What is the target and clone ID of this antibody?

The target is CDH1 (E-cadherin) and the clone ID is VMC174. It is a mouse monoclonal antibody (IgG2a) that specifically binds to human E-cadherin.

Can this antibody be used for blocking and immunoprecipitation?

Yes, this antibody can neutralize the bioactivity of E-cadherin (Block) and is also validated for Immunoprecipitation (IP), Western Blot (WB), and ELISA.

Mouse Anti-CDH1 Recombinant Antibody (VMC174)

CAT#: NEUT-443CQ

This antibody is a mouse monoclonal antibody that binds specifically to E-cadherin, and it can neutralize the bioactivity of E-cadherin.

Gene Expression

Subcellular Location Figure 1 IF staining of human cell line A-431

Immunofluorescent staining of human cell line A-431 shows localization to plasma membrane, the Golgi apparatus & cell junctions.

* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/28364/672_E7_2_selected.jpg

Normal Tissue Figure 2 ParaThyroid gland

High expression

* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/4812/158154_A_3_8_val_selected.jpg

Normal Tissue Figure 3 Colon

Endocrine cells Staining: High Intensity: Strong Enterocytes Staining: High Intensity: Strong Quantity:>75% Enterocytes - Microvilli Staining: High Intensity: Strong Goblet cells Staining: High Intensity: Strong Quantity:>75%

* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/72856/157200_A_9_3.jpg

Normal Tissue Figure 4 Liver

Cholangiocytes Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous Hepatocytes Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous

* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/72856/157200_A_7_4.jpg

Normal Tissue Figure 5 Kidney

Bowman's capsule Staining: Medium Intensity: Moderate Quantity:>75% Collecting ducts Staining: High Intensity: Strong Quantity:>75% Distal tubules Staining: High Intensity: Strong Quantity:>75%

* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/72856/157200_A_7_5.jpg

Normal Tissue Figure 1 Urinary bladder

Urothelial cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous

* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/72856/157200_A_5_5.jpg

RNA Expression Figure 1 RNA cell line category: Cell line enhanced (BEWO, CACO-2, CAPAN-2, HaCaT, hTCEpi, MCF7, OE19, RT4, T-47d)

Cell lines ordered by descending RNA expression order.

* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000039068-CDH1

❮

❯

Specifications

Immunogen
Human placenta.

Host Species
Mouse

Type
Mouse IgG2a

Specificity
Human CDH1

Species Reactivity
Human

Clone
VMC174

Applications
ELISA, IP, WB, ICC, Block, FC, IHC-P

Conjugate
Unconjugated

Product Property

Purity
>95% as determined by SDS-PAGE

Concentration
Please refer to the vial label for the specific concentration.

Storage
Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Target

Alternative Names
CDH1; cadherin 1; UVO; CDHE; ECAD; LCAM; Arc-1; CD324

Gene ID
999

UniProt ID
P12830

REVIEWS AND Q&AS CITATIONS RESOURCES DOWNLOADS RELATED PRODUCTS

Inquiry

Navs

Customer Review

There are currently no Customer reviews or questions for NEUT-443CQ. Click the button above to contact us or submit your feedback about this product.

Breast cancer biomarkers at key points during disease progression

Robust E-Cadherin Signal

The VMC174 clone is excellent for detecting CDH1. We have used it in Western Blot and IP with great success. It specifically binds to human CDH1 and provides strong signals, helping us study cell adhesion complexes.

Effective Blocking Agent

We utilized this antibody in blocking assays to neutralize E-cadherin. It worked as expected. The antibody is also validated for IHC-P and Flow Cytometry, proving its high specificity and utility for our cancer research projects.

Q&As

What is the target and clone ID of this antibody?

A: The target is CDH1 (E-cadherin) and the clone ID is VMC174. It is a mouse monoclonal antibody (IgG2a) that specifically binds to human E-cadherin.
Can this antibody be used for blocking and immunoprecipitation?

A: Yes, this antibody can neutralize the bioactivity of E-cadherin (Block) and is also validated for Immunoprecipitation (IP), Western Blot (WB), and ELISA.

View the frequently asked questions answered by Creative Biolabs Support.

Submit Your Publication

Published with our product? Submit your paper and receive a 10% discount on your next order! Share your research to earn exclusive rewards.

