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anti-CD33 immunotoxin M195 (IgG)-PE (CAT#: AGTO-L050E)

This immunotoxin was achieved by conjugating/fusing the Anti-CD33 monoclonal antibody to Pseudomonas exotoxin. It was expressed in CHO and purified with affinity chromatography. The immunotoxin selectively binds to leukemia cells and enters cells by receptor-mediated endocytosis, then PE cleaved fragments the size of Mr 37000 and 27000, the large fragment and finally into the Golgi and elongation factor 2 (EM) combining it glycosylation inactivation, inhibition of protein synthesis, leading to apoptosis. It was designed for treatment of leukemia.


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Subcellular Location and Protein Expression
Figure 1 IF staining of human cell line PC-3
Figure 1 IF staining of human cell line PC-3
(Immunofluorescent staining of human cell line PC-3 shows localization to nucleoplasm & plasma membrane.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/64807/1186_A4_3_selected.jpg
Figure 2 IHC staining of human lymph node
Figure 2 IHC staining of human lymph node
(Immunohistochemical staining of human lymph node shows membranous and cytoplasmic positivity in germinal center cells and non-germinal center cells.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/35832/113039_A_9_8_selected.jpg
Figure 3 IHC staining of human tonsil
Figure 3 IHC staining of human tonsil
(Immunohistochemical staining of human tonsil shows cytoplasmic positivity in germinal center cells and non-germinal center cells.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/11442/26430_A_6_8_selected.jpg
Figure 4 IF staining of human cell line RH-30
Figure 4 IF staining of human cell line RH-30
(Immunofluorescent staining of human cell line RH-30 shows localization to nucleoplasm.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/64807/1413_G6_1_red_green.jpg
Figure 5 IF staining of human cell line U-2 OS
Figure 5 IF staining of human cell line U-2 OS
(Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/64807/1200_A4_2_red_green.jpg
Normal Tissue
Figure 6 Kidney
Figure 6 Kidney
(Cells in tubules
Staining:Medium
Intensity: Strong
Quantity: <25%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/79996/170702_A_9_5.jpg
Figure 7 Lymph node
Figure 7 Lymph node
(Non-germinal center cells
Staining:Medium
Intensity: Strong
Quantity: <25%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/79996/170702_A_9_8.jpg
Figure 8 Spleen
Figure 8 Spleen
(Cells in red pulp
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/79996/170702_B_8_4.jpg
RNA Expression
Figure 9 RNA cell line category: Cell line enhanced (HEL, HL-60, HMC-1, NB-4, RPMI-8226, THP-1, U-937)
Figure 9 RNA cell line category: Cell line enhanced (HEL, HL-60, HMC-1, NB-4, RPMI-8226, THP-1, U-937)
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000105383-CD33

Specifications

  • Host Species
  • Mouse
  • Species Reactivity
  • Human
  • Clone
  • M195
  • Molecule Class
  • L kappa; IgG1-PE
  • Toxin Description
  • Pseudomonas aeruginosa exotoxin (Pseudomonas Aeruginosa Exotoxin A, PE) is a cell toxin produced by P. aeruginosa. Divided into three domains in space. DomainIII (s 405~613 amino acids) having ADP ribosylation activity, can inhibit protein synthesis, PE on toxicity mechanisms of cell depends on its Domainlll. After PE molecules into mammals, Domain Ia and widely distributed in mammalian cells α 2 macroglobulin receptor binding was endocytic, then PE cleaved fragments the size of Mr 37000 and 27000, the large fragment and finally into the Golgi and elongation factor 2 (EM) combining it glycosylation inactivation, inhibition of protein synthesis, leading to apoptosis.
  • Applications
  • This product is suitable for use in cytotoxicity assay, functional assay and immunotherapy.
  • Related Disease
  • Leukemia

Target

  • Alternative Names
  • CD33; CD33 molecule; p67; SIGLEC3; SIGLEC-3; myeloid cell surface antigen CD33; gp67; CD33 antigen (gp67); sialic acid binding Ig-like lectin 3; sialic acid-binding Ig-like lectin 3; Leukemias; Pseudomonas exotoxin; Pseudomonas exotoxin A; Pseudomonas aeruginosa exotoxin; Pseudomonas aeruginosa exotoxin A; PE; PE38; PE40; ETA; PE33; PE35

Product Notes

This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production

See more details about Hi-Affi™ recombinant antibody benefits.

