Mouse Anti-CD5 Recombinant Antibody (clone 34)
CAT#: HPAB-0029-LSX
Recombinant monoclonal antibody to CD5. Clone 34 is a mouse monoclonal antibody that can be potentially used in the treatment of Chronic lymphocytic leukaemia (CLL).
Published Data
Gene Expression
FC
Figure 1 Overlay histograms showing simultaneous binding of four antibodies directed against non overlapping epitopes on the extra cellular domain of CD5 as determined by Flow cytometry.
The CEM cells were stained as follows (Anti-CD5 antibody, Line, Mean Flourescence Intensity): No antibody, Solid, 7,29; Clone 12, Dash, 479,33; Clone 14, Dot, 636,65; Clone 17, DashDot, 396,29; Clone 34, DashDotDot, 181,14; Mix of Clone 12,14,17 and 34, Solid with Grey fill, 1292,72.
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Normal Tissue
Figure 1 Testis
Spermatogonia cells
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/43416/101306_A_5_6.jpg
Normal Tissue
Figure 2 Lymph node
Germinal center cells
Staining: Medium
Intensity: Strong
Quantity: <25%
Location: Cytoplasmic/membranous
Non-germinal center cells
Staining: High
Intensity: Strong
Quantity: 75%-25%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/43416/101306_A_7_8.jpg
Normal Tissue
Figure 3 Tonsil
Germinal center cells
Staining: Medium
Intensity: Strong
Quantity: <25%
Location: Cytoplasmic/membranous
Non-germinal center cells
Staining: High
Intensity: Strong
Quantity: 75%-25%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/43416/101306_A_5_8.jpg
RNA Expression
Figure 4 RNA cell line category: Group enriched (JURKAT, MOLT-4)
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000110448-CD5
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Specifications
- Immunogen
- CD5-human growth hormone (hGH) fusion protein
- Host Species
- Mouse
- Type
- Mouse IgG
- Specificity
- Human CD5
- Species Reactivity
- Human
- Clone
- Clone 34
- Applications
- ELISA, WB, FC
- Related Disease
- Chronic lymphocytic leukaemia
Product Property
- Purity
- >95% as determined by SDS-PAGE and HPLC analysis
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- PBS
- Preservative
- No preservatives
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- This antibody has been tested for use in Flow Cytometry.
Target
REVIEWS AND Q&AS
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Fit for Use in Applications Sensitive to Light
This antibody, when wrapped in foil, exhibited no evidence of degradation and functioned well in light-sensitive experiments.
Examined Lot
Every new lot outperformed the previous one, exhibiting dependable performance over time.
Hardly Any Cross-Reactivity Was Noticed
The response was quite precise to the intended target, and there was no discernible cross-reactivity.
Q&As
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What distinguishes chimeric from humanized antibodies?
A: Chimeric antibodies combine bigger sections from both human and non-human sources, whereas humanized antibodies are largely human with only the antigen-binding sites coming from a non-human species.
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Does the antibody require a certain way to be disposed of?
A: To protect the environment and maintain safety, dispose of biological products in accordance with local laws and regulations.
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How can I increase the sensitivity of my assay?
A: Elevate the concentration of the antibody, use a more sensitive detection method, or adjust the assay parameters.
View the frequently asked questions answered by Creative Biolabs Support.
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Downloadable Resources
Download resources about recombinant antibody development and antibody engineering to boost your research.
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
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