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Anti-CD33 (h2H12ec)-Mc-vc-PBD ADC (SGN-CD33A) (CAT#: ADC-L033)

This ADC product is composed of an anti-CD33 antibody (clone h2H12ec) conjuagated via a mc-val linker to SGD-1882 (h2H12ec-mc-val-SGD-1882). It has demonstrated a response in AML treatment by a MOA (Mechanism of Action) of Bind to DNA minor groove and cause DNA damage.


Specific Inquiry
  • Gene Expression
  • Datasheet
  • MSDS
  • COA
Subcellular Location and Protein Expression
Figure 1 IF staining of human cell line PC-3
Figure 1 IF staining of human cell line PC-3
(Immunofluorescent staining of human cell line PC-3 shows localization to nucleoplasm & plasma membrane.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/64807/1186_A4_3_selected.jpg
Figure 2 IHC staining of human lymph node
Figure 2 IHC staining of human lymph node
(Immunohistochemical staining of human lymph node shows membranous and cytoplasmic positivity in germinal center cells and non-germinal center cells.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/35832/113039_A_9_8_selected.jpg
Figure 3 IHC staining of human tonsil
Figure 3 IHC staining of human tonsil
(Immunohistochemical staining of human tonsil shows cytoplasmic positivity in germinal center cells and non-germinal center cells.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/11442/26430_A_6_8_selected.jpg
Figure 4 IF staining of human cell line RH-30
Figure 4 IF staining of human cell line RH-30
(Immunofluorescent staining of human cell line RH-30 shows localization to nucleoplasm.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/64807/1413_G6_1_red_green.jpg
Figure 5 IF staining of human cell line U-2 OS
Figure 5 IF staining of human cell line U-2 OS
(Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/64807/1200_A4_2_red_green.jpg
Normal Tissue
Figure 6 Kidney
Figure 6 Kidney
(Cells in tubules
Staining:Medium
Intensity: Strong
Quantity: <25%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/79996/170702_A_9_5.jpg
Figure 7 Lymph node
Figure 7 Lymph node
(Non-germinal center cells
Staining:Medium
Intensity: Strong
Quantity: <25%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/79996/170702_A_9_8.jpg
Figure 8 Spleen
Figure 8 Spleen
(Cells in red pulp
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/79996/170702_B_8_4.jpg
RNA Expression
Figure 9 RNA cell line category: Cell line enhanced (HEL, HL-60, HMC-1, NB-4, RPMI-8226, THP-1, U-937)
Figure 9 RNA cell line category: Cell line enhanced (HEL, HL-60, HMC-1, NB-4, RPMI-8226, THP-1, U-937)
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000105383-CD33

Specifications

  • Antibody Overview
  • Chimeric anti-CD33 monoclonal antibody, IgG1k
  • Clone
  • h2H12ec
  • Antibody Conjugation
  • Chimeric murine/human
  • Linker
  • mc-vc (maleimidocaproyl-valine-alanine)
  • Linker Class/Description
  • Peptide linkers, belonging to Enzymatically cleavable linkers, combine greater systemic stability with rapid enzymatic release of the drug in the target cell. The scission of peptidic bonds relies on lysosomal proteolytic enzymes, which have very low activities in blood due to endogenous inhibitors and the unfavorably high pH value of blood.
  • Drug
  • pyrrolobenzodiazepine (PBD) dimers SGD-1882
  • Drug Class/Description
  • Pyrrolobenzodiazepine (PBD) dimmers are highly cytotoxic DNA cross-linking agents. PBDs exert their biological activity through covalent binding via their N10-C11imine/carbinolamine moieties to the C2-amino position of a guanine residue within the minor groove of DNA.