Downloads

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Single-domain Antibody

CAT Product Name Application Type
NAB-321-sdAb Recombinant Anti-human CD33 VHH Single Domain Antibody WB, IP, ChiP, Neut, ELISA Llama VHH

Humanized Antibody

CAT Product Name Application Type
TAB-013 Humanized Anti-CD33 Recombinant Antibody (clone Gemtuzumab) IF, IP, Neut, FuncS, ELISA, FC, WB Humanized (from mouse) IgG4, κ
TAB-H37 Anti-Human CD33 Recombinant Antibody (Gemtuzumab Ozogamicin) ELISA, FC, IP, FuncS, IF, Neut, IHC IgG
TAB-756 Anti-CD33 Recombinant Antibody (Lintuzumab) IF, IP, Neut, FuncS, ELISA, FC, ICC IgG1 - kappa
VS3-WK1523 Human Anti-CD33 Recombinant Antibody (clone CDP-771) FC Humanized IgG4

Immunotoxin

CAT Product Name Application Type
AGTO-G008E Anti-CD33 immunotoxin (scFv)-PE Cytotoxicity assay, Function study
AGTO-G008G Anti-CD33 immunotoxin (scFv)-Gel Cytotoxicity assay, Function study
AGTO-L050G anti-CD33 immunotoxin M195 (IgG)-Gel Cytotoxicity assay, Functional assay

Human Antibody

CAT Product Name Application Type
TAB-123LC Anti-Human CD33 Recombinant Antibody FC Human antibody
TAB-146LC Human Anti-CD33 Recombinant Antibody (TAB-146LC) ELISA Human IgG
TAB-123LC-S(P) Anti-Human CD33 Recombinant Antibody scFv Fragment FC Human antibody
TAB-146LC-S(P) Human Anti-CD33 Recombinant Antibody; scFv Fragment (TAB-146LC-S(P)) ELISA Human scFv
TAB-123LC-F(E) Anti-Human CD33 Recombinant Antibody Fab Fragment FC Human antibody

Mouse Antibody

CAT Product Name Application Type
TAB-119LC-S(P) Mouse Anti-CD33 Recombinant Antibody; scFv Fragment (TAB-119LC-S(P)) ELISA, IF, RIA Mouse scFv
TAB-120LC-S(P) Mouse Anti-CD33 Recombinant Antibody; scFv Fragment (TAB-120LC-S(P)) ELISA Mouse scFv
TAB-121LC-S(P) Mouse Anti-CD33 Recombinant Antibody; scFv Fragment (TAB-121LC-S(P)) ELISA Mouse scFv
TAB-122LC-S(P) Mouse Anti-CD33 Recombinant Antibody; scFv Fragment (TAB-122LC-S(P)) ELISA Mouse scFv
TAB-124LC-S(P) Mouse Anti-CD33 Recombinant Antibody; scFv Fragment (TAB-124LC-S(P)) ELISA, FC Mouse scFv

Fab Glycosylation

CAT Product Name Application Type
Gly-013LC Recombinant Anti-Human CD33 Antibody (Fab glycosylation) ELISA Humanized antibody

MHC Tetramer for Cancer

CAT Product Name Application Type
MHC-LC162 APC-A*02:01/Human CD33 (AIISGDSPV) MHC Tetramer FCM
MHC-LC163 PE-A*02:01/Human CD33 A65Y (YIISGDSPV) MHC Tetramer FCM
MHC-LC164 APC-A*02:01/Human CD33 A65Y (YIISGDSPV) MHC Tetramer FCM
MHC-LC2064 PE-A*02:01/Human CD33 (AIISGDSPV) MHC Tetramer FCM
MHC-YF358 A*02:01/Human CD33 65-73 (1Y2L) (YLISGDSPV) MHC Pentamer FCM

