Recombinant Anti-Human CD8A VHH Single Domain Antibody
CAT#: NABG-041
Anti-Human CD8A VHH Single Domain Antibody is a recombinant protein produced in E. coli.
Gene Expression
Subcellular Location and Protein Expression
Figure 1 IF staining of human cell line SH-SY5Y
Immunofluorescent staining of human cell line SH-SY5Y shows localization to plasma membrane.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/37756/1373_A3_1_selected.jpg
Subcellular Location and Protein Expression
Figure 2 IF staining of human cell line RH-30
Immunofluorescent staining of human cell line RH-30 shows localization to plasma membrane.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/37756/1492_D3_2_blue_red_green.jpg
Subcellular Location and Protein Expression
Figure 3 IF staining of human cell line U-2 OS
Immunofluorescent staining of human cell line U-2 OS shows localization to plasma membrane.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/37756/1377_A3_1_blue_red_green.jpg
Normal Tissue
Figure 4 Lymph node
Germinal center cells
Staining:Medium
Intensity: Strong
Quantity: <25%
Location: Cytoplasmic/membranous nuclear
Non-germinal center cells
Staining:High
Intensity: Strong
Quantity: 75%-25%
Location: Cytoplasmic/membranous nuclear
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/12/97_A_7_8.jpg
RNA Expression
Figure 5 RNA cell line category: Cell line enhanced (JURKAT, MOLT-4, RPMI-8226)
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000153563-CD8A
❮
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Specifications
- Immunogen
- Human CD8a molecule
- Host Species
- Llama
- Type
- Llama VHH
- Species Reactivity
- Human
- Molecule Class
- VHH Single Domain Antibody
- Applications
- Can be useful in applications such as: Immunohistochemistry; Flow Cytometry; Competitive assay; Functional Studies
Product Property
- Purification
- Purified on Immobilized Metal Affinity Chromatography (IMAC).
- Purity
- >95% as determined by analysis by SDS-PAGE
- Storage
- Store at -20°C. Open under aseptic conditions.
Target
- Alternative Names
- CD8A; CD8a molecule; CD8; MAL; p32; Leu2; T-cell surface glycoprotein CD8 alpha chain; T8 T-cell antigen; T cell co-receptor; OKT8 T-cell antigen; T-cell antigen Leu2; Leu2 T-lymphocyte antigen; CD8 antigen, alpha polypeptide (p32); T-lymphocyte different
- Gene ID
- 925
- UniProt ID
- P01732
REVIEWS AND Q&AS
CITATIONS
RESOURCES
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Maneuverable with Different Detergents
It showed consistent performance even in the presence of mild detergents used in membrane protein studies.
Zero Aggregation Issues
No aggregation noticed even with prolonged storage. Ensures consistent results and reliability.
Detailed Validation Support
Validation steps suggested by the supplier were thorough and effective, making adoption into new assays straightforward.
Q&As
-
Does antibody lot-to-lot variability influence experimental results?
A: Lot-to-lot fluctuations may have an effect on repeatability. By using aliquots from the same lot or by confirming each new lot, this risk can be minimized.
-
How do I choose an antibody for Western blotting versus immunohistochemistry?
A: Select antibodies that have been validated for the specific application, as different methods have varying requirements for epitope recognition and secondary detection.
-
Can I use antibodies in serum-free medium?
A: Yes, but remember that some antibodies may function better in serum. Check your antibody under the specific assay settings.
View the frequently asked questions answered by Creative Biolabs Support.
Cite This Product
To accurately reference this product in your publication, please use the following citation information:
(Creative Biolabs Cat# NABG-041, RRID: AB_3111554)
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Downloadable Resources
Download resources about recombinant antibody development and antibody engineering to boost your research.
