Hi-Affi™ Rabbit Anti-CD8B Recombinant Antibody (clone DS591AB)
CAT#: MOR-0591
Recombinant Rabbit anti-CD8B monoclonal antibody for ICC/IF, WB, excellent for producing superior results. Hi-Affi™ antibodies are developed from different sources, such as B cells of immunized animals, hybridoma, phage display library. The application of recombinant technology avoids the traditional ascitic fluid approach, which achieves the antibody generation in a humane way with much larger scale, greater specificity and sensitivity, and not susceptible to cell-line drift or lot-to-lot variation.
Gene Expression
Subcellular Location
Figure 1 IF staining of human cell line CACO-2
Immunofluorescent staining of human cell line CACO-2 shows localization to plasma membrane.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/29164/1526_E8_1_selected.jpg
Normal Tissue
Figure 2 High expression in lymph node.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/4353/165972_A_9_8_val_selected.jpg
Normal Tissue
Figure 3 Low expression in liver.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/4353/165972_A_9_4_val_selected.jpg
Normal Tissue
Figure 4 Lymph node
Germinal center cells Staining: Medium Intensity: Strong Quantity: <25% Location: Cytoplasmic/ membranous Non-germinal center cells Staining: Medium Intensity: Strong Quantity: <25% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/4353/165972_A_9_8.jpg
RNA Expression
Figure 5 RNA cell line category: Cell line enriched (RPMI-8226)
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000172116-CD8B
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Specifications
- Immunogen
- A synthetic peptide corresponding to residues in the extracellular domain of Human CD8B.
- Host Species
- Rabbit
- Derivation
- Phage display library
- Type
- Rabbit IgG
- Specificity
- Human CD8B
- Species Reactivity
- Human
- Clone
- DS591AB
- Applications
- ICC, IF, WB
- Conjugate
- Unconjugated
Product Property
- Purification
- Tissue culture supernatant
- Concentration
- Please refer to the vial label for the specific concentration.
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Target
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Downloadable Resources
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
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| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-1604MZ | Recombinant Mouse Anti-Rat CD8B Antibody (clone 452) | FC, IHC-P, IP, WB | Mouse antibody |
| FAMAB-0012-CN | Mouse Anti-CD8B Recombinant Antibody (clone EP42) | FC | Mouse IgG |
| HPAB-S0182-YC | Rat Anti-Cd8b1 Recombinant Antibody (clone YTS156.7.7) | Neut | Rat IgG |
| FAMAB-1681CQ | Mouse Anti-CD8B Recombinant Antibody (clone YTS 156.7.7) | FC, IHC | Mouse IgG2a |
| VS-0224-XY29 | Monkey Anti-CD8B Recombinant Antibody (VS-0224-XY29) | Depletion, In vivo assay | Monkey IgG1 |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| NEUT-435CQ | Rat Anti-CD8B Recombinant Antibody (clone 53-5.8) | Depletion, Block, IF | Rat IgG1 |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| FAMAB-0012-CN-S(P) | Mouse Anti-CD8B Recombinant Antibody (clone EP42); scFv Fragment | FC | Mouse scFv |
| HPAB-S0182-YC-S(P) | Rat Anti-Cd8b1 Recombinant Antibody (clone YTS156.7.7); scFv Fragment | Neut | Rat scFv |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| FAMAB-0012-CN-F(E) | Mouse Anti-CD8B Recombinant Antibody (clone EP42); Fab Fragment | FC | Mouse Fab |
| HPAB-S0182-YC-F(E) | Rat Anti-Cd8b1 Recombinant Antibody (clone YTS156.7.7); Fab Fragment | Neut | Rat Fab |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0225-XY80 | CytoStream™ Mouse Anti-CD8B Recombinant Antibody (clone 2ST8.5H7) | FC | Mouse IgG2a, kappa |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0325-FY185 | Rat Anti-CD8B (clone YTS156.7.7) scFv-Fc Chimera | FC | Rat IgG2b, scFv-Fc |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0425-YC445 | Recombinant Anti-CD8B Vesicular Antibody, EV Displayed (VS-0425-YC445) | ELISA, FC, Neut, Cell-uptake |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0525-XY1242 | Anti-CD8B Immunohistochemistry Kit | IHC | |
| VS-0525-XY1243 | Anti-Human CD8B Immunohistochemistry Kit | IHC | |
| VS-0525-XY1244 | Anti-Rat CD8B Immunohistochemistry Kit | IHC |
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