Downloadable Resources

Download resources about recombinant antibody development and antibody engineering to boost your research.

Product Notes

This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production

See more details about Hi-Affi™ recombinant antibody benefits.

Datasheet

Datasheet - NEUT-443CQ.pdf

MSDS

MSDS - NEUT-443CQ.pdf

COA

Certificate of Analysis Lookup

To download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.

Lot Number:

See other products for "CDH1"

Select a product category from the dropdown menu below to view related products.

CAT	Product Name	Application	Type
MOB-1472z	Mouse Anti-CDH1 Recombinant Antibody (clone 25C1)	WB, FC, ICC, IF, IHC	Mouse IgG1, κ

CAT	Product Name	Application	Type
TAB-1406CL	Anti-Human CDH1 Recombinant Antibody (SC10.19)	ELISA

CAT	Product Name	Application	Type
TAB-1407CL	Anti-Human CDH1 Recombinant Antibody (SC10.6)	ELISA

CAT	Product Name	Application	Type
TAB-1408CL	Anti-Human CDH1 Recombinant Antibody (SC10.178)	Cyt, FuncS

CAT	Product Name	Application	Type
TAB-1409CL	Anti-Human CDH1 Recombinant Antibody (SC10.15)	ELISA

CAT	Product Name	Application	Type
TAB-1410CL	Anti-Human CDH1 Recombinant Antibody (SC10.17)	Inhib

CAT	Product Name	Application	Type
TAB-1411CL	Anti-Human CDH1 Recombinant Antibody (hSC10.17)	Cyt, FuncS, Inhib	Humanized antibody

CAT	Product Name	Application	Type
TAB-1411CL-S(P)	Anti-Human CDH1 Recombinant Antibody scFv Fragment (hSC10.17)	ELISA	Humanized antibody

CAT	Product Name	Application	Type
TAB-1411CL-F(E)	Anti-Human CDH1 Recombinant Antibody Fab Fragment (hSC10.17)	ELISA	Humanized antibody

CAT	Product Name	Application	Type
MOB-0303MZ	Recombinant Mouse Anti-Human E-Cadherin Antibody (clone NCI-39)	IHC	Mouse antibody

CAT	Product Name	Application	Type
BRD-0111MZ	Chicken Anti-CDH1 Polyclonal IgY	WB	Chicken antibody

CAT	Product Name	Application	Type
NEUT-440CQ	Rat Anti-CDH1 Recombinant Antibody (clone DECMA-1)	FC, FuncS, Neut, WB, IF, IP	Rat IgG1, κ

CAT	Product Name	Application	Type
NEUT-441CQ	Rat Anti-Cdh1 Recombinant Antibody (NEUT-441CQ)	IHC-Fr, Block, ICC, IF, WB	Rat IgG1

CAT	Product Name	Application	Type
NEUT-442CQ	Mouse Anti-CDH1 Recombinant Antibody (VMC173)	ELISA, FC, ICC, IF, IHC, IP, WB, IHC-P, IHC-Fr, Block	Mouse IgG1

CAT	Product Name	Application	Type
MOR-0611	Hi-Affi™ Rabbit Anti-CDH1 Recombinant Antibody (clone DS611AB)	ICC, IF, IHC-P, IHC-P, IP, WB	Rabbit IgG

CAT	Product Name	Application	Type
MOR-4569	Hi-Affi™ Rabbit Anti-CDH1 Recombinant Antibody (clone TH81DS)	WB, IF, ICC, FC	Rabbit IgG

CAT	Product Name	Application	Type
MRO-0506-CN	Rabbit Anti-CDH1 Recombinant Antibody (clone CBACN-205)	WB, IF, IHC, IP, FC	Rabbit IgG

CAT	Product Name	Application	Type
MRO-0514-CN	Mouse Anti-CDH1 Recombinant Antibody (clone 3-F9)	WB, IF, IHC, FC	Mouse IgG2b

CAT	Product Name	Application	Type
MRO-0515-CN	Mouse Anti-CDH1 Recombinant Antibody (clone A0-G11)	WB, IF, IHC, FC	Mouse IgG1

CAT	Product Name	Application	Type
HPAB-0464-WJ-F(E)	Mouse Anti-CDH1 Recombinant Antibody (clone SC10.6); Fab Fragment	ELISA, FC	Mouse Fab