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
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| CAT | Product Name | Application | Type |
|---|---|---|---|
| NAB-369-VHH | Recombinant Anti-Mouse Cd8a VHH Single Domain Antibody | IHC, ICC, IP, ChiP, Neut | Llama VHH |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-184-S(P) | Recombinant Anti-human CD8A Antibody scFv Fragment | IP, IF, Biosensors, FuncS | scFv |
| MHH-184-S(P) | Recombinant Human Anti-human CD8A Antibody scFv Fragment | ELISA, WB, FuncS | scFv |
| PFBZ-144 | Rat Anti-CD8A Recombinant Antibody (clone YTS 105.18); Fab Fragment | Neut | Rat Fab |
| FAMAB-0009-CN-S(P) | Mouse Anti-CD8A Recombinant Antibody (clone CT8); scFv Fragment | FC | Mouse scFv |
| HPAB-S0183-YC-S(P) | Rat Anti-Cd8a Recombinant Antibody (clone YTS105.18); scFv Fragment | ELISA | Rat scFv |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| PABZ-144 | Rat Anti-CD8A Recombinant Antibody (clone YTS 105.18) | Neut | Rat IgG |
| MRO-1761-CN | Rabbit Anti-CD8A Polyclonal Antibody (MRO-1761-CN) | WB, IF, IHC, FC | Rabbit IgG |
| FN-073CQ | Mouse Anti-CD8A Recombinant Antibody (clone 2.43) | FC, IP, Depletion | Mouse IgG2b |
| ZG-0686F | Mouse Anti-CD8A Recombinant Antibody (clone 17D8) | FC | Mouse IgG1 |
| ZG-0690F | Mouse Anti-CD8A Antibody (clone 17D8/BHKG-2) | FC | Mouse IgG1 |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| NEUT-428CQ | Mouse Anti-CD8A Recombinant Antibody (clone UCHT4) | FC, IHC-Fr, IHC-P, IP, Separation, Block | Mouse IgG2a, κ |
| NEUT-431CQ | Rat Anti-CD8A Recombinant Antibody (clone 53-6.7) | ICC, IF, FC, IHC-Fr, IP, FuncS, Block | Rat IgG2a, κ |
| NEUT-432CQ | Rat Anti-CD8A Recombinant Antibody (clone MAB0811) | Block, Depletion | Rat IgG2a |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| NEUT-429CQ | Mouse Anti-CD8A Recombinant Antibody (clone CT-8) | Neut, FC, IHC, IHC-Fr, IHC-P, IP, ICC | Mouse IgG1, κ |
| NEUT-430CQ | Mouse Anti-CD8A Recombinant Antibody (clone LT8) | WB, Neut | Mouse IgG1 |
| NEUT-433CQ | Mouse Anti-CD8A Recombinant Antibody (clone 76-2-11) | Neut, FC, IHC-Fr, IHC-P, IP | Mouse IgG2a, κ |
| NEUT-434CQ | Mouse Anti-Cd8a Recombinant Antibody (clone OX-8) | WB, Neut, FC, IHC, IHC-Fr, IHC-P, IP, CyTOF® | Mouse IgG1 |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-0582 | Hi-Affi™ Rabbit Anti-CD8A Recombinant Antibody (clone DS582AB) | IHC | Rabbit IgG |
| MOR-0589 | Hi-Affi™ Rabbit Anti-CD8A Recombinant Antibody (clone DS589AB) | IHC-P | Rabbit IgG |
| MOR-0590 | Hi-Affi™ Rabbit Anti-CD8A Recombinant Antibody (clone DS590AB), PE | FC | Rabbit IgG |
| MOR-0057-FY | Rabbit Anti-CD8A Recombinant Antibody (clone AFY0028) | ICC, IF, IHC-P, WB | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| FAMAB-0041-CN | Mouse Anti-CD8A Recombinant Antibody (clone 13C5) | WB, IP, IF | Mouse IgM |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| FAMAB-0009-CN-F(E) | Mouse Anti-CD8A Recombinant Antibody (clone CT8); Fab Fragment | FC | Mouse Fab |
| FAMAB-0010-CN-F(E) | Mouse Anti-CD8A Recombinant Antibody (clone EP72); Fab Fragment | FC | Mouse Fab |
| FAMAB-0011-CN-F(E) | Mouse Anti-CD8A Recombinant Antibody (clone 3-298); Fab Fragment | FC | Mouse Fab |
| FAMAB-0037-CN-F(E) | Mouse Anti-CD8A Recombinant Antibody (clone 6G5); Fab Fragment | WB, IP, IF | Mouse Fab |
| FAMAB-0038-CN-F(E) | Mouse Anti-CD8A Recombinant Antibody (clone 1C9); Fab Fragment | WB, IP, IF | Mouse Fab |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-1024-XY114 | Mouse Anti-NHP CD8A Recombinant Antibody (clone T8/209) | ELISA | Mouse IgG1, kappa |
| VS-1024-XY112 | Rat Anti-NHP CD8A Recombinant Antibody (clone YTC182.20) | FC | Rat IgG2b |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0225-XY78 | CytoStream™ Mouse Anti-CD8A Recombinant Antibody (VS-0225-XY78) | FC | Mouse IgG2a, kappa |
| VS-0225-XY79 | CytoStream™ Mouse Anti-CD8A Recombinant Antibody (clone SK1) | FC | Mouse IgG1, kappa |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0325-XY375 | Anti-CD8A Immunohistochemistry Kit | IHC | |
| VS-0525-XY1238 | Anti-Mouse CD8A Immunohistochemistry Kit | IHC | |
| VS-0525-XY1237 | Anti-Human CD8A Immunohistochemistry Kit | IHC | |
| VS-0525-XY1240 | Anti-Rat CD8A Immunohistochemistry Kit | IHC |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0425-YC377 | Recombinant Anti-CD8A Vesicular Antibody, EV Displayed (VS-0425-YC377) | ELISA, FC, Neut, Cell-uptake |
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