Target

  • Introduction
  • CD33 or Siglec-3 is a transmembrane receptor expressed on cells of myeloid lineage. It is usually considered myeloid-specific, but it can also be found on some lymphoid cells.
  • Alternative Names
  • CD33; CD33 molecule; p67; SIGLEC3; SIGLEC-3

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Single-domain Antibody

CAT Product Name Application Type
NAB-321-sdAb Recombinant Anti-human CD33 VHH Single Domain Antibody WB, IP, ChiP, Neut, ELISA Llama VHH

Humanized Antibody

CAT Product Name Application Type
TAB-013 Humanized Anti-CD33 Recombinant Antibody (clone Gemtuzumab) IF, IP, Neut, FuncS, ELISA, FC, WB Humanized (from mouse) IgG4, κ
TAB-H37 Anti-Human CD33 Recombinant Antibody (Gemtuzumab Ozogamicin) ELISA, FC, IP, FuncS, IF, Neut, IHC IgG
TAB-756 Anti-CD33 Recombinant Antibody (Lintuzumab) IF, IP, Neut, FuncS, ELISA, FC, ICC IgG1 - kappa
VS3-WK1523 Human Anti-CD33 Recombinant Antibody (clone CDP-771) FC Humanized IgG4

Immunotoxin

CAT Product Name Application Type
AGTO-G008E Anti-CD33 immunotoxin (scFv)-PE Cytotoxicity assay, Function study
AGTO-G008G Anti-CD33 immunotoxin (scFv)-Gel Cytotoxicity assay, Function study
AGTO-L050E anti-CD33 immunotoxin M195 (IgG)-PE Cytotoxicity assay, Functional assay
AGTO-L050G anti-CD33 immunotoxin M195 (IgG)-Gel Cytotoxicity assay, Functional assay

Human Antibody

CAT Product Name Application Type
TAB-123LC Anti-Human CD33 Recombinant Antibody FC Human antibody
TAB-146LC Human Anti-CD33 Recombinant Antibody (TAB-146LC) ELISA Human IgG
TAB-123LC-S(P) Anti-Human CD33 Recombinant Antibody scFv Fragment FC Human antibody
TAB-146LC-S(P) Human Anti-CD33 Recombinant Antibody; scFv Fragment (TAB-146LC-S(P)) ELISA Human scFv
TAB-123LC-F(E) Anti-Human CD33 Recombinant Antibody Fab Fragment FC Human antibody

Mouse Antibody

CAT Product Name Application Type
TAB-136LC-S(P) Mouse Anti-CD33 Recombinant Antibody; scFv Fragment (TAB-136LC-S(P)) ELISA, FC Mouse scFv
TAB-137LC-S(P) Mouse Anti-CD33 Recombinant Antibody; scFv Fragment (TAB-137LC-S(P)) ELISA, FC Mouse scFv
TAB-138LC-S(P) Mouse Anti-CD33 Recombinant Antibody; scFv Fragment (TAB-138LC-S(P)) ELISA, FC Mouse scFv
TAB-139LC-S(P) Mouse Anti-CD33 Recombinant Antibody; scFv Fragment (TAB-139LC-S(P)) ELISA, FC Mouse scFv
TAB-140LC-S(P) Mouse Anti-CD33 Recombinant Antibody; scFv Fragment (TAB-140LC-S(P)) ELISA, FC Mouse scFv

Fab Glycosylation

CAT Product Name Application Type
Gly-013LC Recombinant Anti-Human CD33 Antibody (Fab glycosylation) ELISA Humanized antibody

Blocking Antibody

CAT Product Name Application Type
NEUT-341CQ Mouse Anti-CD33 Recombinant Antibody (clone WM53) FC, IHC-Fr, ICC, IP, WB, Block Mouse IgG1, κ

Rabbit Monoclonal Antibody

CAT Product Name Application Type
MOR-0553 Hi-Affi™ Rabbit Anti-CD33 Recombinant Antibody (clone DS553AB) WB, ICC, IP Rabbit IgG

Chimeric Antibody

CAT Product Name Application Type
TAB-471CQ Anti-Human CD33 Recombinant Antibody (Vadastuximab) ELISA, IHC, FC, IP, IF, FuncS IgG1, κ

