Recombinant Mouse Anti-CD28 Antibody (5.11A1) (CAT#: PABX-031)

Anti-CD28 antibody is a Mouse antibody of IgG class that binds to an CD28.

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Figure 1 Activation of T cell hybridoma cells via superagonistic CD28-specific mAb.

Figure 1 Activation of T cell hybridoma cells via superagonistic CD28-specific mAb.

The 58 T cell hybridoma line supplemented with a rat TCR was retrovirally transduced with mouse CD28 containing the following replacements: (A) the whole extracellular part of rat CD28, (B) the rat C′′D loop, or (C) the human C′′D loop. The cell lines were left unstimulated or were stimulated via immobilized anti-TCR mAb R73, superagonistic CD28-specific mAb (JJ316 for rat, 5.11A1 for human) at the indicated final concentrations, ranging from 10 to 1 μg/ml, or conventional CD28-specific mAb (JJ319 for rat, 37.51 for the chimeric molecules). After 2 d, supernatants were tested for the presence of mouse IL-2 by ELISA. Because stimulation with conventional CD28-specific mAb did not result in substantial IL-2 production at any concentration tested, only results using the highest concentration (10 μg/ml) are presented. One of at least three independent experiments with similar results is shown.

Lühder, F., Huang, Y., Dennehy, K. M., Guntermann, C., Müller, I., Winkler, E., ... & Hünig, T. (2003). Topological requirements and signaling properties of T cell–activating, anti-CD28 antibody superagonists. Journal of Experimental Medicine, 197(8), 955-966.


Figure 2 Activation markers, proliferation and cytokine release from human T cells in response to dry-coated anti-CD28 dAb-002.

Figure 2 Activation markers, proliferation and cytokine release from human T cells in response to dry-coated anti-CD28 dAb-002.

Human T cells were added to wells coated with varying concentrations of Vκ dummy dAb, dAb-002, or the superagonist Ab mAb 5.11A1 and cell activation determined by increased expression of the activation Ags CD69 (A) cytokine release. The concentration of dAb or mAb 5.11A1 added to each well for dry-coating is indicated in (A). Data are mean ± SD of 10 different blood donors.

Suchard, S. J., Davis, P. M., Kansal, S., Stetsko, D. K., Brosius, R., Tamura, J., ... & Yang, Z. (2013). A monovalent anti-human CD28 domain antibody antagonist: preclinical efficacy and safety. The Journal of Immunology, 191(9), 4599-4610.


  • Immunogen
  • The details of the immunogen for this antibody are not available.
  • Host Species
  • Mouse
  • Derivation
  • Mouse
  • Type
  • IgG
  • Specificity
  • Tested positive against native Human CD28
  • Species Reactivity
  • Human
  • Clone
  • 5. 11A1
  • Applications
  • WB, ELISA, FuncS, Activ

Product Property

  • Purity
  • >95% by SDS-PAGE and HPLC analysis
  • Storage
  • Store the antibody (in aliquots) at -20°C. Avoid repeated freezing and thawing of samples.


  • Application Notes
  • The CD28 antibody has been reported in applications of Western Blot, Enzyme-linked Immunosorbent Assay, Functional Assay, Activation.


  • Alternative Names
  • CD28; CD28 molecule; Tp44; T-cell-specific surface glycoprotein CD28; CD28 antigen;

Related Resources

  • Related Diseases

Product Notes

This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production

See more details about Hi-Affi™ recombinant antibody benefits.


Download resources about recombinant antibody development and antibody engineering to boost your research.

See other products for "Clone 5. 11A1"

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Chimeric Antibody

CAT Product Name Application Type
TAB-068LC Anti-Human CD28 Recombinant Antibody (ChTN228) ELISA, FC Chimeric antibody (mouse/human)
TAB-068LC-S(P) Anti-Human CD28 Recombinant Antibody scFv Fragment (ChTN228) FC, ELISA Chimeric antibody (mouse/human)
TAB-068LC-F(E) Anti-Human CD28 Recombinant Antibody Fab Fragment (ChTN228) FC, ELISA Chimeric antibody (mouse/human)

Human Antibody

CAT Product Name Application Type
PABX-031-S (P) Recombinant Mouse Anti-CD28 Antibody scFv Fragment (5.11A1) WB, ELISA, FuncS scFv

Mouse Antibody

CAT Product Name Application Type
PABX-031-F (E) Recombinant Mouse Anti-CD28 Antibody Fab Fragment (5.11A1) WB, ELISA, FuncS Fab

Neutralizing Antibody

Blocking Antibody

CAT Product Name Application Type
NEUT-335CQ Hamster Anti-CD28 Recombinant Antibody (clone 37.51) IHC-Fr, FC, IP, Block, FuncS Hamster IgG
NEUT-336CQ Mouse Anti-CD28 Recombinant Antibody (clone E18) FC, Costim, Block Mouse IgG2b, κ

Rabbit Monoclonal Antibody

ADCC Enhanced Antibody

CAT Product Name Application Type
AFC-TAB-H47 Afuco™ Anti-CD28 ADCC Recombinant Antibody (Lumretuzumab), ADCC Enhanced ELISA, FC, IP, FuncS, IF, Neut ADCC enhanced antibody

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For Research Use Only. Not For Clinical Use.

For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.

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