Anti-Human CD28 Recombinant Antibody (TGN1412) (CAT#: TAB-064LC)

TAB-064LC is a superagonistic monoclonal antibody (mAb) capable of fully activating T-cells by cross-linking CD28 molecules, even without signal 1, which sets it apart from conventional anti-CD28 mAbs. TAB-064LC exhibits a binding affinity to human CD28, with an EC50 value of 4.4 nM. TAB-064LC exhibits a binding affinity to human T cells, with EC50 values measured at 2.5 nM.


Specific Inquiry
  • Size:
  • Conjugation:
  • Endotoxin:
  • Purity:
  • Fc Engineering:
  • Tested Data
  • Gene Expression
  • Datasheet
  • MSDS
  • COA
ELISA

Figure 1 Anti-CD28 Antibody (TAB-064LC) in ELISA

Figure 1 Anti-CD28 Antibody (TAB-064LC) in ELISA

ELISA analysis of TAB-064LC was performed by coating with Recombinant Human CD28 Protein (11524-HCCH). Then blocked with BSA, and incubated with Anti-Human CD28 Antibody (TAB-064LC). The HRP-conjugated goat anti-human IgG as a secondary antibody. Detection was performed using TMB substrate and stopped with sulfuric acid. The absorbances were read on a spectrophotometer at 450 nm.

WB

Figure 2 Anti-CD28 Antibody (TAB-064LC) in WB

Figure 2 Anti-CD28 Antibody (TAB-064LC) in WB

Western blot analysis of TAB-064LC was performed by loading Recombinant Human CD28 Protein (11524-HCCH) onto a 12% Tris-HCl polyacrylamide gel.
Lane 1: Non-Reducing Antigen (0.5 μg)
Lane 2: Reducing Antigen (0.5 μg)

SDS-PAGE

Figure 3 Anti-Human CD28 Recombinant Antibody (TAB-064LC), Fc Silenced, in SDS-PAGE

Figure 3 Anti-Human CD28 Recombinant Antibody (TAB-064LC), Fc Silenced, in SDS-PAGE

SDS-PAGE analysis of TAB-064LC, Fc silenced, in non-reduced (Lane 1) and reduced (Lane 2) conditions. Gel stained for 30 minutes with Coomassie Blue. As a result, different β-mercaptoethanol-reduced proteins (Heavy chain and Light chain) migrate as about 50 kDa and 25 kDa, respectively.

The purity of TAB-064LC-FcS was greater than 95% as determined by SDS-PAGE.

SEC-HPLC

Figure 4 Anti-Human CD28 Recombinant Antibody (TAB-064LC), Fc Silenced, in HPLC

Figure 4 Anti-Human CD28 Recombinant Antibody (TAB-064LC), Fc Silenced, in HPLC

The purity of TAB-064LC, Fc silenced, was greater than 95% as determined by SEC-HPLC.

WB

Figure 5 Anti-Human CD28 Recombinant Antibody (TAB-064LC), Fc Silenced, in WB

Figure 5 Anti-Human CD28 Recombinant Antibody (TAB-064LC), Fc Silenced, in WB

Western blot analysis of TAB-064LC, Fc silenced, was performed by loading recombinant human CD28 protein.

TAB-064LC-FcS incubation concentration: 2 ng/μL.
The secondary antibody: HRP-goat anti-human IgG
Lane 1: Reducing Antigen (0.1 μg)
Lane 2: Reducing Antigen (0.3 μg)
Lane 3: Reducing Antigen (0.6 μg)

ELISA

Figure 6 Anti-Human CD28 Recombinant Antibody (TAB-064LC), Fc Silenced, in ELISA

Figure 6 Anti-Human CD28 Recombinant Antibody (TAB-064LC), Fc Silenced, in ELISA

ELISA analysis of TAB-064LC, Fc silenced, was performed by coating with recombinant human CD28 protein. Then blocked with BSA, and incubated with Anti-Human CD28 Antibody (TGN1412), Fc Silenced. The Goat Anti-Human IgG-HRP as a secondary antibody. Detection was performed using TMB substrate and stopped with sulfuric acid. The absorbances were read on a spectrophotometer at 450 nm.


Specifications

  • Host Species
  • Human
  • Derivation
  • Humanized
  • Type
  • Humanized antibody
  • Specificity
  • Human
  • Clone
  • TGN1412
  • Applications
  • FC, Stim
  • Related Disease
  • Inflammatory diseases, Autoimmune diseases

Applications

  • Application Notes
  • This antibody has been tested for use in Stimulation. Please see the Published Data for further information.

Target

  • Alternative Names
  • Tp44; CD28

Related Resources

  • Related Diseases

Product Notes

This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production

See more details about Hi-Affi™ recombinant antibody benefits.

Downloads

Download resources about recombinant antibody development and antibody engineering to boost your research.

See other products for "Clone TGN1412"

See other products for "CD28"

Chimeric Antibody

CAT Product Name Application Type
TAB-068LC Anti-Human CD28 Recombinant Antibody (ChTN228) ELISA, FC Chimeric antibody (mouse/human)
TAB-068LC-S(P) Anti-Human CD28 Recombinant Antibody scFv Fragment (ChTN228) FC, ELISA Chimeric antibody (mouse/human)
TAB-068LC-F(E) Anti-Human CD28 Recombinant Antibody Fab Fragment (ChTN228) FC, ELISA Chimeric antibody (mouse/human)

Mouse Antibody

CAT Product Name Application Type
PABX-031 Recombinant Mouse Anti-CD28 Antibody (5.11A1) WB, ELISA, FuncS, Activ IgG
PABX-031-F (E) Recombinant Mouse Anti-CD28 Antibody Fab Fragment (5.11A1) WB, ELISA, FuncS Fab

Human Antibody

CAT Product Name Application Type
PABX-031-S (P) Recombinant Mouse Anti-CD28 Antibody scFv Fragment (5.11A1) WB, ELISA, FuncS scFv

Neutralizing Antibody

Blocking Antibody

CAT Product Name Application Type
NEUT-335CQ Hamster Anti-CD28 Recombinant Antibody (clone 37.51) IHC-Fr, FC, IP, Block, FuncS Hamster IgG
NEUT-336CQ Mouse Anti-CD28 Recombinant Antibody (clone E18) FC, Costim, Block Mouse IgG2b, κ

Rabbit Monoclonal Antibody

Recombinant Antibody

ADCC Enhanced Antibody

CAT Product Name Application Type
AFC-TAB-H47 Afuco™ Anti-CD28 ADCC Recombinant Antibody (Lumretuzumab), ADCC Enhanced ELISA, FC, IP, FuncS, IF, Neut ADCC enhanced antibody

Customer Reviews and Q&As

Submit a review or a question
There are currently no Customer reviews or questions for TAB-064LC. Click the button above to contact us or submit your feedback about this product.
View the frequently asked questions answered by Creative Biolabs Support.

For Research Use Only. Not For Clinical Use.

For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.

Send Inquiry

This site is protected by reCAPTCHA and the Google Privacy Policy and Terms of Service apply.

© 2024 Creative Biolabs.
  • 0
  • 0
Cart

    Go to compare