name | Application | Type |
---|---|---|---|
NEUT-335CQ | Hamster Anti-CD28 Recombinant Antibody (clone 37.51) | IHC-Fr, FC, IP, Block, FuncS | Hamster IgG |
NEUT-336CQ | Mouse Anti-CD28 Recombinant Antibody (clone E18) | FC, Costim, Block | Mouse IgG2b, κ |
CAT | Product Name | Application | Type |
---|---|---|---|
MOR-4053 | Hi-Affi™ Rabbit Anti-CD28 Recombinant Antibody (clone SI120DS) | FC | Rabbit IgG |
MOR-4054 | Hi-Affi™ Rabbit Anti-CD28 Recombinant Antibody (clone SI121DS) | ELISA, FC | Rabbit IgG |
CAT | Product Name | Application | Type |
---|---|---|---|
FAMAB-0320YC-VH | Recombinant Humanized Anti-CD28 Single Domain Antibody (re-CD28 clone 1) | ELISA | Humanized VH |
FAMAB-0321YC-VH | Recombinant Humanized Anti-CD28 Single Domain Antibody (re-CD28 clone 2) | ELISA | Humanized VH |
CAT | Product Name | Application | Type |
---|---|---|---|
FAMAB-0013-CN | Mouse Anti-CD28 Recombinant Antibody (clone AV7) | FC | Mouse IgG |
HPAB-0245-FY | Mouse Anti-CD28 Recombinant Antibody (HPAB-0245-FY) | WB, IP, IF, FC | Mouse IgG1 |
HPAB-0246-FY | Mouse Anti-CD28 Recombinant Antibody (HPAB-0246-FY) | FC, FuncS, ICC, IHC, IP, WB | Mouse IgG1 |
FN-227CQ | Mouse Anti-CD28 Recombinant Antibody (clone JJ319) | FC, FuncS | Mouse IgG1 |
HPAB-1144WJ | Human Anti-CD28 Recombinant Antibody (HPAB-1144WJ) | ELISA | Humanized IgG |
CAT | Product Name | Application | Type |
---|---|---|---|
AFC-TAB-H47 | Afuco™ Anti-CD28 ADCC Recombinant Antibody (Lumretuzumab), ADCC Enhanced | ELISA, FC, IP, FuncS, IF, Neut | ADCC enhanced antibody |
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