CAT	Product Name	Application	Type
HPAB-0465-WJ-F(E)	Mouse Anti-CDH1 Recombinant Antibody (clone SC10.15); Fab Fragment	ELISA, FC	Mouse Fab

CAT	Product Name	Application	Type
HPAB-0466-WJ-F(E)	Mouse Anti-CDH1 Recombinant Antibody (clone SC10.17); Fab Fragment	ELISA, FC	Mouse Fab

CAT	Product Name	Application	Type
HPAB-0467-WJ-F(E)	Human Anti-CDH1 Recombinant Antibody (clone hSC10.17); Fab Fragment	ELISA, FC	Humanized Fab

CAT	Product Name	Application	Type
HPAB-0464-WJ-S(P)	Mouse Anti-CDH1 Recombinant Antibody (clone SC10.6); scFv Fragment	ELISA, FC	Mouse scFv

CAT	Product Name	Application	Type
HPAB-0465-WJ-S(P)	Mouse Anti-CDH1 Recombinant Antibody (clone SC10.15); scFv Fragment	ELISA, FC	Mouse scFv

CAT	Product Name	Application	Type
HPAB-0466-WJ-S(P)	Mouse Anti-CDH1 Recombinant Antibody (clone SC10.17); scFv Fragment	ELISA, FC	Mouse scFv

CAT	Product Name	Application	Type
HPAB-0467-WJ-S(P)	Human Anti-CDH1 Recombinant Antibody (clone hSC10.17); scFv Fragment	ELISA, FC	Humanized scFv

CAT	Product Name	Application	Type
PABJ-0035-S(P)	Mouse Anti-CDH1 Recombinant Antibody scFv Fragment (PABJ-0035-S(P))	ELISA	Mouse scFv

CAT	Product Name	Application	Type
PABJ-0035-F(E)	Mouse Anti-CDH1 Recombinant Antibody Fab Fragment (PABJ-0035-F(E))	ELISA	Mouse Fab

CAT	Product Name	Application	Type
VS-0724-YC1204	AbPlus™ Anti-CDH1 Magnetic Beads (VS-0724-YC1204)	IP, Protein Purification

CAT	Product Name	Application	Type
VS-0724-YC1271	AbPlus™ Anti-Cdh1 Magnetic Beads (VS-0724-YC1271)	IP, Protein Purification

CAT	Product Name	Application	Type
VS13-YC191	CytoStream™ Rabbit Anti-CDH1 Recombinant Antibody (VS13-YC191)	WB, ICC, IF, IHC-P, FC, IP	Rabbit IgG

CAT	Product Name	Application	Type
VS-0425-YC562	Recombinant Anti-CDH1 Vesicular Antibody, EV Displayed (VS-0425-YC562)	ELISA, FC, Cell-uptake

CAT	Product Name	Application	Type
VS-0525-XY1278	Anti-CDH1 Immunohistochemistry Kit	IHC

CAT	Product Name	Application	Type
VS-0525-XY1279	Anti-Mouse CDH1 Immunohistochemistry Kit	IHC

CAT	Product Name	Application	Type
VS-0525-XY1281	Anti-Bovine CDH1 Immunohistochemistry Kit	IHC

CAT	Product Name	Application	Type
VS-1025-YC15	Anti-CDH1 Antibody Prodrug, Protease Activated (SC10.6)	ISZ, Cyt, FuncS

Specific Inquiry

Size:

Conjugation:

Endotoxin:

Purity:

Formats:

Isotype Switching:

Fc Engineering:

Modalities:

Add to compare

Request
Custom Production

See Our Custom Production in Action

Figure 1 Anti-HBV Recombinant Antibody (TAB-147) in SDS-PAGE

Download the full case study

Popular Products

Figure 2 Anti-Human EGFR Recombinant Antibody (TAB-020) in ELISA

Anti-Human EGFR Recombinant Antibody (Panitumumab) (CAT# TAB-020)
Application: ELISA, IP, FC, FuncS, Neut, IF, ICC

Figure 2 Anti-Human CD200 Recombinant Antibody (TAB-210) in HPLC

Anti-Human CD200 Recombinant Antibody (TAB-210) (CAT# TAB-210)
Application: IF, IP, Neut, FuncS, ELISA, FC, WB

