Human Anti-CD83 Recombinant Antibody; Fab Fragment (TAB-353LC-F(E)) (CAT#: TAB-353LC-F(E))
This product is a recombinant Human antibody Fab fragment that recognizes Cluster of differentiation 83. The antibody was purified by affinity chromatography.
We specialize in custom recombinant antibody production, offering seamless execution from provided sequences to high-quality antibody deliverables, ensuring optimal yield and purity.
Figure 1 Human Anti-CD83 Recombinant Antibody Fab Fragment (TAB-353LC-F(E)) in SDS-PAGE
SDS-PAGE analysis of TAB-353LC-F(E) inreduced (Lane 1) and non-reduced (Lane 2) conditions. Gel stained for 30 minutes with Coomassie Blue.
The purity of TAB-353LC-F(E) was greater than 95% as determined by SDS-PAGE.
Figure 2 Human Anti-CD83 Recombinant Antibody Fab Fragment (TAB-353LC-F(E)) in HPLC
The purity of TAB-353LC-F(E) was greater than 95% as determined by SEC-HPLC.
Figure 3 Human Anti-CD83 Recombinant Antibody Fab Fragment (TAB-353LC-F(E)) in WB
Western blot analysis of TAB-353LC-F(E) was performed by loading recombinant human CD83 protein.
TAB-353LC-F(E) incubation concentration: 2 ng/μL.
The secondary antibody: HRP-goat anti-human IgG
Lane 1: Reduced Antigen (0.6 μg)
Figure 4 Human Anti-CD83 Recombinant Antibody Fab Fragment (TAB-353LC-F(E)) in ELISA
ELISA analysis of TAB-353LC-F(E) was performed by coating with recombinant human CD83 protein. Then blocked with BSA, and incubated with Human Anti-CD83 Recombinant Antibody Fab Fragment (TAB-353LC-F(E)). The Goat Anti-Human IgG-HRP as a secondary antibody. Detection was performed using TMB substrate and stopped with sulfuric acid. The absorbances were read on a spectrophotometer at 450 nm.
Specifications
- Host Species
- Human
- Type
- Human Fab
- Specificity
- Human CD83
- Species Reactivity
- Human
- Applications
- WB, SPR
- Related Disease
- Inflammatory disease, Autoimmune disease
Product Property
- Purity
- >95% as determined by SDS-PAGE
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- PBS
- Preservative
- No preservatives
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Target
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
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See other products for "CD83"
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CAT | Product Name | Application | Type |
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MOB-1541z | Mouse Anti-CD83 Recombinant Antibody (clone 36G7) | WB, ELISA, FC, IF, IHC, IP | Mouse IgG2a, κ |
MOB-1606MZ | Recombinant Mouse Anti-Human CD83 Antibody (clone 2I5c) | IHC-P | Mouse antibody |
VS3-XY422 | Mouse Anti-CD83 Recombinant Antibody, FITC (clone 4AHB15e) | FC, IF | Mouse IgG1 |
VS-0723-WK126 | Human Anti-CD83 Recombinant Antibody (VS-0723-WK126) | FC | Chimeric (rabbit/human) IgG1 |
VS-0724-YC29 | Rabbit Anti-CD83 Recombinant Antibody (clone 20) | ELISA, Inhib | Rabbit IgG |
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