Recombinant Antibody

CAT Product Name Application Type
HPAB-0307-FY Human Anti-CD33 Recombinant Antibody (clone GO) WB Humanized IgG
HPAB-676-FY Mouse Anti-CD33 Recombinant Antibody (HPAB-676-FY) FC Mouse IgG
HPAB-1929LY Human Anti-CD33 Recombinant Antibody (HPAB-1929LY) FC Humanized IgG
HPAB-1930LY Human Anti-CD33 Recombinant Antibody (HPAB-1930LY) FC Humanized IgG
HPAB-1931LY Human Anti-CD33 Recombinant Antibody (HPAB-1931LY) FC Humanized IgG

ADCC Enhanced Antibody

CAT Product Name Application Type
AFC-TAB-471CQ Afuco™ Anti-CD33 ADCC Recombinant Antibody (Vadastuximab), ADCC Enhanced ELISA, IHC, FC, IP, IF, FuncS ADCC enhanced antibody
AFC-TAB-756 Afuco™ Anti-CD33 ADCC Recombinant Antibody (Lintuzumab), ADCC Enhanced IF, IP, Neut, FuncS, ELISA, FC ADCC enhanced antibody
AFC-TAB-013 Afuco™ Anti-CD33 ADCC Recombinant Antibody (Gemtuzumab), ADCC Enhanced IF, IP, Neut, FuncS, ELISA, FC ADCC enhanced antibody

Fab Fragment Antibody

CAT Product Name Application Type
HPAB-2031LY-F(E) Human Anti-CD33 Recombinant Antibody; Fab Fragment (HPAB-2031LY-F(E)) FC, ELISA Humanized Fab
HPAB-2032LY-F(E) Human Anti-CD33 Recombinant Antibody; Fab Fragment (HPAB-2032LY-F(E)) FC, ELISA Humanized Fab
HPAB-2033LY-F(E) Human Anti-CD33 Recombinant Antibody; Fab Fragment (HPAB-2033LY-F(E)) FC, ELISA Humanized Fab
HPAB-2034LY-F(E) Human Anti-CD33 Recombinant Antibody; Fab Fragment (HPAB-2034LY-F(E)) FC, ELISA Humanized Fab
HPAB-2035LY-F(E) Human Anti-CD33 Recombinant Antibody; Fab Fragment (HPAB-2035LY-F(E)) FC, ELISA Humanized Fab

scFv Fragment Antibody

CAT Product Name Application Type
HPAB-1980LY-S(P) Human Anti-CD33 Recombinant Antibody; scFv Fragment (HPAB-1980LY-S(P)) FC Humanized scfv
HPAB-2048LY-S(P) Human Anti-CD33 Recombinant Antibody; scFv Fragment (HPAB-2048LY-S(P)) FC, ELISA Humanized scfv
HPAB-2049LY-S(P) Human Anti-CD33 Recombinant Antibody; scFv Fragment (HPAB-2049LY-S(P)) FC, ELISA Humanized scfv
HPAB-1103WJ-S(P) Mouse Anti-CD33 Recombinant Antibody; scFv Fragment (HPAB-1103WJ-S(P)) ELISA, WB Mouse scFv
HPAB-0532-YJ-S(P) Human Anti-CD33 Recombinant Antibody (clone ZJL101); scFv Fragment ELISA, Inhib Human scFv

MHC Tetramer for Cancer

CAT Product Name Application Type
MHC-CN0509 PE-A*02:01/Human CD33 (YLISGDSPV) MHC Tetramer FCM, IHC
MHC-CN0510 APC-A*02:01/Human CD33 (YLISGDSPV) MHC Tetramer FCM, IHC
MHC-CN0511 FITC-A*02:01/Human CD33 (YLISGDSPV) MHC Tetramer FCM, IHC
MHC-CN0512 A*02:01/Human CD33 (YLISGDSPV) MHC Tetramer FCM, IHC
MHC-YF358 A*02:01/Human CD33 65-73 (1Y2L) (YLISGDSPV) MHC Pentamer FCM

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