Human Anti-CD40 Recombinant Antibody (clone S2C6) (CAT#: HPAB-0166-YC)
CD40 is a cell surface phosphorylated glycoprotem that is expressed on a variety of cell types, including B cells, B cell malignancies, follicular dendritic cells, basal epithelial cells, and carcinomas. CD40 binds CD40 ligand ("CD40L").
Specific Inquiry
Activ
![Figure 1 Activation potential of a panel of anti-CD40 antibodies measured as [methyl-³H]thymidine uptake of tonsilla B cells after addition of anti-CD40 monoclonal antibodies (MoAbs).](https://img.creativebiolabs.net/productimages/HPAB-0166-YC-1.png)
Figure 1 Activation potential of a panel of anti-CD40 antibodies measured as [methyl-³H]thymidine uptake of tonsilla B cells after addition of anti-CD40 monoclonal antibodies (MoAbs).
The experiments were performed using 1 µg/ml in three independent experiments.
Hager, A. C. M., Ellmark, P., Borrebaeck, C. A. K., & Furebring, C. (2003). Affinity and Epitope Profiling of Mouse Anti‐CD40 Monoclonal Antibodies. Scandinavian journal of immunology, 57(6), 517-524.
Stim
Figure 2 Ability of anti-CD40 mAb to co-stimulate anti-IgM-induced human B-cell proliferation.
Human tonsillar B cells (4 x 10⁴/well) were cultured in 200 ul in microwells in the presence of anti-IgM coupled to Sepharose beads (5 yg/ml) (a) or in the presence of anti-IgM plus rIL-2 (100 U/ml) (b). Varying concentrations of the anti-CD40 mAb S2C6,5D12, 3C6 or 3A8 were added and [³H]thymidine incorporation was estimated at day 3 after 18 hr pulsing. Data presented in (a) are means derived from experiments with B-cell preparations from three different donors with duplicate incubations. Data in (b) are means of duplicate incubations from one experiment out of two with comparable results.
Kwekkeboom, J., De Boer, M., Tager, J. M., & De Groot, C. (1993). CD40 plays an essential role in the activation of human B cells by murine EL4B5 cells. Immunology, 79(3), 439.
FuncS
Figure 3 Effect of anti-CD40 mAb G28.5 and S2C6 on EL4B5-induced human B-cell proliferation.
Human tonsillar B cells were cultured together with irradiated EL4B5 cells. Varying concentrations of anti-CD40 mAb G28.5 and S2C6 were added. As an isotype-matched control mAb MOPC-21 (IgGl) was used. Data are means + SD derived from three experiments with duplicate incubations. Actual [³H]thymidine incorporations found in the absence of antibody were 6982 + 1729, 4362 + 1020 and 14,809 2791 c.p.m. respectively
Kwekkeboom, J., De Boer, M., Tager, J. M., & De Groot, C. (1993). CD40 plays an essential role in the activation of human B cells by murine EL4B5 cells. Immunology, 79(3), 439.
Specifications
- Immunogen
- A human B cell tumor line: Ramos, HS Sultan or IM-9 cells
- Host Species
- Human
- Type
- Human IgG
- Specificity
- Human CD40
- Species Reactivity
- Human
- Clone
- S2C6
- Applications
- FC, FuncS
- Related Disease
- Cancer, Inflammatory diseases, Deficiencies of the immune system
Product Property
- Purity
- >95% as determined by SDS-PAGE and HPLC analysis
- Buffer
- PBS
- Preservative
- No preservatives
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Target
Related Resources
Asthma
Systemic Lupus Erythematosus
Primary Immunodeficiency
Allograft Rejection
Autoimmune Thyroid Disease
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
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scFv Fragment Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| AGTO-G079E | Anti-CD40 immunotoxin G28-5 (scFv)-PE | Cytotoxicity assay, Function study |
Customer Reviews and Q&As
Customer Review
Q&As
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Fast shipping
The shipping was fast, and the product arrived in perfect condition. We appreciate the swift service and careful packaging to ensure quality. -
Easy to use
The product is easy to use and comes with clear instructions, which is particularly helpful when training new lab members. -
Excellent binding
We've observed superb binding affinity with this antibody product, leading to more accurate detections and measurements in our experiments.
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For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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