What is the main purpose of M195 (IgG)-PE, the anti-CD33 immunotoxin?

The anti-CD33 immunotoxin M195 (IgG)-PE targets CD33 markers on leukemic cells. By binding to these markers, the immunotoxin facilitates the destruction of leukemic cells through a cytotoxic payload. Specifically, the Pseudomonas exotoxin portion of the immunotoxin interferes with the protein synthesis of the targeted cells, leading to their apoptosis.

In comparison to other immunotoxins, how is anti-CD33 immunotoxin M195 (IgG)-PE different?

Anti-CD33 immunotoxin M195 (IgG)-PE is distinct in its specific targeting of the CD33 antigen on leukemic cells. It combines the targeting ability of an anti-CD33 antibody with the cytotoxic action of Pseudomonas exotoxin. Its unique mechanism reduces off-target effects and enhances specificity compared to other broader immunotoxins.

What role does anti-CD33 immunotoxin M195 (IgG)-PE play in the larger immunotherapy context?

Anti-CD33 immunotoxin M195 (IgG)-PE is part of the growing field of immunotherapy, which leverages the body's immune system to fight cancer. Unlike traditional immunotherapies that broadly modulate immune responses, this treatment combines a targeted antibody with a cytotoxic agent, offering a precise and potent approach to eradicate cancer cells.

anti-CD33 immunotoxin M195 (IgG)-PE

CAT#: AGTO-L050E

This immunotoxin was achieved by conjugating/fusing the Anti-CD33 monoclonal antibody to Pseudomonas exotoxin. It was expressed in CHO and purified with affinity chromatography. The immunotoxin selectively binds to leukemia cells and enters cells by receptor-mediated endocytosis, then PE cleaved fragments the size of Mr 37000 and 27000, the large fragment and finally into the Golgi and elongation factor 2 (EM) combining it glycosylation inactivation, inhibition of protein synthesis, leading to apoptosis. It was designed for treatment of leukemia.

Gene Expression

Subcellular Location and Protein Expression Figure 1 IF staining of human cell line PC-3

Immunofluorescent staining of human cell line PC-3 shows localization to nucleoplasm & plasma membrane.

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/64807/1186_A4_3_selected.jpg

Subcellular Location and Protein Expression Figure 2 IHC staining of human lymph node

Immunohistochemical staining of human lymph node shows membranous and cytoplasmic positivity in germinal center cells and non-germinal center cells.

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/35832/113039_A_9_8_selected.jpg

Subcellular Location and Protein Expression Figure 3 IHC staining of human tonsil

Immunohistochemical staining of human tonsil shows cytoplasmic positivity in germinal center cells and non-germinal center cells.

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/11442/26430_A_6_8_selected.jpg

Subcellular Location and Protein Expression Figure 4 IF staining of human cell line RH-30

Immunofluorescent staining of human cell line RH-30 shows localization to nucleoplasm.

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/64807/1413_G6_1_red_green.jpg

Subcellular Location and Protein Expression Figure 5 IF staining of human cell line U-2 OS

Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm.

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/64807/1200_A4_2_red_green.jpg

Normal Tissue Figure 6 Kidney

Cells in tubules
Staining:Medium
Intensity: Strong
Quantity: <25%
Location: Cytoplasmic/membranous

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/79996/170702_A_9_5.jpg

Normal Tissue Figure 7 Lymph node

Non-germinal center cells
Staining:Medium
Intensity: Strong
Quantity: <25%
Location: Cytoplasmic/membranous

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/79996/170702_A_9_8.jpg

Normal Tissue Figure 8 Spleen

Cells in red pulp
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/79996/170702_B_8_4.jpg

RNA Expression Figure 9 RNA cell line category: Cell line enhanced (HEL, HL-60, HMC-1, NB-4, RPMI-8226, THP-1, U-937)

Cell lines ordered by descending RNA expression order.

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000105383-CD33

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Specifications

Host Species
Mouse

Species Reactivity
Human

Clone
M195

Molecule Class
L kappa; IgG1-PE

Toxin Description
Pseudomonas aeruginosa exotoxin (Pseudomonas Aeruginosa Exotoxin A, PE) is a cell toxin produced by P. aeruginosa. Divided into three domains in space. DomainIII (s 405~613 amino acids) having ADP ribosylation activity, can inhibit protein synthesis, PE on toxicity mechanisms of cell depends on its Domainlll. After PE molecules into mammals, Domain Ia and widely distributed in mammalian cells α 2 macroglobulin receptor binding was endocytic, then PE cleaved fragments the size of Mr 37000 and 27000, the large fragment and finally into the Golgi and elongation factor 2 (EM) combining it glycosylation inactivation, inhibition of protein synthesis, leading to apoptosis.

Applications
This product is suitable for use in cytotoxicity assay, functional assay and immunotherapy.

Related Disease
Leukemia

Target

Alternative Names
CD33; CD33 molecule; p67; SIGLEC3; SIGLEC-3; myeloid cell surface antigen CD33; gp67; CD33 antigen (gp67); sialic acid binding Ig-like lectin 3; sialic acid-binding Ig-like lectin 3; Leukemias; Pseudomonas exotoxin; Pseudomonas exotoxin A; Pseudomonas aeruginosa exotoxin; Pseudomonas aeruginosa exotoxin A; PE; PE38; PE40; ETA; PE33; PE35

Gene ID
945

UniProt ID
P20138

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Breast cancer biomarkers at key points during disease progression

Optimistic results

The results from anti-CD33 immunotoxin M195 (IgG)-PE have been very optimistic.

Less invasive

Anti-CD33 immunotoxin M195 (IgG)-PE is less invasive than traditional chemotherapy.

Highly recommended

I highly recommend anti-CD33 immunotoxin M195 (IgG)-PE for anyone studying AML.

Q&As

What is the main purpose of M195 (IgG)-PE, the anti-CD33 immunotoxin?

A: The anti-CD33 immunotoxin M195 (IgG)-PE targets CD33 markers on leukemic cells. By binding to these markers, the immunotoxin facilitates the destruction of leukemic cells through a cytotoxic payload. Specifically, the Pseudomonas exotoxin portion of the immunotoxin interferes with the protein synthesis of the targeted cells, leading to their apoptosis.
In comparison to other immunotoxins, how is anti-CD33 immunotoxin M195 (IgG)-PE different?

A: Anti-CD33 immunotoxin M195 (IgG)-PE is distinct in its specific targeting of the CD33 antigen on leukemic cells. It combines the targeting ability of an anti-CD33 antibody with the cytotoxic action of Pseudomonas exotoxin. Its unique mechanism reduces off-target effects and enhances specificity compared to other broader immunotoxins.
What role does anti-CD33 immunotoxin M195 (IgG)-PE play in the larger immunotherapy context?

A: Anti-CD33 immunotoxin M195 (IgG)-PE is part of the growing field of immunotherapy, which leverages the body's immune system to fight cancer. Unlike traditional immunotherapies that broadly modulate immune responses, this treatment combines a targeted antibody with a cytotoxic agent, offering a precise and potent approach to eradicate cancer cells.

View the frequently asked questions answered by Creative Biolabs Support.

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Product Notes

This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production

See more details about Hi-Affi™ recombinant antibody benefits.

Datasheet

Datasheet - AGTO-L050E.pdf

MSDS

MSDS - AGTO-L050E.pdf

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