Figure 2 Anti-TGF beta 2 Antibody (TAB-100) in HPLC

Anti-Human TGF beta 2 Recombinant Antibody (TAB-100) (CAT# TAB-100)
Application: ELISA, IP, FC, FuncS, Neut, IF, ICC

Figure 2 Anti-EGFR Recombinant Antibody (TAB-710) in ELISA

Anti-EGFR Recombinant Antibody (Nimotuzumab) (CAT# TAB-710)
Application: ELISA, IP, FC, FuncS, Neut, IF, IHC

Figure 2 Anti-Human CXCR4 Recombinant Antibody (TAB-H72) in HPLC

Human Anti-Human CXCR4 Recombinant Antibody (TAB-H72) (CAT# TAB-H72)
Application: IP, IF, FuncS, FC, Neut, ELISA, ICC

Figure 2 Anti-Human IL6R Antibody (TAB-026) in WB

Anti-Human IL6R Recombinant Antibody (TAB-026) (CAT# TAB-026)
Application: ELISA, FC, IP, FuncS, IF, Neut, ICC

Figure 2 HRV-C Antibody (MHH-513) in HPLC

Recombinant Human Anti-HRV-C Antibody (CAT# MHH-513)
Application: WB, Neut, FuncS

Figure 1 Trastuzumab (TP-083CL) in SPR

Human Anti-ERBB2 Rcombinant Antibody (TP-083CL) (CAT# TP-083CL)
Application:

Figure 1 Anti-Human CD200R Protein Antibody in ELISA

Human Anti-CD200R1 Recombinant Antibody (clone OX108) (CAT# PABC-020)
Application: WB, FuncS

Figure 2 Anti-Mouse TIGIT Recombinant Antibody (TAB-0279CL) in HPLC

Hamster Anti-TIGIT Recombinant Antibody (TAB-0279CL) (CAT# TAB-0279CL)
Application: FuncS

Figure 2 Anti-Human CD24 Recombinant Antibody (TAB-006LC) in WB

Mouse Anti-CD24 Recombinant Antibody (TAB-006LC) (CAT# TAB-006LC)
Application: FC, FuncS

Figure 2 CCP Recombinant Antibody (TAB-390CQ) in HPLC

Anti-Human CCP Recombinant Antibody (F12) (CAT# TAB-390CQ)
Application: ELISA, IHC

Figure 4 Anti-MUC1 Recombinant Antibody (TAB-439MZ) in WB

Anti-Human MUC1 Recombinant Antibody (1B2) (CAT# TAB-439MZ)
Application: FC

Figure 2 Human Anti-RSV F Recombinant Antibody (MRO-878LC) in ELISA

Human Anti-RSV F Recombinant Antibody (MRO-878LC) (CAT# MRO-878LC)
Application: ELISA, Neut

Figure 2 Anti-VEEV E2 Antibody (MRO-1164LC) in HPLC

Anti-VEEV E2 Recombinant Antibody (Hy4-26A) (CAT# MRO-1164LC)
Application: WB, ELISA

Figure 4 Anti-SIRPA Recombinant Antibody (NS-080CN) in ELISA

Human Anti-SIRPA Recombinant Antibody (clone 29-AM3-35) (CAT# NS-080CN)
Application: ELISA, Inhib, FuncS

Figure 3 Recombinant Mouse Anti-Pfs25 Antibody (4B7) in ELISA.

Recombinant Mouse Anti-Pfs25 Antibody (4B7) (CAT# FAMAB-0226-CN)
Application: ELISA, IB, WB, IF

Figure 2 Recombinant Mouse Anti-CD22 Antibody (HPAB-N0324-YC) in HPLC

Mouse Anti-CD22 Recombinant Antibody (HPAB-N0324-YC) (CAT# HPAB-N0324-YC)
Application: FuncS

Figure 7 Anti-EBV gH/gL Antibody (PABC-419) in ELISA

Recombinant Mouse Anti-EBV gH/gL Antibody (CL40) (CAT# PABC-419)
Application: ELISA, Inhib, FC, Neut

Figure 3 Human Anti-SIRPA Antibody (PABJ-0078) in DB

Human Anti-SIRPA Recombinant Antibody (PABJ-0078) (CAT# PABJ-0078)
Application: ELISA

For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.

Send Inquiry

This site is protected by reCAPTCHA and the Google Privacy Policy and Terms of Service apply.