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    anti-CD33 immunotoxin M195 (IgG)-PE (CAT#: AGTO-L050E)

    This immunotoxin was achieved by conjugating/fusing the Anti-CD33 monoclonal antibody to Pseudomonas exotoxin. It was expressed in CHO and purified with affinity chromatography. The immunotoxin selectively binds to leukemia cells and enters cells by receptor-mediated endocytosis, then PE cleaved fragments the size of Mr 37000 and 27000, the large fragment and finally into the Golgi and elongation factor 2 (EM) combining it glycosylation inactivation, inhibition of protein synthesis, leading to apoptosis. It was designed for treatment of leukemia.

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    • Gene Expression
    • Datasheet
    • MSDS
    • COA
    Subcellular Location and Protein Expression
    Figure 1 IF staining of human cell line PC-3
    Figure 1 IF staining of human cell line PC-3
    (Immunofluorescent staining of human cell line PC-3 shows localization to nucleoplasm & plasma membrane.)
    * Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/64807/1186_A4_3_selected.jpg
    Figure 2 IHC staining of human lymph node
    Figure 2 IHC staining of human lymph node
    (Immunohistochemical staining of human lymph node shows membranous and cytoplasmic positivity in germinal center cells and non-germinal center cells.)
    * Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/35832/113039_A_9_8_selected.jpg
    Figure 3 IHC staining of human tonsil
    Figure 3 IHC staining of human tonsil
    (Immunohistochemical staining of human tonsil shows cytoplasmic positivity in germinal center cells and non-germinal center cells.)
    * Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/11442/26430_A_6_8_selected.jpg
    Figure 4 IF staining of human cell line RH-30
    Figure 4 IF staining of human cell line RH-30
    (Immunofluorescent staining of human cell line RH-30 shows localization to nucleoplasm.)
    * Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/64807/1413_G6_1_red_green.jpg
    Figure 5 IF staining of human cell line U-2 OS
    Figure 5 IF staining of human cell line U-2 OS
    (Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm.)
    * Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/64807/1200_A4_2_red_green.jpg
    Normal Tissue
    Figure 6 Kidney
    Figure 6 Kidney
    (Cells in tubules
    Staining:Medium
    Intensity: Strong
    Quantity: <25%
    Location: Cytoplasmic/membranous)
    * Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/79996/170702_A_9_5.jpg
    Figure 7 Lymph node
    Figure 7 Lymph node
    (Non-germinal center cells
    Staining:Medium
    Intensity: Strong
    Quantity: <25%
    Location: Cytoplasmic/membranous)
    * Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/79996/170702_A_9_8.jpg
    Figure 8 Spleen
    Figure 8 Spleen
    (Cells in red pulp
    Staining:Medium
    Intensity: Moderate
    Quantity:>75%
    Location: Cytoplasmic/membranous)
    * Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/79996/170702_B_8_4.jpg
    RNA Expression
    Figure 9 RNA cell line category: Cell line enhanced (HEL, HL-60, HMC-1, NB-4, RPMI-8226, THP-1, U-937)
    Figure 9 RNA cell line category: Cell line enhanced (HEL, HL-60, HMC-1, NB-4, RPMI-8226, THP-1, U-937)
    (Cell lines ordered by descending RNA expression order.)
    * Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000105383-CD33

    Specifications

    • Host Species
    • Mouse
    • Species Reactivity
    • Human
    • Clone
    • M195
    • Molecule Class
    • L kappa; IgG1-PE
    • Toxin Description
    • Pseudomonas aeruginosa exotoxin (Pseudomonas Aeruginosa Exotoxin A, PE) is a cell toxin produced by P. aeruginosa. Divided into three domains in space. DomainIII (s 405~613 amino acids) having ADP ribosylation activity, can inhibit protein synthesis, PE on toxicity mechanisms of cell depends on its Domainlll. After PE molecules into mammals, Domain Ia and widely distributed in mammalian cells α 2 macroglobulin receptor binding was endocytic, then PE cleaved fragments the size of Mr 37000 and 27000, the large fragment and finally into the Golgi and elongation factor 2 (EM) combining it glycosylation inactivation, inhibition of protein synthesis, leading to apoptosis.
    • Applications
    • This product is suitable for use in cytotoxicity assay, functional assay and immunotherapy.
    • Related Disease
    • Leukemia

    Target

    • Alternative Names
    • CD33; CD33 molecule; p67; SIGLEC3; SIGLEC-3; myeloid cell surface antigen CD33; gp67; CD33 antigen (gp67); sialic acid binding Ig-like lectin 3; sialic acid-binding Ig-like lectin 3; Leukemias; Pseudomonas exotoxin; Pseudomonas exotoxin A; Pseudomonas aeruginosa exotoxin; Pseudomonas aeruginosa exotoxin A; PE; PE38; PE40; ETA; PE33; PE35

    Product Notes

    This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

    • Increased sensitivity
    • Confirmed specificity
    • High repeatability
    • Excellent batch-to-batch consistency
    • Sustainable supply
    • Animal-free production

    See more details about Hi-Affi™ recombinant antibody benefits.

    Downloads

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    Customer Reviews and Q&As

    Customer Review Q&As

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    Optimistic results
    The results from anti-CD33 immunotoxin M195 (IgG)-PE have been very optimistic.

    Less invasive
    Anti-CD33 immunotoxin M195 (IgG)-PE is less invasive than traditional chemotherapy.

    Highly recommended
    I highly recommend anti-CD33 immunotoxin M195 (IgG)-PE for anyone studying AML.

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