Anti-Human CD28 Recombinant Antibody (TGN1412) (CAT#: TAB-064LC)
TAB-064LC is a superagonistic monoclonal antibody (mAb) capable of fully activating T-cells by cross-linking CD28 molecules, even without signal 1, which sets it apart from conventional anti-CD28 mAbs. TAB-064LC exhibits a binding affinity to human CD28, with an EC50 value of 4.4 nM. TAB-064LC exhibits a binding affinity to human T cells, with EC50 values measured at 2.5 nM.
Specific Inquiry
ELISA
Figure 1 Anti-CD28 Antibody (TAB-064LC) in ELISA
ELISA analysis of TAB-064LC was performed by coating with Recombinant Human CD28 Protein (11524-HCCH). Then blocked with BSA, and incubated with Anti-Human CD28 Antibody (TAB-064LC). The HRP-conjugated goat anti-human IgG as a secondary antibody. Detection was performed using TMB substrate and stopped with sulfuric acid. The absorbances were read on a spectrophotometer at 450 nm.
WB
Figure 2 Anti-CD28 Antibody (TAB-064LC) in WB
Western blot analysis of TAB-064LC was performed by loading Recombinant Human CD28 Protein (11524-HCCH) onto a 12% Tris-HCl polyacrylamide gel.
Lane 1: Non-Reducing Antigen (0.5 μg)
Lane 2: Reducing Antigen (0.5 μg)
SDS-PAGE
Figure 3 Anti-Human CD28 Recombinant Antibody (TAB-064LC), Fc Silenced, in SDS-PAGE
SDS-PAGE analysis of TAB-064LC, Fc silenced, in non-reduced (Lane 1) and reduced (Lane 2) conditions. Gel stained for 30 minutes with Coomassie Blue. As a result, different β-mercaptoethanol-reduced proteins (Heavy chain and Light chain) migrate as about 50 kDa and 25 kDa, respectively.
The purity of TAB-064LC-FcS was greater than 95% as determined by SDS-PAGE.
SEC-HPLC
Figure 4 Anti-Human CD28 Recombinant Antibody (TAB-064LC), Fc Silenced, in HPLC
The purity of TAB-064LC, Fc silenced, was greater than 95% as determined by SEC-HPLC.
WB
Figure 5 Anti-Human CD28 Recombinant Antibody (TAB-064LC), Fc Silenced, in WB
Western blot analysis of TAB-064LC, Fc silenced, was performed by loading recombinant human CD28 protein.
TAB-064LC-FcS incubation concentration: 2 ng/μL.
The secondary antibody: HRP-goat anti-human IgG
Lane 1: Reducing Antigen (0.1 μg)
Lane 2: Reducing Antigen (0.3 μg)
Lane 3: Reducing Antigen (0.6 μg)
ELISA
Figure 6 Anti-Human CD28 Recombinant Antibody (TAB-064LC), Fc Silenced, in ELISA
ELISA analysis of TAB-064LC, Fc silenced, was performed by coating with recombinant human CD28 protein. Then blocked with BSA, and incubated with Anti-Human CD28 Antibody (TGN1412), Fc Silenced. The Goat Anti-Human IgG-HRP as a secondary antibody. Detection was performed using TMB substrate and stopped with sulfuric acid. The absorbances were read on a spectrophotometer at 450 nm.
Specifications
- Host Species
- Human
- Derivation
- Humanized
- Type
- Humanized antibody
- Specificity
- Human
- Clone
- TGN1412
- Applications
- FC, Stim
- Related Disease
- Inflammatory diseases, Autoimmune diseases
Applications
- Application Notes
- This antibody has been tested for use in Stimulation. Please see the Published Data for further information.
Target
Related Resources
Rheumatoid Arthritis
Systemic Lupus Erythematosus
Allograft Rejection
Autoimmune Thyroid Disease
Graft-versus-Host Disease
Maturity Onset Diabetes of the Young
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
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| CAT | Product Name | Application | Type |
|---|---|---|---|
| PFBL-365 | Mouse Anti-CD28 Recombinant Antibody (clone 1C6); Fab Fragment | WB, ELISA, FuncS | Mouse Fab |
| FAMAB-0013-CN-F(E) | Mouse Anti-CD28 Recombinant Antibody (clone AV7); Fab Fragment | FC | Mouse Fab |
| HPAB-1489-FY-S(P) | Human Anti-CD28 Recombinant Antibody (clone 2E12); Fab Fragment | WB, ELISA | Human Fab |
| HPAB-1144WJ-F(E) | Human Anti-CD28 Recombinant Antibody; Fab Fragment (HPAB-1144WJ-F(E)) | ELISA | Humanized Fab |
| HPAB-1589WJ-F(E) | Mouse Anti-CD28 Recombinant Antibody; Fab Fragment (clone 2E12) | ELISA, WB | Mouse Fab |
Customer Reviews and Q&As
Customer Review
Q&As
-
Great for CD28 Studies
The Anti-CD28 Therapeutic Antibody has been excellent for our CD28 studies. Its specificity and sensitivity are top-notch, providing clear and reliable results. This antibody has significantly improved the accuracy of our experiments and saved us a lot of troubleshooting time. -
Effective in Immunoassays
We incorporated this antibody into our immunoassays, and it has been a great asset. The specificity for CD28 is precise, allowing for accurate detection and analysis. The reliable performance has made it an invaluable tool in our lab. -
Consistent and Reliable
This antibody has proven to be consistent and reliable in our assays. The high-quality, reproducible results have significantly improved our experiments, making it a crucial part of our research toolkit. The strong affinity and specificity for CD28 have been particularly beneficial.
Cite This Product
To accurately reference this product in your publication, please use the following citation information:
(Creative Biolabs Cat# TAB-064LC, RRID: AB_3111785)
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For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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