Hi-Affi™ Rabbit Anti-CD3G Recombinant Antibody (clone DS550AB)
CAT#: MOR-0550
Recombinant Rabbit anti-CD3 monoclonal antibody for IHC, excellent for producing superior results. Hi-Affi™ antibodies are developed from different sources, such as B cells of immunized animals, hybridoma, phage display library. The application of recombinant technology avoids the traditional ascitic fluid approach, which achieves the antibody generation in a humane way with much larger scale, greater specificity and sensitivity, and not susceptible to cell-line drift or lot-to-lot variation.
Gene Expression
Subcellular Location and Protein Expression
Figure 1 IF staining of human cell line K-562
Immunofluorescent staining of human cell line K-562 shows localization to plasma membrane & cytosol.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/38494/1892_A4_2_selected.jpg
Normal Tissue
Figure 2 Cerebral cortex
Neuronal cells
Staining:Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/17520/39923_B_8_5.jpg
Normal Tissue
Figure 3 Testis
Cells in seminiferous ducts
Staining:Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous nuclear
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/17520/39923_A_5_6.jpg
Normal Tissue
Figure 4 Bone marrow
Hematopoietic cells
Staining:Medium
Intensity: Strong
Quantity: <25%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/17520/39923_B_6_4.jpg
Normal Tissue
Figure 5 Lymph node
Germinal center cells
Staining:Medium
Intensity: Strong
Quantity: <25%
Location: Cytoplasmic/membranous
Non-germinal center cells
Staining:Medium
Intensity: Strong
Quantity: <25%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/17520/39923_A_7_8.jpg
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Specifications
- Immunogen
- A synthetic 13-mer peptide corresponding to aa 156-168 of the epsilon chain of human CD3 protein.
- Host Species
- Rabbit
- Derivation
- Phage display library screening
- Type
- Rabbit IgG
- Species Reactivity
- Human
- Clone
- DS550AB
- Applications
- IHC
- Conjugate
- Unconjugated
Product Property
- Purification
- Purified with Absolute Mag™ Protein A Agarose
- Purity
- >95% as determined by analysis by SDS-PAGE
- Format
- Liquid
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Target
- Alternative Names
- CD3g Molecule; CD3g Molecule, Gamma (CD3-TCR Complex); CD3g Antigen, Gamma Polypeptide (TiT3 Complex); T-Cell Receptor T3 Gamma Chain; T3G; T-Cell Antigen Receptor Complex, Gamma Subunit Of T3; CD3g Molecule, Epsilon (CD3-TCR Complex); CD3g Antigen; CD3-GAMMA; IMD17
- Gene ID
- 917
- UniProt ID
- P09693
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Downloadable Resources
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
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| MOB-2778z | Mouse Anti-CD3G Recombinant Antibody (clone 26G7) | WB, ELISA, FC | Mouse IgG1 |
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| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-0559 | Hi-Affi™ Rabbit Anti-CD3G Recombinant Antibody (clone DS559AB) | ICC, IHC-P, WB | Rabbit IgG |
| CAT | Product Name | Application | Type |
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| CAT | Product Name | Application | Type |
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| VS-0425-YC36 | Recombinant Anti-CD3G Vesicular Antibody, EV Displayed (VS-0425-YC36) | ELISA, FC, Cell-uptake |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0525-XY1176 | Anti-CD3G Immunohistochemistry Kit | IHC | |
| VS-0525-XY1177 | Anti-Mouse CD3G Immunohistochemistry Kit | IHC |
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