Blocking Antibody

CAT Product Name Application Type
NEUT-341CQ Mouse Anti-CD33 Recombinant Antibody (clone WM53) FC, IHC-Fr, ICC, IP, WB, Block Mouse IgG1, κ

Rabbit Monoclonal Antibody

CAT Product Name Application Type
MOR-0553 Hi-Affi™ Rabbit Anti-CD33 Recombinant Antibody (clone DS553AB) WB, ICC, IP Rabbit IgG

Chimeric Antibody

CAT Product Name Application Type
TAB-471CQ Anti-Human CD33 Recombinant Antibody (Vadastuximab) ELISA, IHC, FC, IP, IF, FuncS IgG1, κ

Recombinant Antibody

CAT Product Name Application Type
HPAB-0077-LSX Mouse Anti-CD33 Recombinant Antibody (HPAB-0077-LSX) ELISA, FC, SPR Mouse IgG
HPAB-0078-LSX Mouse Anti-CD33 Recombinant Antibody (HPAB-0078-LSX) ELISA, FC, SPR Mouse IgG
HPAB-0079-LSX Mouse Anti-CD33 Recombinant Antibody (HPAB-0079-LSX) ELISA, FC, SPR Mouse IgG
HPAB-0080-LSX Human Anti-CD33 Recombinant Antibody (HPAB-0080-LSX) ELISA, FC Human IgG
HPAB-0081-LSX Human Anti-CD33 Recombinant Antibody (HPAB-0081-LSX) ELISA, FC Human IgG

Fab Fragment Antibody

CAT Product Name Application Type
HPAB-M0470-YC-F(E) Human Anti-CD33 Recombinant Antibody (clone Lintuzumab); Fab Fragment FuncS Humanized Fab
HPAB-M0471-YC-F(E) Human Anti-CD33 Recombinant Antibody; Fab Fragment (HPAB-M0471-YC-F(E)) FuncS Humanized Fab
HPAB-2047LY-F(E) Human Anti-CD33 Recombinant Antibody; Fab Fragment (HPAB-2047LY-F(E)) FC, ELISA Humanized Fab
HPAB-2048LY-F(E) Human Anti-CD33 Recombinant Antibody; Fab Fragment (HPAB-2048LY-F(E)) FC, ELISA Humanized Fab
HPAB-2049LY-F(E) Human Anti-CD33 Recombinant Antibody; Fab Fragment (HPAB-2049LY-F(E)) FC, ELISA Humanized Fab

ADCC Enhanced Antibody

CAT Product Name Application Type
AFC-TAB-471CQ Afuco™ Anti-CD33 ADCC Recombinant Antibody (Vadastuximab), ADCC Enhanced ELISA, IHC, FC, IP, IF, FuncS ADCC enhanced antibody
AFC-TAB-756 Afuco™ Anti-CD33 ADCC Recombinant Antibody (Lintuzumab), ADCC Enhanced IF, IP, Neut, FuncS, ELISA, FC ADCC enhanced antibody
AFC-TAB-013 Afuco™ Anti-CD33 ADCC Recombinant Antibody (Gemtuzumab), ADCC Enhanced IF, IP, Neut, FuncS, ELISA, FC ADCC enhanced antibody

scFv Fragment Antibody

CAT Product Name Application Type
HPAB-1968LY-S(P) Human Anti-CD33 Recombinant Antibody; scFv Fragment (HPAB-1968LY-S(P)) FC Humanized scfv
HPAB-1969LY-S(P) Human Anti-CD33 Recombinant Antibody; scFv Fragment (HPAB-1969LY-S(P)) FC Humanized scfv
HPAB-1970LY-S(P) Human Anti-CD33 Recombinant Antibody; scFv Fragment (HPAB-1970LY-S(P)) FC Humanized scfv
HPAB-1971LY-S(P) Human Anti-CD33 Recombinant Antibody; scFv Fragment (HPAB-1971LY-S(P)) FC Humanized scfv
HPAB-1972LY-S(P) Human Anti-CD33 Recombinant Antibody; scFv Fragment (HPAB-1972LY-S(P)) FC Humanized scfv

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For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.

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