Human Anti-CD40 Recombinant Antibody (clone S2C6) (CAT#: HPAB-0166-YC)

Figure 1 Activation potential of a panel of anti-CD40 antibodies measured as [methyl-³H]thymidine uptake of tonsilla B cells after addition of anti-CD40 monoclonal antibodies (MoAbs).

The experiments were performed using 1 µg/ml in three independent experiments.

Hager, A. C. M., Ellmark, P., Borrebaeck, C. A. K., & Furebring, C. (2003). Affinity and Epitope Profiling of Mouse Anti‐CD40 Monoclonal Antibodies. Scandinavian journal of immunology, 57(6), 517-524.

Figure 2 Ability of anti-CD40 mAb to co-stimulate anti-IgM-induced human B-cell proliferation.

Human tonsillar B cells (4 x 10⁴/well) were cultured in 200 ul in microwells in the presence of anti-IgM coupled to Sepharose beads (5 yg/ml) (a) or in the presence of anti-IgM plus rIL-2 (100 U/ml) (b). Varying concentrations of the anti-CD40 mAb S2C6,5D12, 3C6 or 3A8 were added and [³H]thymidine incorporation was estimated at day 3 after 18 hr pulsing. Data presented in (a) are means derived from experiments with B-cell preparations from three different donors with duplicate incubations. Data in (b) are means of duplicate incubations from one experiment out of two with comparable results.

Kwekkeboom, J., De Boer, M., Tager, J. M., & De Groot, C. (1993). CD40 plays an essential role in the activation of human B cells by murine EL4B5 cells. Immunology, 79(3), 439.

Figure 3 Effect of anti-CD40 mAb G28.5 and S2C6 on EL4B5-induced human B-cell proliferation.

Human tonsillar B cells were cultured together with irradiated EL4B5 cells. Varying concentrations of anti-CD40 mAb G28.5 and S2C6 were added. As an isotype-matched control mAb MOPC-21 (IgGl) was used. Data are means + SD derived from three experiments with duplicate incubations. Actual [³H]thymidine incorporations found in the absence of antibody were 6982 + 1729, 4362 + 1020 and 14,809 2791 c.p.m. respectively

Kwekkeboom, J., De Boer, M., Tager, J. M., & De Groot, C. (1993). CD40 plays an essential role in the activation of human B cells by murine EL4B5 cells. Immunology, 79(3), 439.